Construct: sgRNA BRDN0001149014
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAGGGCTTTAGATCTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK3 (5595)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75966
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
30118398 |
+ |
MAPK3 |
NNNAGGGCTTTAGATCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
89955283 |
+ |
DEF8 |
NNNAAGGTTTTAGATCTCGG |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000001.11 |
1 |
245374234 |
- |
KIF26B |
NNNAGGGCTTCTGATCTCGG |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000001.11 |
1 |
92673365 |
+ |
EVI5 |
NNNAGGGCTTTAGATCTCTG |
NGT |
2 |
0.0108 |
Tier II |
| 5 |
NC_000009.12 |
9 |
16802499 |
- |
BNC2 |
NNNAGGGCTTTAGATCTCTG |
NGT |
2 |
0.0108 |
Tier II |
| 6 |
NC_000003.12 |
3 |
50943837 |
+ |
DOCK3 |
NNNAGGGCTTTAGATCTGGG |
NTG |
2 |
0.0052 |
Tier II |
| 7 |
NC_000013.11 |
13 |
50502385 |
- |
DLEU1 |
NNNAGGGCTTTAGATCCCTG |
NGG |
2 |
0.4706 |
Tier III |
| 8 |
NC_000014.9 |
14 |
97776952 |
- |
LOC105370651 |
NNNAGGGCTTTAGATGTCAG |
NGG |
2 |
0.1099 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
21293190 |
- |
Cpsf3 |
NNNAGGGCTTTAGATATCAG |
NGG |
2 |
0.7143 |
Tier II |
| 2 |
NC_000068.7 |
2 |
140978385 |
- |
Macrod2 |
NNNAGGGCTTTAAATCTCAG |
NGG |
2 |
0.6593 |
Tier II |
| 3 |
NC_000071.6 |
5 |
147862940 |
- |
Pomp |
NNNAGGGCTATAGATCTCAG |
NGG |
2 |
0.6122 |
Tier II |
| 4 |
NC_000067.6 |
1 |
84835313 |
- |
Trip12 |
NNNAGGGCTTTAGATCTCTT |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000082.6 |
16 |
27398449 |
- |
Ccdc50 |
NNNAGGGCTTTAGATCTCCT |
NGG |
2 |
0.3138 |
Tier II |
| 6 |
NC_000085.6 |
19 |
10800842 |
+ |
Cd6 |
NNNAGGGCTTTAGATTTCTG |
NGG |
2 |
0.2051 |
Tier II |
| 7 |
NC_000077.6 |
11 |
84219565 |
+ |
Acaca |
NNNAGGGCTTTAAATCTCGG |
NGA |
2 |
0.0641 |
Tier II |
| 8 |
NC_000071.6 |
5 |
66604217 |
- |
Apbb2 |
NNNAGGGCTTTAGATTTGGG |
NGG |
2 |
0.041 |
Tier II |
| 9 |
NC_000075.6 |
9 |
105221713 |
- |
Nek11 |
NNNAGGGCTTTAGAGCTGGG |
NGG |
2 |
0.0067 |
Tier II |
| 10 |
NC_000073.6 |
7 |
112691042 |
+ |
Tead1 |
NNNAGGGCTTTAGATCTGGG |
NTG |
2 |
0.0052 |
Tier II |
| 11 |
NC_000068.7 |
2 |
156857576 |
- |
5430405H02Rik |
NNNAGGGCTTTAGCTCTCGG |
NAG |
2 |
0.0556 |
Tier III |
Other clones with same target sequence:
(none)
