Construct: sgRNA BRDN0001148982
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCATCGCAAGTATGACCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP4K2B (8396)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77904
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
38779388 |
- |
PIP4K2B |
NNNTCGCAAGTATGACCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
158734245 |
- |
ESYT2 |
NNNTCTCAGGTATGACCTCA |
NGG |
2 |
0.4444 |
Tier I |
| 3 |
NC_000005.10 |
5 |
98772586 |
- |
RGMB |
NNNTCTCAAGAATGACCTCA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000008.11 |
8 |
70774045 |
- |
XKR9 |
NNNTCTGAAGTATGACCTCA |
NGG |
2 |
0.3137 |
Tier II |
| 5 |
NC_000005.10 |
5 |
98772586 |
- |
RGMB-AS1 |
NNNTCTCAAGAATGACCTCA |
NGG |
2 |
0.5 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
127201044 |
- |
Pip4k2c |
NNNTAGGAAGTATGACCTCA |
NGG |
2 |
0.2689 |
Tier I |
| 2 |
NC_000084.6 |
18 |
65087930 |
- |
Nedd4l |
NNNTCTCAAGCATGACCTCA |
NGG |
2 |
0.4444 |
Tier II |
| 3 |
NC_000067.6 |
1 |
129728744 |
- |
Thsd7b |
NNNACTCAAGTATGACCTCA |
NGG |
2 |
0.3175 |
Tier II |
| 4 |
NC_000078.6 |
12 |
93250593 |
+ |
Gm34869 |
NNNTTGCAAGTATGAACTCA |
NGG |
2 |
0.6364 |
Tier III |
| 5 |
NC_000076.6 |
10 |
127201044 |
- |
F420014N23Rik |
NNNTAGGAAGTATGACCTCA |
NGG |
2 |
0.2689 |
Tier III |
| 6 |
NC_000081.6 |
15 |
102770782 |
- |
Gm36560 |
NNNTCTCAAGTCTGACCTCA |
NGG |
2 |
0.1754 |
Tier III |
Other clones with same target sequence:
(none)
