Construct: sgRNA BRDN0001149002
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTTTGTATACGCTGCCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK4 (6789)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76074
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
44978451 |
- |
STK4 |
NNNTTTGTATACGCTGCCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
21842787 |
- |
ABCC9 |
NNNTTTGTATGCACTGCCAT |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000015.10 |
15 |
31800855 |
- |
OTUD7A |
NNNTTTGTAGACGCTGCCAT |
NGC |
2 |
0.0111 |
Tier II |
| 4 |
NC_000006.12 |
6 |
30018254 |
+ |
POLR1HASP |
NNNTGTGTATACACTGCCAT |
NGG |
2 |
0.5908 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
97062432 |
+ |
Osbpl7 |
NNNTGTGTGTACGCTGCCAT |
NGG |
2 |
0.4267 |
Tier II |
| 2 |
NC_000084.6 |
18 |
63069668 |
- |
Piezo2 |
NNNTTTGGATACGCTGCCTT |
NGG |
2 |
0.3949 |
Tier II |
| 3 |
NC_000068.7 |
2 |
54604794 |
- |
Galnt13 |
NNNTTTGTGTATGCTGCCAT |
NGG |
2 |
0.359 |
Tier II |
| 4 |
NC_000078.6 |
12 |
79216393 |
- |
Rdh12 |
NNNTTGGTATAGGCTGCCAT |
NGG |
2 |
0.254 |
Tier II |
| 5 |
NC_000075.6 |
9 |
77464011 |
- |
Lrrc1 |
NNNTTTGTATATGCTGCCCT |
NGG |
2 |
0.1114 |
Tier II |
| 6 |
NC_000068.7 |
2 |
170611790 |
+ |
Gm52537 |
NNNTTTATAGACGCTGCCAT |
NGG |
2 |
0.5 |
Tier III |
| 7 |
NC_000078.6 |
12 |
23100921 |
- |
Gm9293 |
NNNTTTCTATAGGCTGCCAT |
NGG |
2 |
0.3056 |
Tier III |
| 8 |
NC_000078.6 |
12 |
23721798 |
- |
Gm9305 |
NNNTTTCTATAGGCTGCCAT |
NGG |
2 |
0.3056 |
Tier III |
Other clones with same target sequence:
(none)
