Construct: sgRNA BRDN0001148956
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCACCAGCTGAGTAACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST1 (22983)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76124
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
12851971 |
- |
MAST1 |
NNNCACCAGCTGAGTAACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
79207299 |
+ |
MAF |
NNNCACCAGATGAGTAACAA |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000016.10 |
16 |
79207299 |
+ |
WWOX |
NNNCACCAGATGAGTAACAA |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000004.12 |
4 |
186651824 |
- |
FAT1 |
NNNCACCACCTGAGTAAAGA |
NGG |
2 |
0.2899 |
Tier II |
| 5 |
NC_000004.12 |
4 |
9898852 |
- |
SLC2A9 |
NNNCACCAGCTGACTAAAGA |
NGG |
2 |
0.2308 |
Tier II |
| 6 |
NC_000016.10 |
16 |
84137538 |
- |
HSDL1 |
NNNCCCCAGCTGAGTAACCA |
NGG |
2 |
0.2241 |
Tier II |
| 7 |
NC_000011.10 |
11 |
124632904 |
+ |
TBRG1 |
NNNCAGCAGCTGACTAACGA |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000001.11 |
1 |
221729011 |
+ |
DUSP10 |
NNNCACCAGCTGAGTAACAC |
NGG |
2 |
0.1623 |
Tier II |
| 9 |
NC_000012.12 |
12 |
132557177 |
+ |
FBRSL1 |
NNNCACCCGCTGCGTAACGA |
NGG |
2 |
0.0902 |
Tier II |
| 10 |
NC_000010.11 |
10 |
7804536 |
- |
ATP5F1C |
NNNCCCCAGCTGAGTAAGGA |
NGG |
2 |
0.0667 |
Tier II |
| 11 |
NC_000022.11 |
22 |
25092989 |
+ |
KIAA1671 |
NNNCACCAGCTGAGTATGGA |
NGG |
2 |
0.0178 |
Tier II |
| 12 |
NC_000004.12 |
4 |
67038299 |
- |
LOC105377262 |
NNNCATCAGCTGAATAACGA |
NGG |
2 |
0.6964 |
Tier III |
| 13 |
NC_000006.12 |
6 |
43991561 |
- |
LOC124901321 |
NNNCACCAGCTGAGTAACCG |
NGG |
2 |
0.3428 |
Tier III |
| 14 |
NC_000010.11 |
10 |
7804536 |
- |
LOC105376392 |
NNNCCCCAGCTGAGTAAGGA |
NGG |
2 |
0.0667 |
Tier III |
| 15 |
NC_000023.11 |
X |
23211446 |
+ |
PTCHD1-AS |
NNNCACCAGCTGAGGAATGA |
NGG |
2 |
0.0321 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
84925710 |
+ |
Mast1 |
NNNCACCAGCTGAGTGACAA |
NGG |
2 |
0.1374 |
Tier I |
| 2 |
NC_000080.6 |
14 |
103153524 |
- |
Mycbp2 |
NNNAACCAGCTGAGTAACAA |
NGG |
2 |
0.6015 |
Tier II |
| 3 |
NC_000080.6 |
14 |
117790862 |
+ |
Gpc6 |
NNNCACCAGCTGAGGAACAA |
NGG |
2 |
0.0357 |
Tier II |
| 4 |
NC_000069.6 |
3 |
14129764 |
+ |
Ralyl |
NNNCACCAGCTGAGTCACCA |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000069.6 |
3 |
33129621 |
+ |
Pex5l |
NNNCACCAGCTGAGTCAGGA |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000077.6 |
11 |
40055978 |
- |
Gm12132 |
NNNCACCATCTCAGTAACGA |
NGG |
2 |
0.2824 |
Tier III |
Other clones with same target sequence:
(none)
