Construct: sgRNA BRDN0001148989
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCAAGAATGCAGCCCAACA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PRKCI (5584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000003.12 | 3 | 170281231 | - | PRKCI | NNNAAGAATGCAGCCCAACA | NGG | 0 | 1.0 | Tier I |
2 | NC_000023.11 | X | 149541764 | + | EOLA1 | NNNAAGAATGCTGCTCAACA | NGG | 2 | 0.0222 | Tier I |
3 | NC_000004.12 | 4 | 3383048 | - | RGS12 | NNNAAGAAAACAGCCCAACA | NGG | 2 | 0.8667 | Tier II |
4 | NC_000002.12 | 2 | 97714273 | - | ZAP70 | NNNAAGGATGCAGCCAAACA | NGG | 2 | 0.7059 | Tier II |
5 | NC_000012.12 | 12 | 112471578 | - | PTPN11 | NNNAAGAATGAAACCCAACA | NGG | 2 | 0.6923 | Tier II |
6 | NC_000007.14 | 7 | 32399229 | - | PDE1C | NNNAAGAATGCAATCCAACA | NGG | 2 | 0.6769 | Tier II |
7 | NC_000009.12 | 9 | 18031737 | - | ADAMTSL1 | NNNGAGAATGCAGCCCAACA | NGG | 1 | 0.625 | Tier II |
8 | NC_000014.9 | 14 | 92617587 | + | RIN3 | NNNGAGAATACAGCCCAACA | NGG | 2 | 0.5833 | Tier II |
9 | NC_000022.11 | 22 | 32792297 | - | SYN3 | NNNTAGAAGGCAGCCCAACA | NGG | 2 | 0.3939 | Tier II |
10 | NC_000002.12 | 2 | 197192017 | + | ANKRD44 | NNNAAGAATGAAGCCCATCA | NGG | 2 | 0.375 | Tier II |
11 | NC_000010.11 | 10 | 13008199 | + | CCDC3 | NNNAGGCATGCAGCCCAACA | NGG | 2 | 0.315 | Tier II |
12 | NC_000004.12 | 4 | 5874951 | - | CRMP1 | NNNAGGAATGCAGCCCAGCA | NGG | 2 | 0.288 | Tier II |
13 | NC_000002.12 | 2 | 178717402 | + | TTN | NNNAAGAATGAAGACCAACA | NGG | 2 | 0.2625 | Tier II |
14 | NC_000012.12 | 12 | 1283065 | - | ERC1 | NNNAAGAAAGCAGCACAACA | NGG | 2 | 0.2063 | Tier II |
15 | NC_000001.11 | 1 | 75193769 | - | LHX8 | NNNAAGAATTGAGCCCAACA | NGG | 2 | 0.2031 | Tier II |
16 | NC_000008.11 | 8 | 120336850 | - | COL14A1 | NNNAAGAATGCAGCCCAGTA | NGG | 2 | 0.1846 | Tier II |
17 | NC_000023.11 | X | 5965394 | - | NLGN4X | NNNAAGAATGGAGCCCAACT | NGG | 2 | 0.15 | Tier II |
18 | NC_000010.11 | 10 | 20922703 | + | NEBL | NNNCAGAATGCAGCCCAGCA | NGG | 2 | 0.1412 | Tier II |
19 | NC_000001.11 | 1 | 25349062 | + | TMEM50A | NNNAAGAAGGCAGCCCTACA | NGG | 2 | 0.0825 | Tier II |
20 | NC_000021.9 | 21 | 43610146 | + | HSF2BP | NNNAAAAATGCAGCCCAACA | NGA | 2 | 0.0694 | Tier II |
21 | NC_000003.12 | 3 | 17384754 | + | TBC1D5 | NNNAAGAATGCAGCATAACA | NGG | 2 | 0.0684 | Tier II |
22 | NC_000012.12 | 12 | 65056152 | + | WIF1 | NNNAAGAATGCAGCTAAACA | NGG | 2 | 0.0667 | Tier II |
23 | NC_000004.12 | 4 | 882218 | + | GAK | NNNAAGAATGGAGCCCAACA | NAG | 2 | 0.0648 | Tier II |
24 | NC_000005.10 | 5 | 110553449 | - | TMEM232 | NNNAGGAATGCAGCTCAACA | NGG | 2 | 0.048 | Tier II |
25 | NC_000001.11 | 1 | 176908947 | + | ASTN1 | NNNAAGAATGGAGCCCACCA | NGG | 2 | 0.0476 | Tier II |
26 | NC_000004.12 | 4 | 139978184 | + | MAML3 | NNNAAGAATGGAGCCCAAGA | NGG | 2 | 0.0313 | Tier II |
27 | NC_000002.12 | 2 | 136848319 | - | THSD7B | NNNAAGAATGAAGCCCAACA | NTG | 2 | 0.0292 | Tier II |
28 | NC_000012.12 | 12 | 15370244 | - | PTPRO | NNNAAGAATGCAGAGCAACA | NGG | 2 | 0.0175 | Tier II |
29 | NC_000001.11 | 1 | 245290729 | + | KIF26B | NNNAAGAATGCAGCCCAGCA | NGT | 2 | 0.0065 | Tier II |
30 | NC_000004.12 | 4 | 24835336 | + | CCDC149 | NNNAAGAATGCAGCCTAACA | NGT | 2 | 0.005 | Tier II |
31 | NC_000010.11 | 10 | 72470817 | - | MICU1 | NNNAAGAATGCAGCCTAACA | NGT | 2 | 0.005 | Tier II |
32 | NC_000008.11 | 8 | 142487552 | - | ADGRB1 | NNNAAGAATGCAGCCCAAGA | NGC | 2 | 0.0028 | Tier II |
33 | NC_000006.12 | 6 | 56198580 | + | COL21A1 | NNNAAGAATACAGGCCAACA | NGG | 2 | 0.0 | Tier II |
34 | NC_000023.11 | X | 101538325 | + | PRKCIP1 | NNNAAGACTGCAGCCCAACA | NGG | 1 | 0.4286 | Tier III |
35 | NC_000024.10 | Y | 17193695 | - | SURF6P1 | NNNAAGATGGCAGCCCAACA | NGG | 2 | 0.2653 | Tier III |
36 | NC_000020.11 | 20 | 21651304 | - | LINC01726 | NNNGAGAATGCAGCCCAGCA | NGG | 2 | 0.25 | Tier III |
37 | NC_000009.12 | 9 | 31976090 | + | LOC124902137 | NNNAAGAATGAAGCACAACA | NGG | 2 | 0.1667 | Tier III |
38 | NC_000008.11 | 8 | 76656000 | + | ZFHX4-AS1 | NNNAAGAATGCAGCCAAAGA | NGG | 2 | 0.125 | Tier III |
39 | NC_000007.14 | 7 | 20995349 | + | LINC01162 | NNNAAGAATGCAGCCCAAAA | NAG | 2 | 0.1111 | Tier III |
40 | NC_000002.12 | 2 | 127457380 | + | LOC124907884 | NNNATGAATGCAGCCCAACA | NAG | 2 | 0.0943 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 187226162 | + | Gpatch2 | NNNAAGAAGCCAGCCCAACA | NGG | 2 | 0.2476 | Tier I |
2 | NC_000073.6 | 7 | 87493240 | - | Tyr | NNNAAGAATGCTGCCCACCA | NGG | 2 | 0.0635 | Tier I |
3 | NC_000069.6 | 3 | 31038515 | - | Prkci | NNNAAGAATGCAGCCCGACA | NGA | 2 | 0.0123 | Tier I |
4 | NC_000075.6 | 9 | 56753202 | + | Lingo1 | NNNAAGAAAGCAGCCCAACT | NGG | 2 | 0.5571 | Tier II |
5 | NC_000077.6 | 11 | 113356428 | - | Slc39a11 | NNNAAGAATACAGCCCATCA | NGG | 2 | 0.4667 | Tier II |
6 | NC_000086.7 | X | 102804921 | - | Dmrtc1c1 | NNNAAGGATGCAGCCCAACT | NGG | 2 | 0.4235 | Tier II |
7 | NC_000086.7 | X | 102852659 | + | Dmrtc1c2 | NNNAAGGATGCAGCCCAACT | NGG | 2 | 0.4235 | Tier II |
8 | NC_000070.6 | 4 | 88400499 | - | Focad | NNNAAGAATGCAGTCCATCA | NGG | 2 | 0.3667 | Tier II |
9 | NC_000073.6 | 7 | 96952377 | - | Nars2 | NNNATGAATGCAGCCCAACA | NGG | 1 | 0.3636 | Tier II |
10 | NC_000080.6 | 14 | 70719779 | - | Xpo7 | NNNAGGAATGCAGCCCATCA | NGG | 2 | 0.36 | Tier II |
11 | NC_000075.6 | 9 | 116657156 | - | Rbms3 | NNNAAGGATGCAGCCCATCA | NGG | 2 | 0.3529 | Tier II |
12 | NC_000072.6 | 6 | 123790423 | - | Vmn2r24 | NNNAAGAGTGCAGCCCAGCA | NGG | 2 | 0.2933 | Tier II |
13 | NC_000076.6 | 10 | 52834468 | + | Slc35f1 | NNNAAGAATGAAGACCAACA | NGG | 2 | 0.2625 | Tier II |
14 | NC_000081.6 | 15 | 41028428 | + | Zfpm2 | NNNAAGAATGCAGCACAACA | NGG | 1 | 0.2222 | Tier II |
15 | NC_000069.6 | 3 | 138557375 | - | Eif4e | NNNAGGAATGCAGCCCAACA | NAG | 2 | 0.1867 | Tier II |
16 | NC_000076.6 | 10 | 42950823 | + | Scml4 | NNNAGGAATGCAGCCCAACC | NGG | 2 | 0.1636 | Tier II |
17 | NC_000077.6 | 11 | 30445690 | + | 4930505A04Rik | NNNAAGAATGGAGCCCATCA | NGG | 2 | 0.125 | Tier II |
18 | NC_000077.6 | 11 | 26513656 | + | Vrk2 | NNNAGGAATGCAGCCCAAGA | NGG | 2 | 0.09 | Tier II |
19 | NC_000074.6 | 8 | 55030259 | + | Gpm6a | NNNAAGAATGCCTCCCAACA | NGG | 2 | 0.0789 | Tier II |
20 | NC_000076.6 | 10 | 40130113 | - | Slc16a10 | NNNAAGAATGCAGCCCGGCA | NGG | 2 | 0.0706 | Tier II |
21 | NC_000078.6 | 12 | 79616277 | - | Rad51b | NNNAAGAATGCAGCCCATGA | NGG | 2 | 0.0625 | Tier II |
22 | NC_000079.6 | 13 | 73478220 | - | Lpcat1 | NNNCAGAATGCAGCCCAAGA | NGG | 2 | 0.0441 | Tier II |
23 | NC_000067.6 | 1 | 189839773 | - | Ptpn14 | NNNAGGAATGCAGCCCAACA | NTG | 2 | 0.0281 | Tier II |
24 | NC_000067.6 | 1 | 58951428 | + | Trak2 | NNNAAGAATGCAGCCCTCCA | NGG | 2 | 0.0254 | Tier II |
25 | NC_000067.6 | 1 | 150949098 | - | Hmcn1 | NNNAAGAATGCAGCCCCACA | NCG | 2 | 0.0189 | Tier II |
26 | NC_000077.6 | 11 | 108027498 | - | Prkca | NNNAAGAATGCAGCCCAAGA | NGA | 2 | 0.0087 | Tier II |
27 | NC_000077.6 | 11 | 46137763 | - | Adam19 | NNNATGAATGCAGCCCAACA | NGT | 2 | 0.0059 | Tier II |
28 | NC_000071.6 | 5 | 43998357 | + | Prom1 | NNNAAGAATGCAGCCCAACA | NAA | 2 | 0.0 | Tier II |
29 | NC_000072.6 | 6 | 123967061 | + | Gm21023 | NNNGAGAATGAAGCCCAACA | NGG | 2 | 0.4688 | Tier III |
30 | NC_000077.6 | 11 | 113356428 | - | Gm51926 | NNNAAGAATACAGCCCATCA | NGG | 2 | 0.4667 | Tier III |
31 | NC_000085.6 | 19 | 57351794 | + | B230217O12Rik | NNNGAGAATGCAGCCCAAGA | NGG | 2 | 0.0781 | Tier III |
32 | NC_000072.6 | 6 | 143133593 | + | Gm31108 | NNNAAGAATGGAGCCCAACA | NAG | 2 | 0.0648 | Tier III |
33 | NC_000067.6 | 1 | 172270140 | - | LOC115487369 | NNNAAGAATGCATCCCTACA | NGG | 2 | 0.04 | Tier III |