Construct: sgRNA BRDN0001148931
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGACGACGTGCAGTACCTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRRK1 (79705)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75717
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
101027720 |
+ |
LRRK1 |
NNNCGACGTGCAGTACCTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
58661359 |
- |
IGBP1C |
NNNCGACCTGAAGTACCTGA |
NGG |
2 |
0.4615 |
Tier I |
| 3 |
NC_000001.11 |
1 |
203179576 |
- |
CHI3L1 |
NNNCCAGGTGCAGTACCTGA |
NGG |
2 |
0.3697 |
Tier I |
| 4 |
NC_000015.10 |
15 |
71864369 |
- |
MYO9A |
NNNCCACCTGCAGTACCTGA |
NGG |
2 |
0.4835 |
Tier II |
| 5 |
NC_000017.11 |
17 |
58661359 |
- |
TEX14 |
NNNCGACCTGAAGTACCTGA |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000008.11 |
8 |
88415665 |
+ |
LOC105375630 |
NNNAGACCTGCAGTACCTGA |
NGG |
2 |
0.5182 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
100494952 |
+ |
Igbp1 |
NNNCGACCTGAAGTACCTGA |
NGG |
2 |
0.4615 |
Tier I |
| 2 |
NC_000067.6 |
1 |
136085291 |
- |
Cacna1s |
NNNCGATGTCCAGTACCTGA |
NGG |
2 |
0.325 |
Tier I |
| 3 |
NC_000071.6 |
5 |
62749569 |
+ |
Arap2 |
NNNCGACGTGCAGTCCCTCA |
NGG |
2 |
0.1223 |
Tier I |
| 4 |
NC_000080.6 |
14 |
8125608 |
+ |
Pxk |
NNNCAACGTGCAGTGCCTGA |
NGG |
2 |
0.5633 |
Tier II |
| 5 |
NC_000086.7 |
X |
53314883 |
+ |
Fam122c |
NNNCAACGTGCAGCACCTGA |
NGG |
2 |
0.2476 |
Tier II |
| 6 |
NC_000080.6 |
14 |
8125608 |
+ |
Gm18144 |
NNNCAACGTGCAGTGCCTGA |
NGG |
2 |
0.5633 |
Tier III |
| 7 |
NC_000086.7 |
X |
53314883 |
+ |
Gm14585 |
NNNCAACGTGCAGCACCTGA |
NGG |
2 |
0.2476 |
Tier III |
| 8 |
NC_000085.6 |
19 |
11091972 |
+ |
Gm336 |
NNNCGACGTGCAGTCCCTCA |
NGG |
2 |
0.1223 |
Tier III |
| 9 |
NC_000067.6 |
1 |
110475847 |
+ |
Gm3332 |
NNNCGACCTGCAGTACCTGA |
NGT |
2 |
0.0099 |
Tier III |
Other clones with same target sequence:
(none)
