Construct: sgRNA BRDN0001148932
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGGTTTCAAACCTAGTAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR2A (92)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77929
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
147915221 |
- |
ACVR2A |
NNNGGTTTCAAACCTAGTAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
7332854 |
- |
CAMTA1 |
NNNGGTTGCAAGCCTAGTAA |
NGG |
2 |
0.5296 |
Tier II |
| 3 |
NC_000013.11 |
13 |
75639498 |
+ |
LMO7 |
NNNGGTTTAAAACCAAGTAA |
NGG |
2 |
0.4962 |
Tier II |
| 4 |
NC_000009.12 |
9 |
69438588 |
- |
APBA1 |
NNNGGTTTCAATCCTAGTAT |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000005.10 |
5 |
172046545 |
+ |
STK10 |
NNNGGTTTCACAACTAGTAA |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000005.10 |
5 |
146671484 |
+ |
PPP2R2B |
NNNGCTTTCAAACCTGGTAA |
NGG |
2 |
0.1511 |
Tier II |
| 7 |
NC_000013.11 |
13 |
91723566 |
- |
GPC5 |
NNNGGTTTTAAACCTAGTAA |
NCG |
2 |
0.0937 |
Tier II |
| 8 |
NC_000011.10 |
11 |
43586599 |
+ |
HSD17B12 |
NNNGGTTTCAAACCCAGTAA |
NGA |
2 |
0.0189 |
Tier II |
| 9 |
NC_000009.12 |
9 |
69417579 |
- |
LOC124902177 |
NNNGGTTTCAATCCTAGTAT |
NGG |
2 |
0.2 |
Tier III |
| 10 |
NC_000002.12 |
2 |
73973180 |
- |
DGUOK-AS1 |
NNNGGTTTCAAGCCTAGTAA |
NTG |
2 |
0.0281 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
48890328 |
- |
Acvr2a |
NNNGGCTTCAACCCTAGTAA |
NGG |
2 |
0.2392 |
Tier I |
| 2 |
NC_000071.6 |
5 |
89860998 |
+ |
Adamts3 |
NNNGGTTTCTAACCTAGAAA |
NGG |
2 |
0.5882 |
Tier II |
| 3 |
NC_000079.6 |
13 |
104817949 |
+ |
Srek1ip1 |
NNNGGTTTCTAACCTAGAAA |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000084.6 |
18 |
64361456 |
- |
Onecut2 |
NNNGGTTACAGACCTAGTAA |
NGG |
2 |
0.52 |
Tier II |
| 5 |
NC_000076.6 |
10 |
10940290 |
+ |
Grm1 |
NNNTGTTTTAAACCTAGTAA |
NGG |
2 |
0.3182 |
Tier II |
| 6 |
NC_000073.6 |
7 |
81065357 |
+ |
Alpk3 |
NNNGGTTTCAAGCCTAGTGA |
NGG |
2 |
0.2708 |
Tier II |
| 7 |
NC_000070.6 |
4 |
59419991 |
- |
Susd1 |
NNNGGTTTGAAACCTAGCAA |
NGG |
2 |
0.2653 |
Tier II |
| 8 |
NC_000084.6 |
18 |
51272841 |
- |
Prr16 |
NNNGGTTTCAAAACTAGTTA |
NGG |
2 |
0.2071 |
Tier II |
| 9 |
NC_000076.6 |
10 |
81303848 |
- |
Pip5k1c |
NNNGATTTCAAACCTAGTAC |
NGG |
2 |
0.197 |
Tier II |
| 10 |
NC_000074.6 |
8 |
108838313 |
- |
Zfhx3 |
NNNGATTTCAAACCTGGTAA |
NGG |
2 |
0.1667 |
Tier II |
| 11 |
NC_000083.6 |
17 |
43550294 |
+ |
Ankrd66 |
NNNGGTTTCAAACCTAGTAA |
NTG |
1 |
0.039 |
Tier II |
| 12 |
NC_000080.6 |
14 |
19755370 |
+ |
Nid2 |
NNNGGCTTCAAACCTTGTAA |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000071.6 |
5 |
127099350 |
- |
Gm33491 |
NNNGGTTTGAAACCTAGTGA |
NGG |
2 |
0.2321 |
Tier III |
| 14 |
NC_000076.6 |
10 |
81303848 |
- |
Gm16315 |
NNNGATTTCAAACCTAGTAC |
NGG |
2 |
0.197 |
Tier III |
| 15 |
NC_000080.6 |
14 |
84068016 |
+ |
Gm32455 |
NNNGGTTTCAAACCCAGTAA |
NAG |
2 |
0.0707 |
Tier III |
| 16 |
NC_000069.6 |
3 |
74115926 |
- |
Gm36069 |
NNNTGTTTCAAACCTAGTAA |
NGT |
2 |
0.0059 |
Tier III |
| 17 |
NC_000074.6 |
8 |
39912739 |
+ |
Gm36795 |
NNNGGTTTCAAAGCTAGTAA |
NTG |
2 |
0.0053 |
Tier III |
Other clones with same target sequence:
(none)
