Construct: sgRNA BRDN0001148951
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCAGGACAGCCAATACAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDK1 (5163)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77010
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
172568765 |
+ |
PDK1 |
NNNAGGACAGCCAATACAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
183736800 |
+ |
YEATS2 |
NNNAGGACAGCCAGAACAAG |
NGG |
2 |
0.2702 |
Tier I |
| 3 |
NC_000011.10 |
11 |
2441365 |
- |
TRPM5 |
NNNAGGACAGACAATACAAG |
NGG |
1 |
0.75 |
Tier II |
| 4 |
NC_000011.10 |
11 |
14888892 |
- |
CYP2R1 |
NNNAGGAAAGCCAATACAAG |
NGG |
1 |
0.65 |
Tier II |
| 5 |
NC_000023.11 |
X |
97243286 |
- |
DIAPH2 |
NNNAAGACAGCAAATACAAG |
NGG |
2 |
0.619 |
Tier II |
| 6 |
NC_000012.12 |
12 |
21464334 |
- |
PYROXD1 |
NNNAGAACAGCTAATACAAG |
NGG |
2 |
0.5385 |
Tier II |
| 7 |
NC_000006.12 |
6 |
143195089 |
+ |
AIG1 |
NNNAGAACAGCCAATAAAAG |
NGG |
2 |
0.4667 |
Tier II |
| 8 |
NC_000009.12 |
9 |
20943469 |
- |
FOCAD |
NNNAGCACGGCCAATACAAG |
NGG |
2 |
0.4545 |
Tier II |
| 9 |
NC_000002.12 |
2 |
215413594 |
- |
FN1 |
NNNAGGACAGCCAATACAGA |
NGG |
2 |
0.3516 |
Tier II |
| 10 |
NC_000023.11 |
X |
10689802 |
- |
MID1 |
NNNAGCACAGCCAATAAAAG |
NGG |
2 |
0.3182 |
Tier II |
| 11 |
NC_000015.10 |
15 |
56639843 |
+ |
ZNF280D |
NNNAGGACATCCAATACAAG |
NGA |
2 |
0.0564 |
Tier II |
| 12 |
NC_000016.10 |
16 |
73634674 |
+ |
ZFHX3 |
NNNAGGACAGCCAACAGAAG |
NGG |
2 |
0.016 |
Tier II |
| 13 |
NC_000019.10 |
19 |
44915182 |
- |
APOC1 |
NNNAGGACAGCCAATCCCAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000011.10 |
11 |
14888892 |
- |
LOC124902638 |
NNNAGGAAAGCCAATACAAG |
NGG |
1 |
0.65 |
Tier III |
| 15 |
NC_000009.12 |
9 |
22112234 |
+ |
CDKN2B-AS1 |
NNNAGAACAGCCAAAACAAG |
NGG |
2 |
0.5789 |
Tier III |
| 16 |
NC_000023.11 |
X |
81600803 |
+ |
PDK1P2 |
NNNAGGACAACCAATATAAG |
NGG |
2 |
0.4356 |
Tier III |
| 17 |
NC_000020.11 |
20 |
23309786 |
+ |
NXT1-AS1 |
NNNAGGACAACCAATCCAAG |
NGG |
2 |
0.0 |
Tier III |
| 18 |
NC_000019.10 |
19 |
44927266 |
- |
APOC1P1 |
NNNAGGACAGCCAATCCCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
71887406 |
+ |
Pdk1 |
NNNAGGACAGACAATACAAG |
NGG |
1 |
0.75 |
Tier I |
| 2 |
NC_000082.6 |
16 |
20171273 |
+ |
Yeats2 |
NNNAGGACAGCCAGAACAAG |
NGG |
2 |
0.2702 |
Tier I |
| 3 |
NC_000082.6 |
16 |
50278560 |
+ |
Bbx |
NNNAGAACAGACAATACAAG |
NGG |
2 |
0.75 |
Tier II |
| 4 |
NC_000077.6 |
11 |
74952976 |
+ |
Smg6 |
NNNAGGACGGCCAATACAAG |
NGG |
1 |
0.6667 |
Tier II |
| 5 |
NC_000072.6 |
6 |
50197790 |
+ |
Mpp6 |
NNNTGAACAGCCAATACAAG |
NGG |
2 |
0.6364 |
Tier II |
| 6 |
NC_000067.6 |
1 |
179766329 |
- |
Ahctf1 |
NNNAGAACAGCCAGTACAAG |
NGG |
2 |
0.4667 |
Tier II |
| 7 |
NC_000068.7 |
2 |
70136004 |
- |
Myo3b |
NNNAGGACAGCCAATACAGG |
NGG |
1 |
0.375 |
Tier II |
| 8 |
NC_000072.6 |
6 |
117909192 |
+ |
Hnrnpf |
NNNAGCACAGCTAATACAAG |
NGG |
2 |
0.3671 |
Tier II |
| 9 |
NC_000079.6 |
13 |
19772179 |
- |
Gpr141 |
NNNAGGACAGACAATAAAAG |
NGG |
2 |
0.35 |
Tier II |
| 10 |
NC_000075.6 |
9 |
107667918 |
+ |
Slc38a3 |
NNNAGGACAGCGAAAACAAG |
NGG |
2 |
0.2573 |
Tier II |
| 11 |
NC_000072.6 |
6 |
125664973 |
- |
Vwf |
NNNGTGACAGCCAATACAAG |
NGG |
2 |
0.1875 |
Tier II |
| 12 |
NC_000076.6 |
10 |
24825561 |
+ |
Enpp3 |
NNNAGGACAGCCAATAAAGG |
NGG |
2 |
0.175 |
Tier II |
| 13 |
NC_000073.6 |
7 |
120768059 |
+ |
Vwa3a |
NNNAGGACAGCTTATACAAG |
NGG |
2 |
0.1615 |
Tier II |
| 14 |
NC_000078.6 |
12 |
17286992 |
- |
Atp6v1c2 |
NNNAGGACAGTCAGTACAAG |
NGG |
2 |
0.1436 |
Tier II |
| 15 |
NC_000068.7 |
2 |
65644072 |
- |
Scn2a |
NNNAGGACAGTGAATACAAG |
NGG |
2 |
0.1368 |
Tier II |
| 16 |
NC_000074.6 |
8 |
23857300 |
- |
Zmat4 |
NNNAGGTCAGCCAATGCAAG |
NGG |
2 |
0.0841 |
Tier II |
| 17 |
NC_000073.6 |
7 |
112831498 |
- |
Tead1 |
NNNAGGACAGGCAACACAAG |
NGG |
2 |
0.0682 |
Tier II |
| 18 |
NC_000075.6 |
9 |
106332051 |
+ |
Poc1a |
NNNAGGACAGCCAATAGAAG |
NGG |
1 |
0.0588 |
Tier II |
| 19 |
NC_000079.6 |
13 |
96507569 |
+ |
Polk |
NNNAGGACAGCCAAAAGAAG |
NGG |
2 |
0.0341 |
Tier II |
| 20 |
NC_000067.6 |
1 |
152969579 |
+ |
Nmnat2 |
NNNAAGACAGCCAATACAAG |
NTG |
2 |
0.0338 |
Tier II |
| 21 |
NC_000071.6 |
5 |
96559521 |
- |
Fras1 |
NNNAGTACAGCCAAGACAAG |
NGG |
2 |
0.0333 |
Tier II |
| 22 |
NC_000071.6 |
5 |
52839398 |
+ |
Anapc4 |
NNNAGGACAGCCTATAGAAG |
NGG |
2 |
0.0176 |
Tier II |
| 23 |
NC_000086.7 |
X |
35961001 |
- |
Dock11 |
NNNAGGACAGGCAATACAAG |
NGA |
2 |
0.0174 |
Tier II |
| 24 |
NC_000075.6 |
9 |
45249412 |
- |
BC049352 |
NNNATGACAGCCAATACAAG |
NTG |
2 |
0.0117 |
Tier II |
| 25 |
NC_000080.6 |
14 |
118753565 |
+ |
Gm46484 |
NNNAGGACAGCCAATACTAT |
NGG |
2 |
0.35 |
Tier III |
| 26 |
NC_000084.6 |
18 |
44325502 |
- |
Gm41717 |
NNNAGGTCAGCCAGTACAAG |
NGG |
2 |
0.2042 |
Tier III |
Other clones with same target sequence:
(none)
