Construct: sgRNA BRDN0001149006
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGATTATGTAGCTACGAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL4 (344387)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76107
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
39190468 |
- |
CDKL4 |
NNNATTATGTAGCTACGAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
10808815 |
- |
MAK |
NNNATTATGTATCTACCAGA |
NGG |
2 |
0.0905 |
Tier I |
| 3 |
NC_000006.12 |
6 |
53019232 |
- |
CILK1 |
NNNATTATGTATCTACCAGA |
NGG |
2 |
0.0905 |
Tier I |
| 4 |
NC_000006.12 |
6 |
96909551 |
- |
KLHL32 |
NNNATTATGGAGCTAAGAGA |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000008.11 |
8 |
117083701 |
+ |
SLC30A8 |
NNNATCATGTAGCTATGAGA |
NGG |
2 |
0.2797 |
Tier II |
| 6 |
NC_000004.12 |
4 |
5926258 |
+ |
C4orf50 |
NNNATTATTTAGCCACGAGA |
NGG |
2 |
0.1524 |
Tier II |
| 7 |
NC_000002.12 |
2 |
29496613 |
- |
ALK |
NNNATTATGAAGCTAGGAGA |
NGG |
2 |
0.1319 |
Tier II |
| 8 |
NC_000002.12 |
2 |
134500983 |
+ |
TMEM163 |
NNNATTATATAGCTAGGAGA |
NGG |
2 |
0.0989 |
Tier II |
| 9 |
NC_000014.9 |
14 |
79421469 |
+ |
NRXN3 |
NNNATTATGTAGATACCAGA |
NGG |
2 |
0.0905 |
Tier II |
| 10 |
NC_000006.12 |
6 |
18445732 |
- |
RNF144B |
NNNATTATGTAGCTTTGAGA |
NGG |
2 |
0.0615 |
Tier II |
| 11 |
NC_000008.11 |
8 |
78462133 |
- |
LOC105375911 |
NNNATTATGTAGGTACCAGA |
NGG |
2 |
0.0321 |
Tier III |
| 12 |
NC_000009.12 |
9 |
104458622 |
- |
LOC105376195 |
NNNATTATGTAGCTAAGAGA |
NGT |
2 |
0.0161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
162376985 |
- |
Ptprt |
NNNATTATGTATCTACGAAA |
NGG |
2 |
0.2747 |
Tier II |
| 2 |
NC_000069.6 |
3 |
108612914 |
- |
Wdr47 |
NNNATTATGTAGCTAAGAGA |
NAG |
2 |
0.2593 |
Tier II |
| 3 |
NC_000069.6 |
3 |
27895611 |
- |
Tmem212 |
NNNATTATGTAGCTATGAGC |
NGG |
2 |
0.0699 |
Tier II |
| 4 |
NC_000077.6 |
11 |
109570658 |
+ |
Arsg |
NNNATTATGTAGCTCCGAGC |
NGG |
2 |
0.062 |
Tier II |
| 5 |
NC_000071.6 |
5 |
116129329 |
- |
Ccdc60 |
NNNATTATGTAGGTAGGAGA |
NGG |
2 |
0.021 |
Tier II |
Other clones with same target sequence:
(none)
