Construct: sgRNA BRDN0001148988
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTCCGCTGTTACTCAGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK5 (1020)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77544
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000007.14 |
7 |
151055537 |
- |
CDK5 |
NNNCCGCTGTTACTCAGCTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000015.10 |
15 |
67098838 |
+ |
SMAD3 |
NNNCCACTGTTGCTCAGCTG |
NGG |
2 |
0.7222 |
Tier II |
3 |
NC_000007.14 |
7 |
133050315 |
- |
CHCHD3 |
NNNCCTCTGTTACTCAGCTT |
NGG |
2 |
0.4667 |
Tier II |
4 |
NC_000004.12 |
4 |
80686595 |
+ |
CFAP299 |
NNNCCGCTGTTAATCAACTG |
NGG |
2 |
0.359 |
Tier II |
5 |
NC_000010.11 |
10 |
106878227 |
+ |
SORCS1 |
NNNCCACTGTTTCTCAGCTG |
NGG |
2 |
0.3333 |
Tier II |
6 |
NC_000001.11 |
1 |
209769536 |
- |
TRAF3IP3 |
NNNCCTGTGTTACTCAGCTG |
NGG |
2 |
0.3137 |
Tier II |
7 |
NC_000006.12 |
6 |
16524519 |
- |
ATXN1 |
NNNCCTCTGTGACTCAGCTG |
NGG |
2 |
0.2667 |
Tier II |
8 |
NC_000007.14 |
7 |
29425376 |
- |
CHN2 |
NNNCCTCTGTTCCTCAGCTG |
NGG |
2 |
0.1754 |
Tier II |
9 |
NC_000015.10 |
15 |
64158401 |
- |
PPIB |
NNNCCGCTGTTGCTCTGCTG |
NGG |
2 |
0.0 |
Tier II |
10 |
NC_000017.11 |
17 |
81861586 |
+ |
LOC124904086 |
NNNCAGCTGTTACACAGCTG |
NGG |
2 |
0.3537 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000079.6 |
13 |
55524072 |
- |
Dok3 |
NNNCCGCTGTGACTCAGGTG |
NGG |
2 |
0.0533 |
Tier I |
2 |
NC_000071.6 |
5 |
24420736 |
- |
Cdk5 |
NNNCCGCTGCTACTCTGCTG |
NGG |
2 |
0.0 |
Tier I |
3 |
NC_000070.6 |
4 |
154999696 |
- |
Plch2 |
NNNTCGCTGTCACTCAGCTG |
NGG |
2 |
0.5333 |
Tier II |
4 |
NC_000076.6 |
10 |
118880536 |
+ |
Dyrk2 |
NNNCCACTGTGACTCAGCTG |
NGG |
2 |
0.4 |
Tier II |
5 |
NC_000081.6 |
15 |
75676296 |
- |
Top1mt |
NNNCCGCTTCTACTCAGCTG |
NGG |
2 |
0.2844 |
Tier II |
6 |
NC_000075.6 |
9 |
82842002 |
+ |
Irak1bp1 |
NNNCGGCTGTGACTCAGCTG |
NGG |
2 |
0.24 |
Tier II |
7 |
NC_000085.6 |
19 |
24465871 |
- |
Pip5k1b |
NNNCCGCTGATCCTCAGCTG |
NGG |
2 |
0.2256 |
Tier II |
8 |
NC_000075.6 |
9 |
44083994 |
- |
Usp2 |
NNNGCGCTGTTACTCATCTG |
NGG |
2 |
0.125 |
Tier II |
9 |
NC_000069.6 |
3 |
9431580 |
- |
Zfp704 |
NNNCCGCTGTGACTCAGCCG |
NGG |
2 |
0.1103 |
Tier II |
10 |
NC_000080.6 |
14 |
57716762 |
- |
Lats2 |
NNNCTGCTGTTACTTAGCTG |
NGG |
2 |
0.0424 |
Tier II |
11 |
NC_000085.6 |
19 |
3267399 |
+ |
Ighmbp2 |
NNNCAGCTGTTACTGAGCTG |
NGG |
2 |
0.0286 |
Tier II |
12 |
NC_000075.6 |
9 |
65133440 |
- |
Igdcc4 |
NNNCCGCTGTTACTGAGGTG |
NGG |
2 |
0.0067 |
Tier II |
13 |
NC_000069.6 |
3 |
17601119 |
- |
Gm30340 |
NNNTCCCTGTTACTCAGCTG |
NGG |
2 |
0.5455 |
Tier III |
14 |
NC_000076.6 |
10 |
118880536 |
+ |
Gm40777 |
NNNCCACTGTGACTCAGCTG |
NGG |
2 |
0.4 |
Tier III |
15 |
NC_000077.6 |
11 |
75271061 |
+ |
Gm35117 |
NNNCCGCTGTTCCTCAACTG |
NGG |
2 |
0.2456 |
Tier III |
16 |
NC_000075.6 |
9 |
44083994 |
- |
Gm39325 |
NNNGCGCTGTTACTCATCTG |
NGG |
2 |
0.125 |
Tier III |
17 |
NC_000069.6 |
3 |
9431580 |
- |
C030034L19Rik |
NNNCCGCTGTGACTCAGCCG |
NGG |
2 |
0.1103 |
Tier III |
18 |
NC_000080.6 |
14 |
33920203 |
- |
Gm30031 |
NNNCCACTGTTACTCAGCTG |
NGT |
2 |
0.0161 |
Tier III |
19 |
NC_000079.6 |
13 |
48126428 |
- |
A330033J07Rik |
NNNCCGCTGTTACTCAGGTG |
NTG |
2 |
0.0052 |
Tier III |
Other clones with same target sequence:
(none)