Construct: sgRNA BRDN0001148988

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGTCCGCTGTTACTCAGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK5 (1020)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77544

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 151055537 - CDK5 NNNCCGCTGTTACTCAGCTG NGG 0 1.0 Tier I
2 NC_000015.10 15 67098838 + SMAD3 NNNCCACTGTTGCTCAGCTG NGG 2 0.7222 Tier II
3 NC_000007.14 7 133050315 - CHCHD3 NNNCCTCTGTTACTCAGCTT NGG 2 0.4667 Tier II
4 NC_000004.12 4 80686595 + CFAP299 NNNCCGCTGTTAATCAACTG NGG 2 0.359 Tier II
5 NC_000010.11 10 106878227 + SORCS1 NNNCCACTGTTTCTCAGCTG NGG 2 0.3333 Tier II
6 NC_000001.11 1 209769536 - TRAF3IP3 NNNCCTGTGTTACTCAGCTG NGG 2 0.3137 Tier II
7 NC_000006.12 6 16524519 - ATXN1 NNNCCTCTGTGACTCAGCTG NGG 2 0.2667 Tier II
8 NC_000007.14 7 29425376 - CHN2 NNNCCTCTGTTCCTCAGCTG NGG 2 0.1754 Tier II
9 NC_000015.10 15 64158401 - PPIB NNNCCGCTGTTGCTCTGCTG NGG 2 0.0 Tier II
10 NC_000017.11 17 81861586 + LOC124904086 NNNCAGCTGTTACACAGCTG NGG 2 0.3537 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 55524072 - Dok3 NNNCCGCTGTGACTCAGGTG NGG 2 0.0533 Tier I
2 NC_000071.6 5 24420736 - Cdk5 NNNCCGCTGCTACTCTGCTG NGG 2 0.0 Tier I
3 NC_000070.6 4 154999696 - Plch2 NNNTCGCTGTCACTCAGCTG NGG 2 0.5333 Tier II
4 NC_000076.6 10 118880536 + Dyrk2 NNNCCACTGTGACTCAGCTG NGG 2 0.4 Tier II
5 NC_000081.6 15 75676296 - Top1mt NNNCCGCTTCTACTCAGCTG NGG 2 0.2844 Tier II
6 NC_000075.6 9 82842002 + Irak1bp1 NNNCGGCTGTGACTCAGCTG NGG 2 0.24 Tier II
7 NC_000085.6 19 24465871 - Pip5k1b NNNCCGCTGATCCTCAGCTG NGG 2 0.2256 Tier II
8 NC_000075.6 9 44083994 - Usp2 NNNGCGCTGTTACTCATCTG NGG 2 0.125 Tier II
9 NC_000069.6 3 9431580 - Zfp704 NNNCCGCTGTGACTCAGCCG NGG 2 0.1103 Tier II
10 NC_000080.6 14 57716762 - Lats2 NNNCTGCTGTTACTTAGCTG NGG 2 0.0424 Tier II
11 NC_000085.6 19 3267399 + Ighmbp2 NNNCAGCTGTTACTGAGCTG NGG 2 0.0286 Tier II
12 NC_000075.6 9 65133440 - Igdcc4 NNNCCGCTGTTACTGAGGTG NGG 2 0.0067 Tier II
13 NC_000069.6 3 17601119 - Gm30340 NNNTCCCTGTTACTCAGCTG NGG 2 0.5455 Tier III
14 NC_000076.6 10 118880536 + Gm40777 NNNCCACTGTGACTCAGCTG NGG 2 0.4 Tier III
15 NC_000077.6 11 75271061 + Gm35117 NNNCCGCTGTTCCTCAACTG NGG 2 0.2456 Tier III
16 NC_000075.6 9 44083994 - Gm39325 NNNGCGCTGTTACTCATCTG NGG 2 0.125 Tier III
17 NC_000069.6 3 9431580 - C030034L19Rik NNNCCGCTGTGACTCAGCCG NGG 2 0.1103 Tier III
18 NC_000080.6 14 33920203 - Gm30031 NNNCCACTGTTACTCAGCTG NGT 2 0.0161 Tier III
19 NC_000079.6 13 48126428 - A330033J07Rik NNNCCGCTGTTACTCAGGTG NTG 2 0.0052 Tier III
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Other clones with same target sequence:

(none)