Construct: sgRNA BRDN0001148944
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCCAACCTGTATTTCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TSSK4 (283629)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77046
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
24206140 |
- |
TSSK4 |
NNNCCAACCTGTATTTCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
113501098 |
- |
TMCO3 |
NNNCCAAACTGTAATTCACG |
NGG |
2 |
0.4024 |
Tier II |
| 3 |
NC_000010.11 |
10 |
27980752 |
- |
ODAD2 |
NNNCCAACCTGTTTTCCACG |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000016.10 |
16 |
56231805 |
+ |
GNAO1 |
NNNCCAACCTGTATTTCCCA |
NGG |
2 |
0.1786 |
Tier II |
| 5 |
NC_000008.11 |
8 |
144525036 |
- |
LRRC14 |
NNNCCAACCTGTGTTTCAGG |
NGG |
2 |
0.0815 |
Tier II |
| 6 |
NC_000008.11 |
8 |
144525036 |
- |
LRRC24 |
NNNCCAACCTGTGTTTCAGG |
NGG |
2 |
0.0815 |
Tier II |
| 7 |
NC_000004.12 |
4 |
177806929 |
+ |
LINC01098 |
NNNCCAACCTGTATTTCATG |
NGT |
2 |
0.0074 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
34746909 |
+ |
Fam50b |
NNNCCAACCTGTCTTTCACG |
NTG |
2 |
0.0082 |
Tier I |
| 2 |
NC_000069.6 |
3 |
139358917 |
- |
Stpg2 |
NNNCCAACCTGAATTACACG |
NGG |
2 |
0.7273 |
Tier II |
| 3 |
NC_000079.6 |
13 |
52593860 |
- |
Syk |
NNNACAAGCTGTATTTCACG |
NGG |
2 |
0.5414 |
Tier II |
| 4 |
NC_000084.6 |
18 |
22890820 |
- |
Nol4 |
NNNCCATTCTGTATTTCACG |
NGG |
2 |
0.3828 |
Tier II |
| 5 |
NC_000084.6 |
18 |
84234875 |
- |
Zfp407 |
NNNCCAAGCTGTATTTCATG |
NGG |
2 |
0.2967 |
Tier II |
| 6 |
NC_000076.6 |
10 |
64336132 |
- |
Ctnna3 |
NNNCCAACCTGTATTTCACG |
NTT |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
