Construct: sgRNA BRDN0001148953

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCGGGAGCTTGGCCTACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB11FIP5 (26056)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76187

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 73088947 - RAB11FIP5 NNNGGGAGCTTGGCCTACCA NGG 0 1.0 Tier I
2 NC_000003.12 3 15478989 - COLQ NNNGGGAGCTTGGCCGACCA NGA 2 0.024 Tier I
3 NC_000003.12 3 58118075 + FLNB NNNGGGAACTTGGCCAACCA NGG 2 0.9091 Tier II
4 NC_000012.12 12 103112798 - C12orf42 NNNGAGAGCTTGGCATACCA NGG 2 0.1926 Tier II
5 NC_000007.14 7 569540 + PRKAR1B NNNGGGAGCTTTGCCTACAA NGG 2 0.1648 Tier II
6 NC_000016.10 16 49623696 - ZNF423 NNNGGGAGCTTGGCCTGACA NGG 2 0.095 Tier II
7 NC_000017.11 17 50610185 - CACNA1G NNNGGGAGCTTGGCCGGCCA NGG 2 0.0611 Tier II
8 NC_000008.11 8 23318788 - LOXL2 NNNGGGAGCTTGGCCTCCCC NGG 2 0.0401 Tier II
9 NC_000011.10 11 62789764 + TMEM223 NNNGTGAGCTTGGCCTACCA NTG 2 0.0117 Tier II
10 NC_000011.10 11 62789764 + TMEM179B NNNGTGAGCTTGGCCTACCA NTG 2 0.0117 Tier II
11 NC_000016.10 16 27170419 - LINC02129 NNNTGGGGCTTGGCCTACCA NGG 2 0.2567 Tier III
12 NC_000004.12 4 24984748 - LOC102723675 NNNGGGAGCTTAGCCTGCCA NGG 2 0.1647 Tier III
13 NC_000011.10 11 57163932 + LOC105369309 NNNTGGAGCTTGGCCTTCCA NGG 2 0.0485 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 20380104 - Rp2 NNNGGGAGGTTGGCCAACCA NGG 2 0.5628 Tier II
2 NC_000086.7 X 71422919 + Cd99l2 NNNGGTACCTTGGCCTACCA NGG 2 0.4103 Tier II
3 NC_000078.6 12 108627744 - Evl NNNGAGAGCTGGGCCTACCA NGG 2 0.3467 Tier II
4 NC_000071.6 5 22100010 - Reln NNNGGGAGCTTGGTCTACTA NGG 2 0.3385 Tier II
5 NC_000080.6 14 69458820 + Gm21451 NNNGGGAGCTTAGCCTCCCA NGG 2 0.1647 Tier II
6 NC_000080.6 14 69458820 + Gm16867 NNNGGGAGCTTAGCCTCCCA NGG 2 0.1647 Tier II
7 NC_000080.6 14 69677095 + Loxl2 NNNGGGAGCTTAGCCTCCCA NGG 2 0.1647 Tier II
8 NC_000070.6 4 137964521 - Ece1 NNNGGGAGCATGGCCTGCCA NGG 2 0.1513 Tier II
9 NC_000072.6 6 82757049 + Hk2 NNNGGGAGCTTGGCACACCA NGG 2 0.1481 Tier II
10 NC_000072.6 6 91842250 - Grip2 NNNGGGAGCTTTGCCTGCCA NGG 2 0.0679 Tier II
11 NC_000075.6 9 60988420 - Gm51680 NNNGGGAGCTTTTCCTACCA NGG 2 0.1154 Tier III
Download CSV

Other clones with same target sequence:

(none)