Construct: sgRNA BRDN0001148930
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCATGAAGATTAATAACACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK17B (9262)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76797
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
196156498 |
+ |
STK17B |
NNNTGAAGATTAATAACACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
31855961 |
+ |
NUBPL |
NNNTGAAGATAAATAACACT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000003.12 |
3 |
170154159 |
- |
PHC3 |
NNNTGAAGATGAATAACACA |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000002.12 |
2 |
196376035 |
- |
HECW2 |
NNNTGGAGATTAATAAAACG |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000003.12 |
3 |
60451333 |
- |
FHIT |
NNNTGAAGATTTATAACACA |
NGG |
2 |
0.3125 |
Tier II |
| 6 |
NC_000011.10 |
11 |
131950320 |
+ |
NTM |
NNNTGCAGACTAATAACACG |
NGG |
2 |
0.2424 |
Tier II |
| 7 |
NC_000001.11 |
1 |
174273990 |
- |
RABGAP1L |
NNNTGAAGATTAACAACACT |
NGG |
2 |
0.2 |
Tier II |
| 8 |
NC_000002.12 |
2 |
138676310 |
- |
NXPH2 |
NNNTGATGATTAATAACAAG |
NGG |
2 |
0.1875 |
Tier II |
| 9 |
NC_000022.11 |
22 |
17162398 |
- |
HDHD5 |
NNNTGAAGATTAATAACACT |
NAG |
2 |
0.1815 |
Tier II |
| 10 |
NC_000002.12 |
2 |
65383796 |
+ |
SPRED2 |
NNNTGAAGATTAATAACCCA |
NGG |
2 |
0.1786 |
Tier II |
| 11 |
NC_000008.11 |
8 |
38439462 |
+ |
FGFR1 |
NNNAGAAGATTAAGAACACG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000010.11 |
10 |
9837017 |
- |
LINC02663 |
NNNTGAAGCATAATAACACG |
NGG |
2 |
0.4898 |
Tier III |
| 13 |
NC_000012.12 |
12 |
85488811 |
- |
LINC02820 |
NNNTGAAGATTAATAATACA |
NGG |
2 |
0.4375 |
Tier III |
| 14 |
NC_000022.11 |
22 |
17162398 |
- |
HDHD5-AS1 |
NNNTGAAGATTAATAACACT |
NAG |
2 |
0.1815 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
3651586 |
+ |
Hnf4g |
NNNTGAAGATTAAGAACATG |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
76954345 |
- |
Agbl1 |
NNNTGAAAATTAAAAACACG |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000076.6 |
10 |
13842859 |
- |
Aig1 |
NNNTGTAGATAAATAACACG |
NGG |
2 |
0.5357 |
Tier II |
| 4 |
NC_000086.7 |
X |
69591725 |
- |
Aff2 |
NNNTGAACATTAATAACACT |
NGG |
2 |
0.4308 |
Tier II |
| 5 |
NC_000076.6 |
10 |
73784920 |
- |
Pcdh15 |
NNNTTATGATTAATAACACG |
NGG |
2 |
0.1313 |
Tier II |
| 6 |
NC_000072.6 |
6 |
63861926 |
- |
Grid2 |
NNNTGAAGATTAATAACACA |
NTG |
2 |
0.0365 |
Tier II |
| 7 |
NC_000073.6 |
7 |
142344793 |
+ |
Ifitm10 |
NNNTGAAGTTTAATAACACG |
NTG |
2 |
0.0234 |
Tier II |
| 8 |
NC_000085.6 |
19 |
26499313 |
+ |
Gm34808 |
NNNTGAAGAATAAAAACACG |
NGG |
2 |
0.5306 |
Tier III |
| 9 |
NC_000068.7 |
2 |
75254623 |
+ |
2600014E21Rik |
NNNTGAAGATTAATACCATG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
