Construct: sgRNA BRDN0001149000

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAAAATGGAGTTCCCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK26 (51765)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75998

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 132068511 - STK26 NNNAAATGGAGTTCCCACAA NGG 0 1.0 Tier I
2 NC_000005.10 5 81335785 + ACOT12 NNNAAATGGGGGTCCCACAA NGG 2 0.2778 Tier I
3 NC_000005.10 5 58726996 + RAB3C NNNAAATGGAGCTCCCAAAA NGG 2 0.5101 Tier II
4 NC_000006.12 6 16143631 + MYLIP NNNAAATGGGGATCCCACAA NGG 2 0.4444 Tier II
5 NC_000001.11 1 201192320 + IGFN1 NNNAAATGGAGTCCCCAAAA NGG 2 0.4251 Tier II
6 NC_000008.11 8 64648464 + CYP7B1 NNNAAATGCAGTTTCCACAA NGG 2 0.3949 Tier II
7 NC_000004.12 4 53039188 - SCFD2 NNNAAATGGGGTTCCCATAA NGG 2 0.3571 Tier II
8 NC_000007.14 7 821829 + SUN1 NNNAAAGGGAGTTCCCACAT NGG 2 0.3529 Tier II
9 NC_000003.12 3 136489163 - STAG1 NNNAAATAGAGTTACCACAA NGG 2 0.35 Tier II
10 NC_000002.12 2 224787786 - DOCK10 NNNTACTGGAGTTCCCACAA NGG 2 0.2893 Tier II
11 NC_000012.12 12 2241007 + CACNA1C NNNAAATGGAGTTACCACAG NGG 2 0.2676 Tier II
12 NC_000003.12 3 61687795 - PTPRG NNNAAATGGATTTCCCAAAA NGG 2 0.2071 Tier II
13 NC_000009.12 9 104789742 + ABCA1 NNNAAATGTATTTCCCACAA NGG 2 0.2051 Tier II
14 NC_000009.12 9 104789742 + NIPSNAP3B NNNAAATGTATTTCCCACAA NGG 2 0.2051 Tier II
15 NC_000020.11 20 46270652 + CDH22 NNNAAATGGAGTTCCCATAC NGG 2 0.1461 Tier II
16 NC_000019.10 19 54617261 - LILRB1 NNNCAATGGAGTGCCCACAA NGG 2 0.0921 Tier II
17 NC_000010.11 10 87820549 + ATAD1 NNNAAATGGAGTTCCCAGTA NGG 2 0.0718 Tier II
18 NC_000004.12 4 108665320 - OSTC NNNAAATGGAGTTACCTCAA NGG 2 0.0467 Tier II
19 NC_000009.12 9 114064990 + AMBP NNNAAATGGAGTTCACAGAA NGG 2 0.0296 Tier II
20 NC_000008.11 8 98203706 + NIPAL2 NNNAAATGCAGTTCCCACAA NTG 2 0.021 Tier II
21 NC_000016.10 16 72903666 - ZFHX3 NNNAAATGGAGTTGTCACAA NGG 2 0.0 Tier II
22 NC_000012.12 12 79759502 - PPP1R12A-AS2 NNNAAATGGAATTTCCACAA NGG 2 0.7333 Tier III
23 NC_000009.12 9 40961103 + LOC107986997 NNNGGATGGAGTTCCCACAA NGG 2 0.45 Tier III
24 NC_000023.11 X 49149027 - LOC105373195 NNNTAAGGGAGTTCCCACAA NGG 2 0.3743 Tier III
25 NC_000004.12 4 53039188 - LOC124900701 NNNAAATGGGGTTCCCATAA NGG 2 0.3571 Tier III
26 NC_000005.10 5 128695476 - LOC105379168 NNNAAATGGAGTTACCACAA NGG 1 0.35 Tier III
27 NC_000003.12 3 61687795 - LOC124906245 NNNAAATGGATTTCCCAAAA NGG 2 0.2071 Tier III
28 NC_000002.12 2 230994172 + SPATA3-AS1 NNNAAATGGAGTCCCCTCAA NGG 2 0.1053 Tier III
29 NC_000003.12 3 153234170 - LOC105374164 NNNAAATGGAGTTTCCAGAA NGG 2 0.0978 Tier III
30 NC_000010.11 10 87820549 + CFL1P1 NNNAAATGGAGTTCCCAGTA NGG 2 0.0718 Tier III
31 NC_000008.11 8 98203706 + NIPAL2-AS1 NNNAAATGCAGTTCCCACAA NTG 2 0.021 Tier III
32 NC_000011.10 11 4666712 - LOC105376528 NNNAAATGGAGCTCCCACAA NGT 2 0.0153 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 97904695 - Pak1 NNNATATGGAGTTCCCACCA NGG 2 0.0752 Tier I
2 NC_000067.6 1 124306668 + Dpp10 NNNGAATGGTGTTCCCACAA NGG 2 0.5515 Tier II
3 NC_000074.6 8 121745824 - Jph3 NNNAAATGGTGTTCCCACCA NGG 2 0.1826 Tier II
4 NC_000070.6 4 103743279 - Dab1 NNNAAATGAAGTGCCCACAA NGG 2 0.1677 Tier II
5 NC_000083.6 17 84377500 + Thada NNNAAATGTAGTTCCCTCAA NGG 2 0.0711 Tier II
6 NC_000083.6 17 17407367 - Lix1 NNNAAATGGAGTTCCTTCAA NGG 2 0.041 Tier II
7 NC_000069.6 3 20115988 - Hltf NNNAAATGAAGTTCCCACAA NTG 2 0.025 Tier II
8 NC_000079.6 13 113041834 - Cdc20b NNNAAATGGCGTTCTCACAA NGG 2 0.0222 Tier II
9 NC_000072.6 6 72305206 + Sftpb NNNAATTGGAGTTCCCACAA NGC 2 0.0159 Tier II
10 NC_000083.6 17 75544936 + Fam98a NNNAAATGGAGTTCCCACTA NGC 2 0.012 Tier II
11 NC_000072.6 6 146471042 + Itpr2 NNNCAATGGAGTTCCCACAA NGC 2 0.0078 Tier II
12 NC_000079.6 13 88843448 + Edil3 NNNAAATGGAGTTCCCAGAA NGC 2 0.003 Tier II
13 NC_000067.6 1 168676145 - Gm34320 NNNAAAGGGAGTACCCACAA NGG 2 0.4072 Tier III
14 NC_000080.6 14 84424418 + Gm32498 NNNATATGGAGTTCCAACAA NGG 2 0.3636 Tier III
15 NC_000068.7 2 106411766 - Gm36036 NNNAAAGGGAGTTCCCACAA NTG 2 0.0229 Tier III
16 NC_000068.7 2 106411766 - Gm46755 NNNAAAGGGAGTTCCCACAA NTG 2 0.0229 Tier III
17 NC_000075.6 9 89838952 + 4930524O08Rik NNNACATGGAGTTCCCACAA NTG 2 0.0195 Tier III
18 NC_000069.6 3 121564355 + Gm33547 NNNAAATGGAGTGGCCACAA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)