Construct: sgRNA BRDN0001149001
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCATCCAAAGATTGCAAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK3 (8444)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75548
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000071.6 |
5 |
44785022 |
+ |
Ldb2 |
NNNAACCAAAGACTGCAAGA |
NGG |
2 |
0.3947 |
Tier II |
2 |
NC_000075.6 |
9 |
94793218 |
+ |
Slc9a9 |
NNNATGCGAAGATTGCAAGA |
NGG |
2 |
0.3667 |
Tier II |
3 |
NC_000067.6 |
1 |
19133331 |
+ |
Tfap2d |
NNNATCCAAATATTACAAGA |
NGG |
2 |
0.362 |
Tier II |
4 |
NC_000072.6 |
6 |
94100941 |
- |
Magi1 |
NNNCTCCAAAGATTGAAAGA |
NGG |
2 |
0.3529 |
Tier II |
5 |
NC_000070.6 |
4 |
104411431 |
- |
Dab1 |
NNNATCCAAAGATAGGAAGA |
NGG |
2 |
0.0952 |
Tier II |
6 |
NC_000082.6 |
16 |
29328471 |
+ |
Atp13a5 |
NNNAACCAAAGATTGGAAGA |
NGG |
2 |
0.0769 |
Tier II |
7 |
NC_000069.6 |
3 |
24623191 |
+ |
Naaladl2 |
NNNCTCCAAAGATTGCCAGA |
NGG |
2 |
0.0623 |
Tier II |
8 |
NC_000083.6 |
17 |
7180012 |
- |
Rps6ka2 |
NNNATCCAGAGATTGCAAGA |
NGA |
2 |
0.0463 |
Tier II |
9 |
NC_000084.6 |
18 |
72098416 |
- |
Dcc |
NNNATCCAAAGATTGCAATA |
NGT |
2 |
0.0108 |
Tier II |
10 |
NC_000071.6 |
5 |
91578560 |
- |
Parm1 |
NNNATCCAAAGTTTGCAAGA |
NGC |
2 |
0.0074 |
Tier II |
11 |
NC_000086.7 |
X |
103440317 |
- |
Tsix |
NNNATCCAAAGAATGAAAGA |
NGG |
2 |
0.6923 |
Tier III |
12 |
NC_000084.6 |
18 |
45820572 |
+ |
A330093E20Rik |
NNNATCCAGAGATTACAAGA |
NGG |
2 |
0.6275 |
Tier III |
13 |
NC_000069.6 |
3 |
58695878 |
- |
4930593A02Rik |
NNNATCCAGAGATTGCAAAA |
NGG |
2 |
0.4762 |
Tier III |
14 |
NC_000076.6 |
10 |
39609080 |
- |
Gm16364 |
NNNATCCAAAGGTAGCAAGA |
NGG |
2 |
0.4471 |
Tier III |
15 |
NC_000075.6 |
9 |
90697592 |
- |
Gm30905 |
NNNATCCAGAGATTGCAGGA |
NGG |
2 |
0.2667 |
Tier III |
16 |
NC_000070.6 |
4 |
13003077 |
- |
Gm42256 |
NNNCTCCAGAGATTGCAAGA |
NGG |
2 |
0.2353 |
Tier III |
17 |
NC_000086.7 |
X |
25078694 |
- |
Gm42218 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
18 |
NC_000086.7 |
X |
27389558 |
+ |
Gm42101 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
19 |
NC_000086.7 |
X |
27687074 |
- |
Gm2101 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
20 |
NC_000086.7 |
X |
27841326 |
+ |
Gm42173 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
21 |
NC_000086.7 |
X |
28188999 |
+ |
Gm42175 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
22 |
NC_000086.7 |
X |
28502303 |
+ |
Gm42177 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
23 |
NC_000086.7 |
X |
28849107 |
+ |
Gm42179 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
24 |
NC_000086.7 |
X |
29189250 |
+ |
Gm42181 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
25 |
NC_000086.7 |
X |
29732460 |
- |
Gm42098 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
26 |
NC_000086.7 |
X |
30081840 |
- |
E330016L19Rik |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
27 |
NC_000086.7 |
X |
30417592 |
- |
Gm7437 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
28 |
NC_000086.7 |
X |
30743229 |
+ |
Gm42182 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
29 |
NC_000086.7 |
X |
34832161 |
- |
Gm42403 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
30 |
NC_000086.7 |
X |
35172984 |
- |
Gm2309 |
NNNATCCAGAGATTGCCAGA |
NGG |
2 |
0.1176 |
Tier III |
31 |
NC_000072.6 |
6 |
68851769 |
+ |
Igk |
NNNATCCAAAGAATTCAAGA |
NGG |
2 |
0.0989 |
Tier III |
32 |
NC_000072.6 |
6 |
68111519 |
+ |
Igk |
NNNCTCCAAAGATTGCCAGA |
NGG |
2 |
0.0623 |
Tier III |
33 |
NC_000069.6 |
3 |
52707033 |
- |
Gm30235 |
NNNATCCAAAGATTGCAAAA |
NTG |
2 |
0.0278 |
Tier III |
34 |
NC_000072.6 |
6 |
6856779 |
- |
Dlx6os1 |
NNNATCCAAAAATTGCAAGA |
NGC |
2 |
0.0222 |
Tier III |
35 |
NC_000073.6 |
7 |
14713219 |
- |
Obox4-ps4 |
NNNATCCAAAGAATGCAAGA |
NGT |
2 |
0.0112 |
Tier III |
Other clones with same target sequence:
(none)