Construct: sgRNA BRDN0001149003
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTTGCAAGAATTATGCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CASK (8573)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77404
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
41569692 |
- |
CASK |
NNNTGCAAGAATTATGCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
98918009 |
- |
PLPPR5 |
NNNTACAAGAATTATACATG |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000016.10 |
16 |
55824346 |
- |
CES1 |
NNNTGCAAGAATCATGCATG |
NGG |
1 |
0.7895 |
Tier II |
| 4 |
NC_000001.11 |
1 |
167648055 |
- |
RCSD1 |
NNNTGTAAGAATTATGCATT |
NGG |
2 |
0.65 |
Tier II |
| 5 |
NC_000005.10 |
5 |
20004412 |
- |
CDH18 |
NNNTGCAAGAATTATGTATA |
NGG |
2 |
0.4375 |
Tier II |
| 6 |
NC_000015.10 |
15 |
89053644 |
+ |
ABHD2 |
NNNTGGAAGAATCATGCATG |
NGG |
2 |
0.3947 |
Tier II |
| 7 |
NC_000010.11 |
10 |
23952996 |
+ |
KIAA1217 |
NNNTGCAGGAATTTTGCATG |
NGG |
2 |
0.3911 |
Tier II |
| 8 |
NC_000014.9 |
14 |
92953195 |
+ |
ITPK1 |
NNNCGCAACAATTATGCATG |
NGG |
2 |
0.3484 |
Tier II |
| 9 |
NC_000001.11 |
1 |
57974706 |
- |
DAB1 |
NNNTGCAAGACATATGCATG |
NGG |
2 |
0.32 |
Tier II |
| 10 |
NC_000020.11 |
20 |
42930786 |
- |
PTPRT |
NNNCGCAAGAATTATGTATG |
NGG |
2 |
0.302 |
Tier II |
| 11 |
NC_000004.12 |
4 |
24393711 |
- |
PPARGC1A |
NNNGGCAAGAATTGTGCATG |
NGG |
2 |
0.2917 |
Tier II |
| 12 |
NC_000002.12 |
2 |
137466087 |
+ |
THSD7B |
NNNTGCAAGTATTATGCAGG |
NGG |
2 |
0.2206 |
Tier II |
| 13 |
NC_000008.11 |
8 |
117068477 |
- |
SLC30A8 |
NNNTGCAAAAATGATGCATG |
NGG |
2 |
0.1677 |
Tier II |
| 14 |
NC_000012.12 |
12 |
40827881 |
+ |
CNTN1 |
NNNTGCATGAATTATGCATG |
NAG |
2 |
0.1111 |
Tier II |
| 15 |
NC_000008.11 |
8 |
71063450 |
+ |
XKR9 |
NNNAGCAAGAATTCTGCATG |
NGG |
2 |
0.102 |
Tier II |
| 16 |
NC_000016.10 |
16 |
57624896 |
+ |
ADGRG1 |
NNNTGCAAGATTTACGCATG |
NGG |
2 |
0.0839 |
Tier II |
| 17 |
NC_000018.10 |
18 |
59338461 |
- |
LMAN1 |
NNNTGCAAGATTTCTGCATG |
NGG |
2 |
0.0659 |
Tier II |
| 18 |
NC_000001.11 |
1 |
160612959 |
- |
SLAMF1 |
NNNTGCAAGAATTCTGCATG |
NAG |
2 |
0.0556 |
Tier II |
| 19 |
NC_000015.10 |
15 |
52488148 |
+ |
MYO5A |
NNNTGAAAGAATTATGGATG |
NGG |
2 |
0.0546 |
Tier II |
| 20 |
NC_000002.12 |
2 |
140781322 |
- |
LRP1B |
NNNAGCAAGAATTATGCATG |
NTG |
2 |
0.0186 |
Tier II |
| 21 |
NC_000005.10 |
5 |
169092641 |
- |
SLIT3 |
NNNTGCAAGAATTATGCGTG |
NTG |
2 |
0.0156 |
Tier II |
| 22 |
NC_000020.11 |
20 |
38496917 |
- |
RALGAPB |
NNNTGCAAGAATTCTGCATG |
NGA |
2 |
0.0149 |
Tier II |
| 23 |
NC_000012.12 |
12 |
62758242 |
- |
PPM1H |
NNNTCCAAGAATTATTCATG |
NGG |
2 |
0.0 |
Tier II |
| 24 |
NC_000001.11 |
1 |
225704823 |
- |
LOC102723834 |
NNNTGTAAGAATTATGCATG |
NGG |
1 |
0.9286 |
Tier III |
| 25 |
NC_000016.10 |
16 |
55769300 |
+ |
CES1P1 |
NNNTGCAAGAATCATGCATG |
NGG |
1 |
0.7895 |
Tier III |
| 26 |
NC_000015.10 |
15 |
89053644 |
+ |
CARMAL |
NNNTGGAAGAATCATGCATG |
NGG |
2 |
0.3947 |
Tier III |
| 27 |
NC_000001.11 |
1 |
57974706 |
- |
LOC105378746 |
NNNTGCAAGACATATGCATG |
NGG |
2 |
0.32 |
Tier III |
| 28 |
NC_000009.12 |
9 |
2454086 |
+ |
LOC101930053 |
NNNTGCAAGAATTATGGATG |
NGA |
2 |
0.0041 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
13559344 |
- |
Cask |
NNNCGCAAGAATCATGCATG |
NGG |
2 |
0.5108 |
Tier I |
| 2 |
NC_000072.6 |
6 |
33683049 |
- |
Exoc4 |
NNNTGAAAGAAATATGCATG |
NGG |
2 |
0.7429 |
Tier II |
| 3 |
NC_000073.6 |
7 |
144133035 |
+ |
Shank2 |
NNNTGCAAGTATTAAGCATG |
NGG |
2 |
0.5108 |
Tier II |
| 4 |
NC_000084.6 |
18 |
39196421 |
+ |
Arhgap26 |
NNNTGAAAGAAGTATGCATG |
NGG |
2 |
0.4643 |
Tier II |
| 5 |
NC_000084.6 |
18 |
35835753 |
+ |
Cxxc5 |
NNNTGCAAGGATTAAGCATG |
NGG |
2 |
0.3216 |
Tier II |
| 6 |
NC_000081.6 |
15 |
80601612 |
+ |
Grap2 |
NNNTGCATAAATTATGCATG |
NGG |
2 |
0.2755 |
Tier II |
| 7 |
NC_000070.6 |
4 |
57779496 |
- |
Pakap |
NNNTGCAAGAAGTATGCTTG |
NGG |
2 |
0.25 |
Tier II |
| 8 |
NC_000080.6 |
14 |
79590223 |
- |
Sugt1 |
NNNTGCAAGAATCATGCAGG |
NGG |
2 |
0.1974 |
Tier II |
| 9 |
NC_000072.6 |
6 |
126140937 |
+ |
Ntf3 |
NNNTGCAAAAATTATGCAAG |
NGG |
2 |
0.1837 |
Tier II |
| 10 |
NC_000069.6 |
3 |
28411014 |
- |
Tnik |
NNNGGCAAGAATTCTGCATG |
NGG |
2 |
0.1339 |
Tier II |
| 11 |
NC_000079.6 |
13 |
103253874 |
- |
Mast4 |
NNNTGCAAGCATGATGCATG |
NGG |
2 |
0.087 |
Tier II |
| 12 |
NC_000077.6 |
11 |
66223410 |
- |
Shisa6 |
NNNTGCAAGAATTCTGCAAG |
NGG |
2 |
0.0612 |
Tier II |
| 13 |
NC_000076.6 |
10 |
10831381 |
- |
Grm1 |
NNNTGCAAGAATTTTGCATG |
NGA |
2 |
0.037 |
Tier II |
| 14 |
NC_000070.6 |
4 |
100788818 |
+ |
Cachd1 |
NNNTGCAAGAATTGTCCATG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000073.6 |
7 |
86126728 |
+ |
Vmn2r-ps84 |
NNNTGCAAGAATTAAGAATG |
NGG |
2 |
0.2702 |
Tier III |
| 16 |
NC_000074.6 |
8 |
18969681 |
+ |
Gm40464 |
NNNTACAAGAATTATGCAAG |
NGG |
2 |
0.2476 |
Tier III |
| 17 |
NC_000083.6 |
17 |
24467288 |
+ |
4833447I15Rik |
NNNTTCAAGAATAATGCATG |
NGG |
2 |
0.2077 |
Tier III |
| 18 |
NC_000075.6 |
9 |
23053640 |
+ |
Gm36743 |
NNNTGCATGATTTATGCATG |
NGG |
2 |
0.1319 |
Tier III |
| 19 |
NC_000077.6 |
11 |
66223410 |
- |
Gm12296 |
NNNTGCAAGAATTCTGCAAG |
NGG |
2 |
0.0612 |
Tier III |
| 20 |
NC_000082.6 |
16 |
42837571 |
- |
4932412D23Rik |
NNNTCCAAGAATTATGGATG |
NGG |
2 |
0.0462 |
Tier III |
Other clones with same target sequence:
(none)
