Construct: sgRNA BRDN0001148940
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGCACGGCATACTCCCAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STRADB (55437)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75496
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
201473004 |
+ |
STRADB |
NNNGCACGGCATACTCCCAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
48436209 |
+ |
SPATA6 |
NNNGCATGGCATACTCCCAC |
NGG |
1 |
0.8125 |
Tier II |
| 3 |
NC_000007.14 |
7 |
14397292 |
- |
DGKB |
NNNGAACGGCAAACTCCCAC |
NGG |
2 |
0.4571 |
Tier II |
| 4 |
NC_000016.10 |
16 |
83990662 |
- |
NECAB2 |
NNNGCACGGCATACCCCCAT |
NGG |
2 |
0.0818 |
Tier II |
| 5 |
NC_000011.10 |
11 |
62880354 |
- |
SLC3A2 |
NNNGCACGGCTTACTCCCAC |
NCG |
2 |
0.033 |
Tier II |
| 6 |
NC_000006.12 |
6 |
110735995 |
+ |
CDK19 |
NNNGCACGGCATACTCCCTC |
NGC |
2 |
0.012 |
Tier II |
| 7 |
NC_000001.11 |
1 |
48436209 |
+ |
LOC644462 |
NNNGCATGGCATACTCCCAC |
NGG |
1 |
0.8125 |
Tier III |
| 8 |
NC_000007.14 |
7 |
150513575 |
- |
STRADBP1 |
NNNGCACGGCATACTCCTAC |
NGG |
1 |
0.6429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
58988555 |
+ |
Stradb |
NNNGCACGGCACACTCCTAC |
NGG |
2 |
0.609 |
Tier I |
| 2 |
NC_000075.6 |
9 |
108064147 |
+ |
Rnf123 |
NNNGCACAGCTTACTCCCAC |
NGG |
2 |
0.3077 |
Tier II |
| 3 |
NC_000068.7 |
2 |
179822910 |
+ |
Cdh4 |
NNNGCACTGGATACTCCCAC |
NGG |
2 |
0.2431 |
Tier II |
| 4 |
NC_000079.6 |
13 |
100009175 |
- |
Mccc2 |
NNNGCACAGCATACTGCCAC |
NGG |
2 |
0.1538 |
Tier II |
| 5 |
NC_000077.6 |
11 |
76543478 |
- |
Abr |
NNNGCACGGCATGCTCCCAG |
NGG |
2 |
0.0384 |
Tier II |
| 6 |
NC_000081.6 |
15 |
73688730 |
- |
Gpr20 |
NNNCCACGGCATACTCCCAC |
NTG |
2 |
0.0206 |
Tier II |
| 7 |
NC_000072.6 |
6 |
120789547 |
+ |
Slc25a18 |
NNNGCACTGCATAGTCCCAC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000068.7 |
2 |
117880573 |
+ |
4930412B13Rik |
NNNACACGGCATACTCTCAC |
NGG |
2 |
0.42 |
Tier III |
| 9 |
NC_000068.7 |
2 |
117880573 |
+ |
Gm13986 |
NNNACACGGCATACTCTCAC |
NGG |
2 |
0.42 |
Tier III |
Other clones with same target sequence:
(none)
