Construct: sgRNA BRDN0001148961

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAATTGGTGAAACACTACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YES1 (7525)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77967

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000018.10 18 745713 - YES1 NNNTTGGTGAAACACTACAC NGG 0 1.0 Tier I
2 NC_000012.12 12 39533828 - ABCD2 NNNTTGGTAAAACACTACAC NGG 1 0.6429 Tier II
3 NC_000023.11 X 6204796 + NLGN4X NNNTTTGAGAAACACTACAC NGG 2 0.5333 Tier II
4 NC_000009.12 9 125169485 + PPP6C NNNTGGGGGAAACACTACAC NGG 2 0.4693 Tier II
5 NC_000010.11 10 49911461 + PARG NNNTTTGTGAAACACCACAC NGG 2 0.4444 Tier II
6 NC_000003.12 3 11650976 + VGLL4 NNNTTTGTAAAACACTACAC NGG 2 0.4286 Tier II
7 NC_000003.12 3 69318506 + FRMD4B NNNTTGGTGGAACACCACAC NGG 2 0.3704 Tier II
8 NC_000008.11 8 18775935 - PSD3 NNNTTGGGGAAACACTACAA NGG 2 0.3667 Tier II
9 NC_000011.10 11 77230477 + GDPD4 NNNTAGGTGAAACACTACAA NGG 2 0.25 Tier II
10 NC_000023.11 X 130133135 + AIFM1 NNNTTGGTGAAAAACTAAAC NGG 2 0.2071 Tier II
11 NC_000023.11 X 130133135 + RAB33A NNNTTGGTGAAAAACTAAAC NGG 2 0.2071 Tier II
12 NC_000012.12 12 81629808 + PPFIA2 NNNTTGGTGAGCCACTACAC NGG 2 0.1711 Tier II
13 NC_000003.12 3 69463976 + FRMD4B NNNTTGCTGAAACACTACCC NGG 2 0.1422 Tier II
14 NC_000002.12 2 56274650 - CCDC85A NNNTTGGTGAAACACTACTC NAG 2 0.1396 Tier II
15 NC_000009.12 9 35231502 + UNC13B NNNTTGGTGAAAAACTACAT NGG 2 0.1154 Tier II
16 NC_000010.11 10 114191220 - TDRD1 NNNTAGGTGAAAGACTACAC NGG 2 0.0682 Tier II
17 NC_000019.10 19 57389898 - ZNF548 NNNTTGGTGAAACACCACAC NGA 2 0.0463 Tier II
18 NC_000006.12 6 11023905 + ELOVL2 NNNTTGGTGAAACACTATAC NTG 2 0.025 Tier II
19 NC_000007.14 7 110810200 + IMMP2L NNNTTGGTGAAACAGTACAA NGG 2 0.025 Tier II
20 NC_000009.12 9 37156955 - ZCCHC7 NNNTTTGTGAAACACTACAC NGT 2 0.0108 Tier II
21 NC_000002.12 2 40464615 + SLC8A1 NNNTTGGTGAAAGACTACAG NGG 2 0.008 Tier II
22 NC_000008.11 8 76688315 + ZFHX4 NNNTTGGTGAAACACTTCAC NTG 2 0.0052 Tier II
23 NC_000010.11 10 45942372 + PARGP1 NNNTTTGTGAAACACCACAC NGG 2 0.4444 Tier III
24 NC_000010.11 10 45942372 + PARGP1-AGAP4 NNNTTTGTGAAACACCACAC NGG 2 0.4444 Tier III
25 NC_000013.11 13 62723175 + LINC00448 NNNTTGGTCAAATACTACAC NGG 2 0.3769 Tier III
26 NC_000015.10 15 93154260 - LOC101927025 NNNTTGGTCAAACACTACGC NGG 2 0.2019 Tier III
27 NC_000008.11 8 125469259 - TRIB1AL NNNTTGGTGAAACCCTTCAC NGG 2 0.0286 Tier III
28 NC_000016.10 16 86283166 + LINC01081 NNNTTTGTGAAACACTACAC NGC 2 0.0148 Tier III
29 NC_000023.11 X 39243640 - LOC105373175 NNNTTGGTGAAACACTTCAC NGT 2 0.0022 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 32653302 + Yes1 NNNCTGGTGAAGCACTACAC NGG 2 0.4673 Tier I
2 NC_000068.7 2 37466219 - Rabgap1 NNNTTATTGAAACACTACAC NGG 2 0.5714 Tier II
3 NC_000084.6 18 9372131 + Ccny NNNTTGGTGAAATACTACAT NGG 2 0.21 Tier II
4 NC_000077.6 11 45831643 - F630206G17Rik NNNTCTGTGAAACACTACAC NGG 2 0.6667 Tier III
5 NC_000084.6 18 22339753 + Gm41676 NNNTTGTTGAAATACTACAC NGG 2 0.4 Tier III
6 NC_000078.6 12 109621610 + Rian NNNTTTGTGAATCACTACAC NGG 2 0.2222 Tier III
7 NC_000073.6 7 84758808 + 2610206C17Rik NNNTTGGTGAAACAGTACCC NGG 2 0.0103 Tier III
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Other clones with same target sequence:

(none)