Construct: sgRNA BRDN0001148949
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACCCCATGGACTACAAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AKT2 (208)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77506
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
40242607 |
- |
AKT2 |
NNNCCCATGGACTACAAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
15455983 |
- |
PIR |
NNNCCCATGGCCTACAACTG |
NGG |
2 |
0.1905 |
Tier I |
| 3 |
NC_000008.11 |
8 |
20201528 |
- |
ATP6V1B2 |
NNNCACTTGGACTACAAGTG |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000023.11 |
X |
12094510 |
- |
FRMPD4 |
NNNCCCATGGACTGCAAGTC |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000007.14 |
7 |
100061562 |
- |
ZSCAN21 |
NNNGCCATGGACTACATGTG |
NGG |
2 |
0.0667 |
Tier II |
| 6 |
NC_000023.11 |
X |
15455983 |
- |
PIR-FIGF |
NNNCCCATGGCCTACAACTG |
NGG |
2 |
0.1905 |
Tier III |
| 7 |
NC_000019.10 |
19 |
31100506 |
- |
LOC124900621 |
NNNCCCAGGGACTACAAGGG |
NGG |
2 |
0.1833 |
Tier III |
| 8 |
NC_000011.10 |
11 |
30032341 |
- |
LOC107984321 |
NNNCCCATGGCCTACAAGTG |
NCG |
2 |
0.0429 |
Tier III |
| 9 |
NC_000001.11 |
1 |
200693436 |
- |
DDX59-AS1 |
NNNCCCATGGACTAAGAGTG |
NGG |
2 |
0.0427 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
27628918 |
+ |
Akt2 |
NNNGCCATGGATTACAAGTG |
NGG |
2 |
0.2692 |
Tier I |
| 2 |
NC_000072.6 |
6 |
113971788 |
- |
Atp2b2 |
NNNCCTATGGACTACAAGTG |
NGG |
1 |
0.9286 |
Tier II |
| 3 |
NC_000072.6 |
6 |
63543596 |
- |
Grid2 |
NNNCCCAAGGACTACAAGTG |
NGG |
1 |
0.8 |
Tier II |
| 4 |
NC_000078.6 |
12 |
30176560 |
- |
Sntg2 |
NNNCCCAAGGACTACAACTG |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000067.6 |
1 |
181578545 |
- |
Dnah14 |
NNNCCCAAGGAGTACAAGTG |
NGG |
2 |
0.3556 |
Tier II |
| 6 |
NC_000069.6 |
3 |
90226172 |
+ |
Rab13 |
NNNCCCAAGGACTACAAGAG |
NGG |
2 |
0.2286 |
Tier II |
| 7 |
NC_000069.6 |
3 |
81971229 |
- |
Tdo2 |
NNNCCCAGGGACTACAAGTG |
NAG |
2 |
0.1901 |
Tier II |
| 8 |
NC_000080.6 |
14 |
119043453 |
+ |
Uggt2 |
NNNCCCATGGCGTACAAGTG |
NGG |
2 |
0.1778 |
Tier II |
| 9 |
NC_000076.6 |
10 |
93005676 |
- |
Cfap54 |
NNNCCCATGTACTCCAAGTG |
NGG |
2 |
0.1741 |
Tier II |
| 10 |
NC_000073.6 |
7 |
132664383 |
+ |
Lhpp |
NNNCCCATTGTCTACAAGTG |
NGG |
2 |
0.1641 |
Tier II |
| 11 |
NC_000067.6 |
1 |
73999675 |
- |
Tns1 |
NNNCCCAGGGACTCCAAGTG |
NGG |
2 |
0.1571 |
Tier II |
| 12 |
NC_000069.6 |
3 |
117649109 |
+ |
Plppr5 |
NNNGCCATGGACTACAAGTG |
NAG |
2 |
0.1296 |
Tier II |
| 13 |
NC_000067.6 |
1 |
34230281 |
+ |
Dst |
NNNCCCATGGACTGAAAGTG |
NGG |
2 |
0.1037 |
Tier II |
| 14 |
NC_000072.6 |
6 |
125774710 |
- |
Ano2 |
NNNCCCATGTACTAGAAGTG |
NGG |
2 |
0.0406 |
Tier II |
| 15 |
NC_000067.6 |
1 |
178750495 |
- |
Kif26b |
NNNTCCATGGACTACAAGTG |
NTG |
2 |
0.0312 |
Tier II |
| 16 |
NC_000078.6 |
12 |
113021184 |
- |
Pacs2 |
NNNTCCATGGACTACAAGTG |
NTG |
2 |
0.0312 |
Tier II |
| 17 |
NC_000073.6 |
7 |
35003813 |
+ |
Pepd |
NNNCCCATGGAGTAGAAGTG |
NGG |
2 |
0.0222 |
Tier II |
| 18 |
NC_000080.6 |
14 |
63094553 |
- |
Gm36025 |
NNNGCCATGGACCACAAGTG |
NGG |
2 |
0.3947 |
Tier III |
| 19 |
NC_000077.6 |
11 |
16514904 |
+ |
Akt2-ps |
NNNGCCATGGATTACAAGTG |
NGG |
2 |
0.2692 |
Tier III |
| 20 |
NC_000067.6 |
1 |
178750495 |
- |
Gm34176 |
NNNTCCATGGACTACAAGTG |
NTG |
2 |
0.0312 |
Tier III |
Other clones with same target sequence:
(none)
