Construct: sgRNA BRDN0001148972

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGACAGAGTACATTGAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIMK2 (3985)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75974

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000022.11 22 31267874 + LIMK2 NNNACAGAGTACATTGAGGG NGG 0 1.0 Tier I
2 NC_000012.12 12 1375698 + ERC1 NNNACACAGTACATTGAGGG NGG 1 0.6875 Tier II
3 NC_000002.12 2 58026096 - VRK2 NNNACAGAGTACATTGAAAG NGG 2 0.4945 Tier II
4 NC_000011.10 11 103305635 + DYNC2H1 NNNATAGAGTAAATTGAGGG NGG 2 0.4545 Tier II
5 NC_000011.10 11 58113483 - OR9Q1 NNNAGAGAGTACAATGAGGG NGG 2 0.3714 Tier II
6 NC_000014.9 14 50212698 + SOS2 NNNACAGAGGAAATTGAGGG NGG 2 0.3571 Tier II
7 NC_000014.9 14 35782724 - RALGAPA1 NNNAAAGAGTACATTGAGGC NGG 2 0.2449 Tier II
8 NC_000016.10 16 56275124 + GNAO1 NNNACATAGTTCATTGAGGG NGG 2 0.1758 Tier II
9 NC_000006.12 6 65319763 - EYS NNNACAGACTACTTTGAGGG NGG 2 0.1615 Tier II
10 NC_000002.12 2 140556325 - LRP1B NNNATAGAGTACATTGTGGG NGG 2 0.0848 Tier II
11 NC_000006.12 6 64875107 - EYS NNNAGAGAGTACATGGAGGG NGG 2 0.03 Tier II
12 NC_000005.10 5 84085806 - EDIL3 NNNACAGAGCACATGGAGGG NGG 2 0.0267 Tier II
13 NC_000012.12 12 122997868 + PITPNM2 NNNACAGAGGACATGGAGGG NGG 2 0.025 Tier II
14 NC_000012.12 12 98892422 - ANKS1B NNNACAGAGTACATTGTGGG NGA 2 0.0093 Tier II
15 NC_000018.10 18 7786710 - PTPRM NNNACAGAGTACAATTAGGG NGG 2 0.0 Tier II
16 NC_000003.12 3 127916306 + KBTBD12 NNNACAGAGTACATTTAGGG NGG 1 0.0 Tier II
17 NC_000014.9 14 46078449 - LINC00871 NNNATGGAGTACATTGAGGG NGG 2 0.4545 Tier III
18 NC_000003.12 3 157220369 + LOC101928236 NNNAGAGAGTACCTTGAGGG NGG 2 0.1263 Tier III
19 NC_000001.11 1 58762706 + LOC105378751 NNNACAGAGTACATGGGGGG NGG 2 0.0088 Tier III
20 NC_000017.11 17 36120302 + LOC101927369 NNNACAGAGCACAGTGAGGG NGG 2 0.0 Tier III
21 NC_000003.12 3 127916306 + LOC107986129 NNNACAGAGTACATTTAGGG NGG 1 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 134664219 - Limk1 NNNACAGAGTACATCAAGGG NGG 2 0.2727 Tier I
2 NC_000077.6 11 3350676 - Limk2 NNNACCGAGTACATCGAGGG NGG 2 0.124 Tier I
3 NC_000076.6 10 80254611 + Ndufs7 NNNACAGAGAACATTGAGGG NGG 1 0.8571 Tier II
4 NC_000083.6 17 7294867 + Rps6ka2 NNNACAGAGTGCATTGAGTG NGG 2 0.4333 Tier II
5 NC_000068.7 2 62140026 - Slc4a10 NNNACAGATTAAATTGAGGG NGG 2 0.381 Tier II
6 NC_000072.6 6 25776739 - Pot1a NNNACAGAGTACGTAGAGGG NGG 2 0.3776 Tier II
7 NC_000075.6 9 56786392 + Lingo1 NNNACAGCCTACATTGAGGG NGG 2 0.2308 Tier II
8 NC_000075.6 9 86309319 + Ube2cbp NNNACAGAGTCTATTGAGGG NGG 2 0.2154 Tier II
9 NC_000084.6 18 75556424 - Ctif NNNACTGAGTACACTGAGGG NGG 2 0.2041 Tier II
10 NC_000068.7 2 153779968 - Efcab8 NNNACAGAGTACCTTGAGGA NGG 2 0.1974 Tier II
11 NC_000072.6 6 135230573 + Fam234b NNNACAGAGTCCATTGAGGC NGG 2 0.1714 Tier II
12 NC_000069.6 3 27445925 - Fndc3b NNNACAGAGTACATTGCGGA NGG 2 0.1654 Tier II
13 NC_000071.6 5 142437413 + Foxk1 NNNATAGAGTACATTGTGGG NGG 2 0.0848 Tier II
14 NC_000076.6 10 36724952 + Hs3st5 NNNACAAAGTACATGGAGGG NGG 2 0.05 Tier II
15 NC_000084.6 18 6038809 + Arhgap12 NNNACAGAGTACATGGAGGG NGG 1 0.05 Tier II
16 NC_000071.6 5 86418972 - Tmprss11a NNNACAGAGTACATTGTGGG NAG 2 0.0346 Tier II
17 NC_000067.6 1 132455014 + Dstyk NNNACAGAATACATTGAGGG NTG 2 0.025 Tier II
18 NC_000074.6 8 117410571 - Cmip NNNACAGAGTCCATGGAGGG NGG 2 0.02 Tier II
19 NC_000075.6 9 111335144 - Trank1 NNNACAGAGAACAGTGAGGG NGG 2 0.0 Tier II
20 NC_000070.6 4 106489322 + Bsnd NNNACAGAGTACAGTGAGGG NGG 1 0.0 Tier II
21 NC_000082.6 16 25803841 + Trp63 NNNACAGAGTACAGTGAGGG NGT 2 0.0 Tier II
22 NC_000081.6 15 97348540 + Pced1b NNNACAGAGTACATTCAGGG NTG 2 0.0 Tier II
23 NC_000076.6 10 118232434 - Gm9585 NNNACAGAGTACATTAACGG NGG 2 0.4762 Tier III
24 NC_000076.6 10 118267035 + Gm9035 NNNACAGAGTACATTAACGG NGG 2 0.4762 Tier III
25 NC_000086.7 X 105168734 - Gm33605 NNNACAGAGTGCAATGAGGG NGG 2 0.4024 Tier III
26 NC_000083.6 17 29449736 + Gm36199 NNNACAGAGTAGATTGTGGG NGG 2 0.0593 Tier III
27 NC_000083.6 17 29449736 + Gm26885 NNNACAGAGTAGATTGTGGG NGG 2 0.0593 Tier III
28 NC_000081.6 15 97348540 + Gm33325 NNNACAGAGTACATTCAGGG NTG 2 0.0 Tier III
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Other clones with same target sequence:

(none)