Construct: sgRNA BRDN0001148967
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCAACGTAACGTGAGCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKB (5257)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77845
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
47610903 |
+ |
PHKB |
NNNAACGTAACGTGAGCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
77350969 |
+ |
PDE8B |
NNNAATGTAACGAGAGCATG |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000003.12 |
3 |
132332150 |
- |
ACP3 |
NNNAACGTAAAGGGAGCATG |
NGG |
2 |
0.1957 |
Tier II |
| 4 |
NC_000022.11 |
22 |
32723251 |
+ |
SYN3 |
NNNAAGGTAACGTGAGCATG |
NGC |
2 |
0.0111 |
Tier II |
| 5 |
NC_000014.9 |
14 |
65523042 |
+ |
FUT8 |
NNNAACGTAACGTGTGCATG |
NGT |
2 |
0.0032 |
Tier II |
| 6 |
NC_000024.10 |
Y |
22109790 |
+ |
LOC102725532 |
NNNAATGAAACGTGAGCATG |
NGG |
2 |
0.7429 |
Tier III |
| 7 |
NC_000024.10 |
Y |
22476617 |
- |
LOC101929148 |
NNNAATGAAACGTGAGCATG |
NGG |
2 |
0.7429 |
Tier III |
| 8 |
NC_000017.11 |
17 |
77811568 |
- |
LOC105371908 |
NNNAATGTAAGGTGAGCATG |
NGG |
2 |
0.2321 |
Tier III |
| 9 |
NC_000010.11 |
10 |
120174955 |
+ |
LOC105378515 |
NNNAACGTCACTTGAGCATG |
NGG |
2 |
0.2198 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
85970980 |
+ |
Phkb |
NNNAACGTAACGTGAGCATG |
NGA |
1 |
0.0694 |
Tier I |
| 2 |
NC_000085.6 |
19 |
34730965 |
+ |
Slc16a12 |
NNNAATGTAAAGTGAGCATG |
NGG |
2 |
0.6964 |
Tier II |
| 3 |
NC_000069.6 |
3 |
146587313 |
+ |
Dnase2b |
NNNAACATACCGTGAGCATG |
NGG |
2 |
0.3333 |
Tier II |
Other clones with same target sequence:
(none)
