Construct: sgRNA BRDN0001149020
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGACGGTGTCATCCAGTGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRM (6723)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76280
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
11059302 |
- |
SRM |
NNNCGGTGTCATCCAGTGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
115073807 |
+ |
TNC |
NNNCGGTGTCTTCCAGAGCA |
NGG |
2 |
0.1641 |
Tier I |
| 3 |
NC_000012.12 |
12 |
123056674 |
- |
PITPNM2 |
NNNCAGTGTCACCCAGTGCA |
NGG |
2 |
0.8211 |
Tier II |
| 4 |
NC_000002.12 |
2 |
88093453 |
- |
SMYD1 |
NNNAGGTGTGATCCAGTGCA |
NGG |
2 |
0.3275 |
Tier II |
| 5 |
NC_000009.12 |
9 |
14791443 |
+ |
FREM1 |
NNNCGGTGTCATGCAGTTCA |
NGG |
2 |
0.0909 |
Tier II |
| 6 |
NC_000017.11 |
17 |
77410321 |
+ |
SEPTIN9 |
NNNCGGTCTCATCCAGTGCA |
NGT |
2 |
0.0099 |
Tier II |
| 7 |
NC_000023.11 |
X |
148652524 |
+ |
AFF2 |
NNNCAGTGTCATCCATTGCA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000002.12 |
2 |
20107881 |
+ |
LAPTM4A-DT |
NNNTGGGGTCATCCAGTGCA |
NGG |
2 |
0.4706 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
128717767 |
- |
Pmel |
NNNCGGTGTCATCCAGTAGA |
NGG |
2 |
0.0865 |
Tier I |
| 2 |
NC_000072.6 |
6 |
99217750 |
- |
Foxp1 |
NNNCGGTGGGATCCAGTGCA |
NGG |
2 |
0.2407 |
Tier II |
| 3 |
NC_000068.7 |
2 |
167156364 |
- |
Kcnb1 |
NNNCGGTGTCATCCAGAGAA |
NGG |
2 |
0.2286 |
Tier II |
| 4 |
NC_000078.6 |
12 |
21203696 |
+ |
Asap2 |
NNNCGGTGTGATCCAGAGCA |
NGG |
2 |
0.2074 |
Tier II |
| 5 |
NC_000067.6 |
1 |
178868052 |
- |
Kif26b |
NNNCTGTCTCATCCAGTGCA |
NGG |
2 |
0.1846 |
Tier II |
| 6 |
NC_000082.6 |
16 |
37533899 |
- |
Gtf2e1 |
NNNCGGTGTCATCCAGAGCA |
NCG |
2 |
0.0571 |
Tier II |
| 7 |
NC_000068.7 |
2 |
93822154 |
- |
Ext2 |
NNNCGGTGTCGTCCAGTGCA |
NGT |
2 |
0.0105 |
Tier II |
| 8 |
NC_000072.6 |
6 |
99217750 |
- |
Gm52870 |
NNNCGGTGGGATCCAGTGCA |
NGG |
2 |
0.2407 |
Tier III |
| 9 |
NC_000072.6 |
6 |
135531581 |
+ |
Gm25136 |
NNNTGGTGTCATCCAGTGCA |
NTG |
2 |
0.0312 |
Tier III |
| 10 |
NC_000084.6 |
18 |
64170346 |
- |
St8sia3os |
NNNGGGTGTCATCCAGTGCA |
NTG |
2 |
0.0195 |
Tier III |
Other clones with same target sequence:
(none)
