Construct: sgRNA BRDN0001148989

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCAAGAATGCAGCCCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCI (5584)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76401

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 170281231 - PRKCI NNNAAGAATGCAGCCCAACA NGG 0 1.0 Tier I
2 NC_000023.11 X 149541764 + EOLA1 NNNAAGAATGCTGCTCAACA NGG 2 0.0222 Tier I
3 NC_000004.12 4 3383048 - RGS12 NNNAAGAAAACAGCCCAACA NGG 2 0.8667 Tier II
4 NC_000002.12 2 97714273 - ZAP70 NNNAAGGATGCAGCCAAACA NGG 2 0.7059 Tier II
5 NC_000012.12 12 112471578 - PTPN11 NNNAAGAATGAAACCCAACA NGG 2 0.6923 Tier II
6 NC_000007.14 7 32399229 - PDE1C NNNAAGAATGCAATCCAACA NGG 2 0.6769 Tier II
7 NC_000009.12 9 18031737 - ADAMTSL1 NNNGAGAATGCAGCCCAACA NGG 1 0.625 Tier II
8 NC_000014.9 14 92617587 + RIN3 NNNGAGAATACAGCCCAACA NGG 2 0.5833 Tier II
9 NC_000022.11 22 32792297 - SYN3 NNNTAGAAGGCAGCCCAACA NGG 2 0.3939 Tier II
10 NC_000002.12 2 197192017 + ANKRD44 NNNAAGAATGAAGCCCATCA NGG 2 0.375 Tier II
11 NC_000010.11 10 13008199 + CCDC3 NNNAGGCATGCAGCCCAACA NGG 2 0.315 Tier II
12 NC_000004.12 4 5874951 - CRMP1 NNNAGGAATGCAGCCCAGCA NGG 2 0.288 Tier II
13 NC_000002.12 2 178717402 + TTN NNNAAGAATGAAGACCAACA NGG 2 0.2625 Tier II
14 NC_000012.12 12 1283065 - ERC1 NNNAAGAAAGCAGCACAACA NGG 2 0.2063 Tier II
15 NC_000001.11 1 75193769 - LHX8 NNNAAGAATTGAGCCCAACA NGG 2 0.2031 Tier II
16 NC_000008.11 8 120336850 - COL14A1 NNNAAGAATGCAGCCCAGTA NGG 2 0.1846 Tier II
17 NC_000023.11 X 5965394 - NLGN4X NNNAAGAATGGAGCCCAACT NGG 2 0.15 Tier II
18 NC_000010.11 10 20922703 + NEBL NNNCAGAATGCAGCCCAGCA NGG 2 0.1412 Tier II
19 NC_000001.11 1 25349062 + TMEM50A NNNAAGAAGGCAGCCCTACA NGG 2 0.0825 Tier II
20 NC_000021.9 21 43610146 + HSF2BP NNNAAAAATGCAGCCCAACA NGA 2 0.0694 Tier II
21 NC_000003.12 3 17384754 + TBC1D5 NNNAAGAATGCAGCATAACA NGG 2 0.0684 Tier II
22 NC_000012.12 12 65056152 + WIF1 NNNAAGAATGCAGCTAAACA NGG 2 0.0667 Tier II
23 NC_000004.12 4 882218 + GAK NNNAAGAATGGAGCCCAACA NAG 2 0.0648 Tier II
24 NC_000005.10 5 110553449 - TMEM232 NNNAGGAATGCAGCTCAACA NGG 2 0.048 Tier II
25 NC_000001.11 1 176908947 + ASTN1 NNNAAGAATGGAGCCCACCA NGG 2 0.0476 Tier II
26 NC_000004.12 4 139978184 + MAML3 NNNAAGAATGGAGCCCAAGA NGG 2 0.0313 Tier II
27 NC_000002.12 2 136848319 - THSD7B NNNAAGAATGAAGCCCAACA NTG 2 0.0292 Tier II
28 NC_000012.12 12 15370244 - PTPRO NNNAAGAATGCAGAGCAACA NGG 2 0.0175 Tier II
29 NC_000001.11 1 245290729 + KIF26B NNNAAGAATGCAGCCCAGCA NGT 2 0.0065 Tier II
30 NC_000004.12 4 24835336 + CCDC149 NNNAAGAATGCAGCCTAACA NGT 2 0.005 Tier II
31 NC_000010.11 10 72470817 - MICU1 NNNAAGAATGCAGCCTAACA NGT 2 0.005 Tier II
32 NC_000008.11 8 142487552 - ADGRB1 NNNAAGAATGCAGCCCAAGA NGC 2 0.0028 Tier II
33 NC_000006.12 6 56198580 + COL21A1 NNNAAGAATACAGGCCAACA NGG 2 0.0 Tier II
34 NC_000023.11 X 101538325 + PRKCIP1 NNNAAGACTGCAGCCCAACA NGG 1 0.4286 Tier III
35 NC_000024.10 Y 17193695 - SURF6P1 NNNAAGATGGCAGCCCAACA NGG 2 0.2653 Tier III
36 NC_000020.11 20 21651304 - LINC01726 NNNGAGAATGCAGCCCAGCA NGG 2 0.25 Tier III
37 NC_000009.12 9 31976090 + LOC124902137 NNNAAGAATGAAGCACAACA NGG 2 0.1667 Tier III
38 NC_000008.11 8 76656000 + ZFHX4-AS1 NNNAAGAATGCAGCCAAAGA NGG 2 0.125 Tier III
39 NC_000007.14 7 20995349 + LINC01162 NNNAAGAATGCAGCCCAAAA NAG 2 0.1111 Tier III
40 NC_000002.12 2 127457380 + LOC124907884 NNNATGAATGCAGCCCAACA NAG 2 0.0943 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 187226162 + Gpatch2 NNNAAGAAGCCAGCCCAACA NGG 2 0.2476 Tier I
2 NC_000073.6 7 87493240 - Tyr NNNAAGAATGCTGCCCACCA NGG 2 0.0635 Tier I
3 NC_000069.6 3 31038515 - Prkci NNNAAGAATGCAGCCCGACA NGA 2 0.0123 Tier I
4 NC_000075.6 9 56753202 + Lingo1 NNNAAGAAAGCAGCCCAACT NGG 2 0.5571 Tier II
5 NC_000077.6 11 113356428 - Slc39a11 NNNAAGAATACAGCCCATCA NGG 2 0.4667 Tier II
6 NC_000086.7 X 102804921 - Dmrtc1c1 NNNAAGGATGCAGCCCAACT NGG 2 0.4235 Tier II
7 NC_000086.7 X 102852659 + Dmrtc1c2 NNNAAGGATGCAGCCCAACT NGG 2 0.4235 Tier II
8 NC_000070.6 4 88400499 - Focad NNNAAGAATGCAGTCCATCA NGG 2 0.3667 Tier II
9 NC_000073.6 7 96952377 - Nars2 NNNATGAATGCAGCCCAACA NGG 1 0.3636 Tier II
10 NC_000080.6 14 70719779 - Xpo7 NNNAGGAATGCAGCCCATCA NGG 2 0.36 Tier II
11 NC_000075.6 9 116657156 - Rbms3 NNNAAGGATGCAGCCCATCA NGG 2 0.3529 Tier II
12 NC_000072.6 6 123790423 - Vmn2r24 NNNAAGAGTGCAGCCCAGCA NGG 2 0.2933 Tier II
13 NC_000076.6 10 52834468 + Slc35f1 NNNAAGAATGAAGACCAACA NGG 2 0.2625 Tier II
14 NC_000081.6 15 41028428 + Zfpm2 NNNAAGAATGCAGCACAACA NGG 1 0.2222 Tier II
15 NC_000069.6 3 138557375 - Eif4e NNNAGGAATGCAGCCCAACA NAG 2 0.1867 Tier II
16 NC_000076.6 10 42950823 + Scml4 NNNAGGAATGCAGCCCAACC NGG 2 0.1636 Tier II
17 NC_000077.6 11 30445690 + 4930505A04Rik NNNAAGAATGGAGCCCATCA NGG 2 0.125 Tier II
18 NC_000077.6 11 26513656 + Vrk2 NNNAGGAATGCAGCCCAAGA NGG 2 0.09 Tier II
19 NC_000074.6 8 55030259 + Gpm6a NNNAAGAATGCCTCCCAACA NGG 2 0.0789 Tier II
20 NC_000076.6 10 40130113 - Slc16a10 NNNAAGAATGCAGCCCGGCA NGG 2 0.0706 Tier II
21 NC_000078.6 12 79616277 - Rad51b NNNAAGAATGCAGCCCATGA NGG 2 0.0625 Tier II
22 NC_000079.6 13 73478220 - Lpcat1 NNNCAGAATGCAGCCCAAGA NGG 2 0.0441 Tier II
23 NC_000067.6 1 189839773 - Ptpn14 NNNAGGAATGCAGCCCAACA NTG 2 0.0281 Tier II
24 NC_000067.6 1 58951428 + Trak2 NNNAAGAATGCAGCCCTCCA NGG 2 0.0254 Tier II
25 NC_000067.6 1 150949098 - Hmcn1 NNNAAGAATGCAGCCCCACA NCG 2 0.0189 Tier II
26 NC_000077.6 11 108027498 - Prkca NNNAAGAATGCAGCCCAAGA NGA 2 0.0087 Tier II
27 NC_000077.6 11 46137763 - Adam19 NNNATGAATGCAGCCCAACA NGT 2 0.0059 Tier II
28 NC_000071.6 5 43998357 + Prom1 NNNAAGAATGCAGCCCAACA NAA 2 0.0 Tier II
29 NC_000072.6 6 123967061 + Gm21023 NNNGAGAATGAAGCCCAACA NGG 2 0.4688 Tier III
30 NC_000077.6 11 113356428 - Gm51926 NNNAAGAATACAGCCCATCA NGG 2 0.4667 Tier III
31 NC_000085.6 19 57351794 + B230217O12Rik NNNGAGAATGCAGCCCAAGA NGG 2 0.0781 Tier III
32 NC_000072.6 6 143133593 + Gm31108 NNNAAGAATGGAGCCCAACA NAG 2 0.0648 Tier III
33 NC_000067.6 1 172270140 - LOC115487369 NNNAAGAATGCATCCCTACA NGG 2 0.04 Tier III
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Other clones with same target sequence:

(none)