Construct: sgRNA BRDN0001148963
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGGGAGCGGACGGTAGCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TKFC (26007)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75785
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
61342827 |
- |
TKFC |
NNNGGAGCGGACGGTAGCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
130286959 |
- |
HMCN2 |
NNNGGAGCGGACGGGAGTGT |
NGG |
2 |
0.0321 |
Tier II |
| 3 |
NC_000011.10 |
11 |
45722435 |
+ |
LOC100507384 |
NNNGGAGGGGACGGTAGCGT |
NAG |
2 |
0.1667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
26962972 |
+ |
Syngap1 |
NNNGGAGCGGACCGTAGCCT |
NGG |
2 |
0.1887 |
Tier I |
| 2 |
NC_000081.6 |
15 |
97996432 |
+ |
Col2a1 |
NNNGGAGCAGACGGTAGCTT |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000067.6 |
1 |
93765377 |
+ |
Atg4b |
NNNGGAGCGGAGAGTAGCGT |
NGG |
2 |
0.4103 |
Tier II |
| 4 |
NC_000076.6 |
10 |
60123694 |
+ |
Spock2 |
NNNGGAGAGGACGGGAGCGT |
NGG |
2 |
0.0325 |
Tier II |
| 5 |
NC_000081.6 |
15 |
97996432 |
+ |
Gm46536 |
NNNGGAGCAGACGGTAGCTT |
NGG |
2 |
0.4286 |
Tier III |
Other clones with same target sequence:
(none)
