Construct: sgRNA BRDN0001148976
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGAGGAAGTCGAAGTCCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SGK2 (10110)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76207
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
43567678 |
- |
SGK2 |
NNNAGGAAGTCGAAGTCCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
76225177 |
+ |
PCSK5 |
NNNAGGAAGTAGAAGTCCCT |
NGG |
2 |
0.3362 |
Tier II |
| 3 |
NC_000017.11 |
17 |
79777582 |
- |
CBX2 |
NNNAGGAAGACGAAGTCCGG |
NGG |
2 |
0.1513 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
80097196 |
+ |
Lrrtm4 |
NNNAGGAAGTAGAAGTCCTT |
NGG |
2 |
0.5 |
Tier II |
| 2 |
NC_000075.6 |
9 |
57561526 |
+ |
Scamp2 |
NNNAGGAAGTAGAAGTCTGT |
NGG |
2 |
0.4821 |
Tier II |
| 3 |
NC_000083.6 |
17 |
5135876 |
- |
Arid1b |
NNNAGGAAGTCCAAGTCTGT |
NGG |
2 |
0.3403 |
Tier II |
| 4 |
NC_000076.6 |
10 |
101755611 |
- |
Mgat4c |
NNNAGGAAGTGGAAGTCAGT |
NGG |
2 |
0.1346 |
Tier II |
| 5 |
NC_000068.7 |
2 |
42601470 |
- |
Lrp1b |
NNNAGGAAGTGGAAGTCGGT |
NGG |
2 |
0.0333 |
Tier II |
Other clones with same target sequence:
(none)
