Construct: sgRNA BRDN0001148972
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGACAGAGTACATTGAGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LIMK2 (3985)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75974
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000022.11 |
22 |
31267874 |
+ |
LIMK2 |
NNNACAGAGTACATTGAGGG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000012.12 |
12 |
1375698 |
+ |
ERC1 |
NNNACACAGTACATTGAGGG |
NGG |
1 |
0.6875 |
Tier II |
3 |
NC_000002.12 |
2 |
58026096 |
- |
VRK2 |
NNNACAGAGTACATTGAAAG |
NGG |
2 |
0.4945 |
Tier II |
4 |
NC_000011.10 |
11 |
103305635 |
+ |
DYNC2H1 |
NNNATAGAGTAAATTGAGGG |
NGG |
2 |
0.4545 |
Tier II |
5 |
NC_000011.10 |
11 |
58113483 |
- |
OR9Q1 |
NNNAGAGAGTACAATGAGGG |
NGG |
2 |
0.3714 |
Tier II |
6 |
NC_000014.9 |
14 |
50212698 |
+ |
SOS2 |
NNNACAGAGGAAATTGAGGG |
NGG |
2 |
0.3571 |
Tier II |
7 |
NC_000014.9 |
14 |
35782724 |
- |
RALGAPA1 |
NNNAAAGAGTACATTGAGGC |
NGG |
2 |
0.2449 |
Tier II |
8 |
NC_000016.10 |
16 |
56275124 |
+ |
GNAO1 |
NNNACATAGTTCATTGAGGG |
NGG |
2 |
0.1758 |
Tier II |
9 |
NC_000006.12 |
6 |
65319763 |
- |
EYS |
NNNACAGACTACTTTGAGGG |
NGG |
2 |
0.1615 |
Tier II |
10 |
NC_000002.12 |
2 |
140556325 |
- |
LRP1B |
NNNATAGAGTACATTGTGGG |
NGG |
2 |
0.0848 |
Tier II |
11 |
NC_000006.12 |
6 |
64875107 |
- |
EYS |
NNNAGAGAGTACATGGAGGG |
NGG |
2 |
0.03 |
Tier II |
12 |
NC_000005.10 |
5 |
84085806 |
- |
EDIL3 |
NNNACAGAGCACATGGAGGG |
NGG |
2 |
0.0267 |
Tier II |
13 |
NC_000012.12 |
12 |
122997868 |
+ |
PITPNM2 |
NNNACAGAGGACATGGAGGG |
NGG |
2 |
0.025 |
Tier II |
14 |
NC_000012.12 |
12 |
98892422 |
- |
ANKS1B |
NNNACAGAGTACATTGTGGG |
NGA |
2 |
0.0093 |
Tier II |
15 |
NC_000018.10 |
18 |
7786710 |
- |
PTPRM |
NNNACAGAGTACAATTAGGG |
NGG |
2 |
0.0 |
Tier II |
16 |
NC_000003.12 |
3 |
127916306 |
+ |
KBTBD12 |
NNNACAGAGTACATTTAGGG |
NGG |
1 |
0.0 |
Tier II |
17 |
NC_000014.9 |
14 |
46078449 |
- |
LINC00871 |
NNNATGGAGTACATTGAGGG |
NGG |
2 |
0.4545 |
Tier III |
18 |
NC_000003.12 |
3 |
157220369 |
+ |
LOC101928236 |
NNNAGAGAGTACCTTGAGGG |
NGG |
2 |
0.1263 |
Tier III |
19 |
NC_000001.11 |
1 |
58762706 |
+ |
LOC105378751 |
NNNACAGAGTACATGGGGGG |
NGG |
2 |
0.0088 |
Tier III |
20 |
NC_000017.11 |
17 |
36120302 |
+ |
LOC101927369 |
NNNACAGAGCACAGTGAGGG |
NGG |
2 |
0.0 |
Tier III |
21 |
NC_000003.12 |
3 |
127916306 |
+ |
LOC107986129 |
NNNACAGAGTACATTTAGGG |
NGG |
1 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000071.6 |
5 |
134664219 |
- |
Limk1 |
NNNACAGAGTACATCAAGGG |
NGG |
2 |
0.2727 |
Tier I |
2 |
NC_000077.6 |
11 |
3350676 |
- |
Limk2 |
NNNACCGAGTACATCGAGGG |
NGG |
2 |
0.124 |
Tier I |
3 |
NC_000076.6 |
10 |
80254611 |
+ |
Ndufs7 |
NNNACAGAGAACATTGAGGG |
NGG |
1 |
0.8571 |
Tier II |
4 |
NC_000083.6 |
17 |
7294867 |
+ |
Rps6ka2 |
NNNACAGAGTGCATTGAGTG |
NGG |
2 |
0.4333 |
Tier II |
5 |
NC_000068.7 |
2 |
62140026 |
- |
Slc4a10 |
NNNACAGATTAAATTGAGGG |
NGG |
2 |
0.381 |
Tier II |
6 |
NC_000072.6 |
6 |
25776739 |
- |
Pot1a |
NNNACAGAGTACGTAGAGGG |
NGG |
2 |
0.3776 |
Tier II |
7 |
NC_000075.6 |
9 |
56786392 |
+ |
Lingo1 |
NNNACAGCCTACATTGAGGG |
NGG |
2 |
0.2308 |
Tier II |
8 |
NC_000075.6 |
9 |
86309319 |
+ |
Ube2cbp |
NNNACAGAGTCTATTGAGGG |
NGG |
2 |
0.2154 |
Tier II |
9 |
NC_000084.6 |
18 |
75556424 |
- |
Ctif |
NNNACTGAGTACACTGAGGG |
NGG |
2 |
0.2041 |
Tier II |
10 |
NC_000068.7 |
2 |
153779968 |
- |
Efcab8 |
NNNACAGAGTACCTTGAGGA |
NGG |
2 |
0.1974 |
Tier II |
11 |
NC_000072.6 |
6 |
135230573 |
+ |
Fam234b |
NNNACAGAGTCCATTGAGGC |
NGG |
2 |
0.1714 |
Tier II |
12 |
NC_000069.6 |
3 |
27445925 |
- |
Fndc3b |
NNNACAGAGTACATTGCGGA |
NGG |
2 |
0.1654 |
Tier II |
13 |
NC_000071.6 |
5 |
142437413 |
+ |
Foxk1 |
NNNATAGAGTACATTGTGGG |
NGG |
2 |
0.0848 |
Tier II |
14 |
NC_000076.6 |
10 |
36724952 |
+ |
Hs3st5 |
NNNACAAAGTACATGGAGGG |
NGG |
2 |
0.05 |
Tier II |
15 |
NC_000084.6 |
18 |
6038809 |
+ |
Arhgap12 |
NNNACAGAGTACATGGAGGG |
NGG |
1 |
0.05 |
Tier II |
16 |
NC_000071.6 |
5 |
86418972 |
- |
Tmprss11a |
NNNACAGAGTACATTGTGGG |
NAG |
2 |
0.0346 |
Tier II |
17 |
NC_000067.6 |
1 |
132455014 |
+ |
Dstyk |
NNNACAGAATACATTGAGGG |
NTG |
2 |
0.025 |
Tier II |
18 |
NC_000074.6 |
8 |
117410571 |
- |
Cmip |
NNNACAGAGTCCATGGAGGG |
NGG |
2 |
0.02 |
Tier II |
19 |
NC_000075.6 |
9 |
111335144 |
- |
Trank1 |
NNNACAGAGAACAGTGAGGG |
NGG |
2 |
0.0 |
Tier II |
20 |
NC_000070.6 |
4 |
106489322 |
+ |
Bsnd |
NNNACAGAGTACAGTGAGGG |
NGG |
1 |
0.0 |
Tier II |
21 |
NC_000082.6 |
16 |
25803841 |
+ |
Trp63 |
NNNACAGAGTACAGTGAGGG |
NGT |
2 |
0.0 |
Tier II |
22 |
NC_000081.6 |
15 |
97348540 |
+ |
Pced1b |
NNNACAGAGTACATTCAGGG |
NTG |
2 |
0.0 |
Tier II |
23 |
NC_000076.6 |
10 |
118232434 |
- |
Gm9585 |
NNNACAGAGTACATTAACGG |
NGG |
2 |
0.4762 |
Tier III |
24 |
NC_000076.6 |
10 |
118267035 |
+ |
Gm9035 |
NNNACAGAGTACATTAACGG |
NGG |
2 |
0.4762 |
Tier III |
25 |
NC_000086.7 |
X |
105168734 |
- |
Gm33605 |
NNNACAGAGTGCAATGAGGG |
NGG |
2 |
0.4024 |
Tier III |
26 |
NC_000083.6 |
17 |
29449736 |
+ |
Gm36199 |
NNNACAGAGTAGATTGTGGG |
NGG |
2 |
0.0593 |
Tier III |
27 |
NC_000083.6 |
17 |
29449736 |
+ |
Gm26885 |
NNNACAGAGTAGATTGTGGG |
NGG |
2 |
0.0593 |
Tier III |
28 |
NC_000081.6 |
15 |
97348540 |
+ |
Gm33325 |
NNNACAGAGTACATTCAGGG |
NTG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)