Construct: sgRNA BRDN0001148973
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGACCACTCTAAATTTGACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TEK (7010)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75724
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
27197374 |
+ |
TEK |
NNNCCACTCTAAATTTGACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
74619703 |
- |
CLK3 |
NNNCAACTCTAAGTTTGACC |
NGG |
2 |
0.3727 |
Tier II |
| 3 |
NC_000004.12 |
4 |
89036406 |
+ |
FAM13A |
NNNCCACTCGAGATTTGACC |
NGG |
2 |
0.3611 |
Tier II |
| 4 |
NC_000006.12 |
6 |
63974385 |
- |
EYS |
NNNGCACTCTAAATATGACC |
NGG |
2 |
0.2895 |
Tier II |
| 5 |
NC_000003.12 |
3 |
51620633 |
- |
RAD54L2 |
NNNCCACTATAAATTTGACT |
NGG |
2 |
0.2571 |
Tier II |
| 6 |
NC_000020.11 |
20 |
25050540 |
+ |
ACSS1 |
NNNCCACTCCAAATTTGACC |
NTG |
2 |
0.0208 |
Tier II |
| 7 |
NC_000006.12 |
6 |
63974385 |
- |
LOC107986608 |
NNNGCACTCTAAATATGACC |
NGG |
2 |
0.2895 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
67583285 |
+ |
Tln2 |
NNNCCACTCTAAATTTATCC |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000073.6 |
7 |
99324522 |
+ |
Map6 |
NNNCCACTCTGAATTTGAGC |
NGG |
2 |
0.0813 |
Tier II |
| 3 |
NC_000086.7 |
X |
71054202 |
+ |
Mamld1 |
NNNCCACTCTAAATTTGACC |
NGC |
1 |
0.0222 |
Tier II |
Other clones with same target sequence:
(none)
