GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001148974

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: TGAATACAAGTGGTCAACAG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: CPNE3 (8895)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 77552

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000008.11	8	86528666	+	CPNE3	NNNATACAAGTGGTCAACAG	NGG	0	1.0	Tier I
2	NC_000002.12	2	171031260	-	TLK1	NNNATACAAATGGACAACAG	NGG	2	0.5778	Tier II
3	NC_000005.10	5	26953768	-	CDH9	NNNATTCAGGTGGTCAACAG	NGG	2	0.4762	Tier II
4	NC_000002.12	2	27298113	-	TRIM54	NNNATACAAGTGCTCAACAA	NGG	2	0.3947	Tier II
5	NC_000006.12	6	16316839	+	ATXN1	NNNGTACAAGTGGACAACAG	NGG	2	0.3869	Tier II
6	NC_000007.14	7	102297058	+	SH2B2	NNNATACCCGTGGTCAACAG	NGG	2	0.2449	Tier II
7	NC_000002.12	2	182843991	-	FRZB	NNNATACAAGTGGTCAACAA	NAG	2	0.2431	Tier II
8	NC_000015.10	15	31745920	+	OTUD7A	NNNATACAAATGGTCAACAG	NAG	2	0.242	Tier II
9	NC_000010.11	10	119928631	-	SEC23IP	NNNATCCAAGTGTTCAACAG	NGG	2	0.1364	Tier II
10	NC_000001.11	1	103053702	+	COL11A1	NNNATACAAGTGGTCAGTAG	NGG	2	0.1134	Tier II
11	NC_000006.12	6	108500935	-	AFG1L	NNNATACAAGTGGTCAACAA	NGA	2	0.0651	Tier II
12	NC_000006.12	6	35314546	-	DEF6	NNNATACAAATGGTCAACAG	NGA	2	0.0648	Tier II
13	NC_000019.10	19	55922667	+	NLRP13	NNNATACAATTGGTTAACAG	NGG	2	0.0542	Tier II
14	NC_000003.12	3	155134916	-	MME	NNNATACAAGTGGTCAACAA	NTG	2	0.0365	Tier II
15	NC_000023.11	X	112197192	+	RTL4	NNNATACAAATGGTCAACAG	NTG	2	0.0364	Tier II
16	NC_000006.12	6	57519966	-	PRIM2	NNNATACAAGTGGTCAACAT	NTG	2	0.0273	Tier II
17	NC_000002.12	2	233781517	-	MROH2A	NNNATACAAGTGGCCAACAG	NGA	2	0.0198	Tier II
18	NC_000022.11	22	45777467	+	ATXN10	NNNATACAAGTGGTAAACAG	NTG	2	0.0087	Tier II
19	NC_000003.12	3	37928511	-	CTDSPL	NNNATACAAGTGGTCAACAG	NTA	2	0.0	Tier II
20	NC_000006.12	6	44441740	-	CDC5L	NNNATACAAGTGGTCAACAG	NTA	2	0.0	Tier II
21	NC_000006.12	6	44441740	-	POLR1C	NNNATACAAGTGGTCAACAG	NTA	2	0.0	Tier II
22	NC_000012.12	12	39680362	+	REDIC1	NNNATACAAGTGGTCAACAG	NTA	2	0.0	Tier II
23	NC_000012.12	12	86618063	+	MGAT4C	NNNATACAAGTGGTCAACAG	NTA	2	0.0	Tier II
24	NC_000017.11	17	2943764	+	RAP1GAP2	NNNATACAAGTGGTCAACAG	NTA	2	0.0	Tier II
25	NC_000003.12	3	21091365	+	LOC105376987	NNNAGACAAATGGTCAACAG	NGG	2	0.5973	Tier III
26	NC_000003.12	3	135831903	-	LOC105374124	NNNATTGAAGTGGTCAACAG	NGG	2	0.3361	Tier III
27	NC_000007.14	7	94297292	+	LOC112267858	NNNATAAAAGTGGTCAACAC	NGG	2	0.3214	Tier III
28	NC_000006.12	6	155861660	-	LOC101928923	NNNATACAAGTGGCCAACAG	NCG	2	0.0306	Tier III
29	NC_000006.12	6	155861660	-	LOC105378072	NNNATACAAGTGGCCAACAG	NCG	2	0.0306	Tier III
30	NC_000003.12	3	91501366	+	LOC101930420	NNNATACAAGTGGTCAACAT	NTG	2	0.0273	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000073.6	7	66262712	-	Lrrk1	NNNATGGAAGTGGTCAACAG	NGG	2	0.3361	Tier I
2	NC_000070.6	4	19555374	-	Cpne3	NNNATACGAGTGGTCACCAG	NGG	2	0.1294	Tier I
3	NC_000067.6	1	179503555	-	Smyd3	NNNATACAAGAGGTTAACAG	NGG	2	0.05	Tier II
4	NC_000072.6	6	22335499	+	Fam3c	NNNATACAAGTGATCAACAG	NTG	2	0.036	Tier II
5	NC_000082.6	16	9858675	-	Grin2a	NNNATACAAGTGGTCAGGAG	NGG	2	0.0235	Tier II
6	NC_000080.6	14	39208995	+	Nrg3	NNNATAAAAGTGGTCAACAG	NGT	2	0.0121	Tier II
7	NC_000071.6	5	123125565	-	Rhof	NNNATACAAGTGGTAAACAG	NGT	2	0.0036	Tier II
8	NC_000070.6	4	139431302	+	Ubr4	NNNATACAAGTGGGCACCAG	NGG	2	0.0	Tier II
9	NC_000086.7	X	162080179	+	Nhs	NNNATACAAGTGGGCAGCAG	NGG	2	0.0	Tier II
10	NC_000072.6	6	66198277	-	Gm36408	NNNATACAAATAGTCAACAG	NGG	2	0.8711	Tier III
11	NC_000073.6	7	66262712	-	Gm33442	NNNATGGAAGTGGTCAACAG	NGG	2	0.3361	Tier III

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Other clones with same target sequence:

(none)