Construct: sgRNA BRDN0001148974

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAATACAAGTGGTCAACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPNE3 (8895)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77552

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 86528666 + CPNE3 NNNATACAAGTGGTCAACAG NGG 0 1.0 Tier I
2 NC_000002.12 2 171031260 - TLK1 NNNATACAAATGGACAACAG NGG 2 0.5778 Tier II
3 NC_000005.10 5 26953768 - CDH9 NNNATTCAGGTGGTCAACAG NGG 2 0.4762 Tier II
4 NC_000002.12 2 27298113 - TRIM54 NNNATACAAGTGCTCAACAA NGG 2 0.3947 Tier II
5 NC_000006.12 6 16316839 + ATXN1 NNNGTACAAGTGGACAACAG NGG 2 0.3869 Tier II
6 NC_000007.14 7 102297058 + SH2B2 NNNATACCCGTGGTCAACAG NGG 2 0.2449 Tier II
7 NC_000002.12 2 182843991 - FRZB NNNATACAAGTGGTCAACAA NAG 2 0.2431 Tier II
8 NC_000015.10 15 31745920 + OTUD7A NNNATACAAATGGTCAACAG NAG 2 0.242 Tier II
9 NC_000010.11 10 119928631 - SEC23IP NNNATCCAAGTGTTCAACAG NGG 2 0.1364 Tier II
10 NC_000001.11 1 103053702 + COL11A1 NNNATACAAGTGGTCAGTAG NGG 2 0.1134 Tier II
11 NC_000006.12 6 108500935 - AFG1L NNNATACAAGTGGTCAACAA NGA 2 0.0651 Tier II
12 NC_000006.12 6 35314546 - DEF6 NNNATACAAATGGTCAACAG NGA 2 0.0648 Tier II
13 NC_000019.10 19 55922667 + NLRP13 NNNATACAATTGGTTAACAG NGG 2 0.0542 Tier II
14 NC_000003.12 3 155134916 - MME NNNATACAAGTGGTCAACAA NTG 2 0.0365 Tier II
15 NC_000023.11 X 112197192 + RTL4 NNNATACAAATGGTCAACAG NTG 2 0.0364 Tier II
16 NC_000006.12 6 57519966 - PRIM2 NNNATACAAGTGGTCAACAT NTG 2 0.0273 Tier II
17 NC_000002.12 2 233781517 - MROH2A NNNATACAAGTGGCCAACAG NGA 2 0.0198 Tier II
18 NC_000022.11 22 45777467 + ATXN10 NNNATACAAGTGGTAAACAG NTG 2 0.0087 Tier II
19 NC_000003.12 3 37928511 - CTDSPL NNNATACAAGTGGTCAACAG NTA 2 0.0 Tier II
20 NC_000006.12 6 44441740 - CDC5L NNNATACAAGTGGTCAACAG NTA 2 0.0 Tier II
21 NC_000012.12 12 39680362 + REDIC1 NNNATACAAGTGGTCAACAG NTA 2 0.0 Tier II
22 NC_000012.12 12 86618063 + MGAT4C NNNATACAAGTGGTCAACAG NTA 2 0.0 Tier II
23 NC_000017.11 17 2943764 + RAP1GAP2 NNNATACAAGTGGTCAACAG NTA 2 0.0 Tier II
24 NC_000003.12 3 21091365 + LOC105376987 NNNAGACAAATGGTCAACAG NGG 2 0.5973 Tier III
25 NC_000003.12 3 135831903 - LOC105374124 NNNATTGAAGTGGTCAACAG NGG 2 0.3361 Tier III
26 NC_000007.14 7 94297292 + LOC112267858 NNNATAAAAGTGGTCAACAC NGG 2 0.3214 Tier III
27 NC_000006.12 6 155861660 - LOC101928923 NNNATACAAGTGGCCAACAG NCG 2 0.0306 Tier III
28 NC_000006.12 6 155861660 - LOC105378072 NNNATACAAGTGGCCAACAG NCG 2 0.0306 Tier III
29 NC_000003.12 3 91501366 + LOC101930420 NNNATACAAGTGGTCAACAT NTG 2 0.0273 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 66262712 - Lrrk1 NNNATGGAAGTGGTCAACAG NGG 2 0.3361 Tier I
2 NC_000070.6 4 19555374 - Cpne3 NNNATACGAGTGGTCACCAG NGG 2 0.1294 Tier I
3 NC_000067.6 1 179503555 - Smyd3 NNNATACAAGAGGTTAACAG NGG 2 0.05 Tier II
4 NC_000072.6 6 22335499 + Fam3c NNNATACAAGTGATCAACAG NTG 2 0.036 Tier II
5 NC_000082.6 16 9858675 - Grin2a NNNATACAAGTGGTCAGGAG NGG 2 0.0235 Tier II
6 NC_000080.6 14 39208995 + Nrg3 NNNATAAAAGTGGTCAACAG NGT 2 0.0121 Tier II
7 NC_000071.6 5 123125565 - Rhof NNNATACAAGTGGTAAACAG NGT 2 0.0036 Tier II
8 NC_000070.6 4 139431302 + Ubr4 NNNATACAAGTGGGCACCAG NGG 2 0.0 Tier II
9 NC_000086.7 X 162080179 + Nhs NNNATACAAGTGGGCAGCAG NGG 2 0.0 Tier II
10 NC_000072.6 6 66198277 - Gm36408 NNNATACAAATAGTCAACAG NGG 2 0.8711 Tier III
11 NC_000073.6 7 66262712 - Gm33442 NNNATGGAAGTGGTCAACAG NGG 2 0.3361 Tier III
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Other clones with same target sequence:

(none)