Construct: sgRNA BRDN0001148953
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCGGGAGCTTGGCCTACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RAB11FIP5 (26056)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76187
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
73088947 |
- |
RAB11FIP5 |
NNNGGGAGCTTGGCCTACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
15478989 |
- |
COLQ |
NNNGGGAGCTTGGCCGACCA |
NGA |
2 |
0.024 |
Tier I |
| 3 |
NC_000003.12 |
3 |
58118075 |
+ |
FLNB |
NNNGGGAACTTGGCCAACCA |
NGG |
2 |
0.9091 |
Tier II |
| 4 |
NC_000012.12 |
12 |
103112798 |
- |
C12orf42 |
NNNGAGAGCTTGGCATACCA |
NGG |
2 |
0.1926 |
Tier II |
| 5 |
NC_000007.14 |
7 |
569540 |
+ |
PRKAR1B |
NNNGGGAGCTTTGCCTACAA |
NGG |
2 |
0.1648 |
Tier II |
| 6 |
NC_000016.10 |
16 |
49623696 |
- |
ZNF423 |
NNNGGGAGCTTGGCCTGACA |
NGG |
2 |
0.095 |
Tier II |
| 7 |
NC_000017.11 |
17 |
50610185 |
- |
CACNA1G |
NNNGGGAGCTTGGCCGGCCA |
NGG |
2 |
0.0611 |
Tier II |
| 8 |
NC_000008.11 |
8 |
23318788 |
- |
LOXL2 |
NNNGGGAGCTTGGCCTCCCC |
NGG |
2 |
0.0401 |
Tier II |
| 9 |
NC_000011.10 |
11 |
62789764 |
+ |
TMEM223 |
NNNGTGAGCTTGGCCTACCA |
NTG |
2 |
0.0117 |
Tier II |
| 10 |
NC_000011.10 |
11 |
62789764 |
+ |
TMEM179B |
NNNGTGAGCTTGGCCTACCA |
NTG |
2 |
0.0117 |
Tier II |
| 11 |
NC_000016.10 |
16 |
27170419 |
- |
LINC02129 |
NNNTGGGGCTTGGCCTACCA |
NGG |
2 |
0.2567 |
Tier III |
| 12 |
NC_000004.12 |
4 |
24984748 |
- |
LOC102723675 |
NNNGGGAGCTTAGCCTGCCA |
NGG |
2 |
0.1647 |
Tier III |
| 13 |
NC_000011.10 |
11 |
57163932 |
+ |
LOC105369309 |
NNNTGGAGCTTGGCCTTCCA |
NGG |
2 |
0.0485 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
20380104 |
- |
Rp2 |
NNNGGGAGGTTGGCCAACCA |
NGG |
2 |
0.5628 |
Tier II |
| 2 |
NC_000086.7 |
X |
71422919 |
+ |
Cd99l2 |
NNNGGTACCTTGGCCTACCA |
NGG |
2 |
0.4103 |
Tier II |
| 3 |
NC_000078.6 |
12 |
108627744 |
- |
Evl |
NNNGAGAGCTGGGCCTACCA |
NGG |
2 |
0.3467 |
Tier II |
| 4 |
NC_000071.6 |
5 |
22100010 |
- |
Reln |
NNNGGGAGCTTGGTCTACTA |
NGG |
2 |
0.3385 |
Tier II |
| 5 |
NC_000080.6 |
14 |
69458820 |
+ |
Gm21451 |
NNNGGGAGCTTAGCCTCCCA |
NGG |
2 |
0.1647 |
Tier II |
| 6 |
NC_000080.6 |
14 |
69458820 |
+ |
Gm16867 |
NNNGGGAGCTTAGCCTCCCA |
NGG |
2 |
0.1647 |
Tier II |
| 7 |
NC_000080.6 |
14 |
69677095 |
+ |
Loxl2 |
NNNGGGAGCTTAGCCTCCCA |
NGG |
2 |
0.1647 |
Tier II |
| 8 |
NC_000070.6 |
4 |
137964521 |
- |
Ece1 |
NNNGGGAGCATGGCCTGCCA |
NGG |
2 |
0.1513 |
Tier II |
| 9 |
NC_000072.6 |
6 |
82757049 |
+ |
Hk2 |
NNNGGGAGCTTGGCACACCA |
NGG |
2 |
0.1481 |
Tier II |
| 10 |
NC_000072.6 |
6 |
91842250 |
- |
Grip2 |
NNNGGGAGCTTTGCCTGCCA |
NGG |
2 |
0.0679 |
Tier II |
| 11 |
NC_000075.6 |
9 |
60988420 |
- |
Gm51680 |
NNNGGGAGCTTTTCCTACCA |
NGG |
2 |
0.1154 |
Tier III |
Other clones with same target sequence:
(none)
