Construct: sgRNA BRDN0001148941
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGAGGCACCAGGAGACCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKACG (5568)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77365
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
69013896 |
- |
PRKACG |
NNNAGGCACCAGGAGACCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
31659566 |
- |
C6orf47 |
NNNTGGCACCAGGAGACCAG |
NGG |
2 |
0.4545 |
Tier I |
| 3 |
NC_000014.9 |
14 |
105143171 |
+ |
JAG2 |
NNNAGGCACCAGCAGACCTG |
NGG |
2 |
0.2807 |
Tier I |
| 4 |
NC_000012.12 |
12 |
130437147 |
+ |
RIMBP2 |
NNNAGGCACCAGGAGCTCGG |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000022.11 |
22 |
50024788 |
+ |
TTLL8 |
NNNAAGCACCAGGAGACCTG |
NGG |
2 |
0.5778 |
Tier II |
| 6 |
NC_000019.10 |
19 |
40261092 |
+ |
AKT2 |
NNNAGACACCAGGAGACAGG |
NGG |
2 |
0.5385 |
Tier II |
| 7 |
NC_000009.12 |
9 |
123643811 |
- |
DENND1A |
NNNAGGCACCAGGAAACAGG |
NGG |
2 |
0.5068 |
Tier II |
| 8 |
NC_000016.10 |
16 |
81606482 |
- |
CMIP |
NNNAGGCACCAGGTGACCGA |
NGG |
2 |
0.5 |
Tier II |
| 9 |
NC_000014.9 |
14 |
105156577 |
+ |
JAG2 |
NNNAGCCACCAGGAGACCAG |
NGG |
2 |
0.487 |
Tier II |
| 10 |
NC_000022.11 |
22 |
50160915 |
+ |
MOV10L1 |
NNNAGGCACCAGGAGACATG |
NGG |
2 |
0.359 |
Tier II |
| 11 |
NC_000010.11 |
10 |
20011265 |
- |
PLXDC2 |
NNNAGCCACCAGGAGAACGG |
NGG |
2 |
0.3182 |
Tier II |
| 12 |
NC_000009.12 |
9 |
16829061 |
+ |
BNC2 |
NNNAGGCACCAGGGGACCTG |
NGG |
2 |
0.3111 |
Tier II |
| 13 |
NC_000010.11 |
10 |
124666870 |
- |
FAM53B |
NNNAGGCCCCAGGAGACCTG |
NGG |
2 |
0.2857 |
Tier II |
| 14 |
NC_000017.11 |
17 |
44928656 |
- |
KIF18B |
NNNAGGCCCCAGGAGACCTG |
NGG |
2 |
0.2857 |
Tier II |
| 15 |
NC_000018.10 |
18 |
36447948 |
+ |
FHOD3 |
NNNAGGGACCAGGAGACAGG |
NGG |
2 |
0.2534 |
Tier II |
| 16 |
NC_000019.10 |
19 |
40720670 |
- |
ITPKC |
NNNAGGCGCCTGGAGACCGG |
NGG |
2 |
0.2256 |
Tier II |
| 17 |
NC_000019.10 |
19 |
43736429 |
- |
SMG9 |
NNNAGGCCCCAGGAGACCGC |
NGG |
2 |
0.1837 |
Tier II |
| 18 |
NC_000008.11 |
8 |
123269330 |
+ |
ZHX1 |
NNNAGGCACCAGGATACCAG |
NGG |
2 |
0.102 |
Tier II |
| 19 |
NC_000008.11 |
8 |
123269330 |
+ |
ZHX1-C8orf76 |
NNNAGGCACCAGGATACCAG |
NGG |
2 |
0.102 |
Tier II |
| 20 |
NC_000017.11 |
17 |
81574605 |
+ |
NPLOC4 |
NNNAGGCACCAGGAGGTCGG |
NGG |
2 |
0.0897 |
Tier II |
| 21 |
NC_000001.11 |
1 |
236008448 |
+ |
NID1 |
NNNAGGCACCAGGAGACCAG |
NTG |
2 |
0.0278 |
Tier II |
| 22 |
NC_000008.11 |
8 |
69624981 |
- |
SULF1 |
NNNAGGCACCAGGAGACCAG |
NGC |
2 |
0.0159 |
Tier II |
| 23 |
NC_000017.11 |
17 |
45919253 |
- |
MAPT |
NNNAGGCACCAGGGGCCCGG |
NGG |
2 |
0.0 |
Tier II |
| 24 |
NC_000020.11 |
20 |
58762827 |
- |
PIEZO1P2 |
NNNAGGCACCAGGAGACCTA |
NGG |
2 |
0.625 |
Tier III |
| 25 |
NC_000017.11 |
17 |
63913440 |
- |
LOC112268204 |
NNNGGGCACCAGGAGACAGG |
NGG |
2 |
0.3365 |
Tier III |
| 26 |
NC_000006.12 |
6 |
170030881 |
- |
LOC105378150 |
NNNAGGCACCAGGAAGCCGG |
NGG |
2 |
0.181 |
Tier III |
| 27 |
NC_000005.10 |
5 |
2117375 |
+ |
LOC105374618 |
NNNAGGCACCAGGAGACCTG |
NTG |
2 |
0.026 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
64194784 |
- |
Cdc14b |
NNNAGGCACCAGGAGACAGA |
NGG |
2 |
0.5048 |
Tier II |
| 2 |
NC_000083.6 |
17 |
8932840 |
- |
Pde10a |
NNNAGGCACCAGGAAATCGG |
NGG |
2 |
0.4392 |
Tier II |
| 3 |
NC_000085.6 |
19 |
55769354 |
- |
Tcf7l2 |
NNNAGGCACCAGGAAATCGG |
NGG |
2 |
0.4392 |
Tier II |
| 4 |
NC_000071.6 |
5 |
135178405 |
+ |
Bcl7b |
NNNAGGCTCCAGGAAACCGG |
NGG |
2 |
0.4034 |
Tier II |
| 5 |
NC_000082.6 |
16 |
10432592 |
+ |
Tvp23a |
NNNAGTCACCAGGAGACAGG |
NGG |
2 |
0.359 |
Tier II |
| 6 |
NC_000070.6 |
4 |
108714278 |
- |
Zfyve9 |
NNNAGGCAGCAGGAGACAGG |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000078.6 |
12 |
84806640 |
- |
Ltbp2 |
NNNAGGCACCAGGAGATTGG |
NGG |
2 |
0.3 |
Tier II |
| 8 |
NC_000070.6 |
4 |
134888118 |
- |
Rhd |
NNNAGGCACCAGGACACCGA |
NGG |
2 |
0.2557 |
Tier II |
| 9 |
NC_000083.6 |
17 |
8739983 |
- |
Pde10a |
NNNAGGCACCAGGACACCTG |
NGG |
2 |
0.1818 |
Tier II |
| 10 |
NC_000082.6 |
16 |
22150005 |
+ |
Igf2bp2 |
NNNAGGCACAAGGAGGCCGG |
NGG |
2 |
0.1667 |
Tier II |
| 11 |
NC_000067.6 |
1 |
22314116 |
- |
Rims1 |
NNNAGGAACCAGGAGGCCGG |
NGG |
2 |
0.1442 |
Tier II |
| 12 |
NC_000082.6 |
16 |
33947696 |
- |
Itgb5 |
NNNAGGGACCAGGAGACGGG |
NGG |
2 |
0.0627 |
Tier II |
| 13 |
NC_000074.6 |
8 |
14851434 |
+ |
Dlgap2 |
NNNAGGCACCAGGAGCCCCG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000070.6 |
4 |
56658780 |
+ |
Gm34687 |
NNNAGGTACCAGGAGACAGG |
NGG |
2 |
0.4375 |
Tier III |
| 15 |
NC_000076.6 |
10 |
118024999 |
+ |
Gm32663 |
NNNAGGCTCCAGGAGACCAG |
NGG |
2 |
0.3061 |
Tier III |
Other clones with same target sequence:
(none)
