Construct: sgRNA BRDN0001149022

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AACCTGAATAATATATAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TWF1 (5756)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77287

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 43802367 + TWF1 NNNCTGAATAATATATAGCA NGG 0 1.0 Tier I
2 NC_000012.12 12 43802367 + TMEM117 NNNCTGAATAATATATAGCA NGG 0 1.0 Tier II
3 NC_000005.10 5 60280783 + PDE4D NNNATAAATAATATATAGCA NGG 2 0.8421 Tier II
4 NC_000014.9 14 61400763 - PRKCH NNNTGGAATAATATATAGCA NGG 2 0.512 Tier II
5 NC_000009.12 9 86014818 - NAA35 NNNTTGAAGAATATATAGCA NGG 2 0.4952 Tier II
6 NC_000005.10 5 34670990 + RAI14 NNNCTGAATAGTATATAACA NGG 2 0.45 Tier II
7 NC_000001.11 1 217431923 - GPATCH2 NNNCTGAATAATAAATAACA NGG 2 0.4286 Tier II
8 NC_000023.11 X 155291176 + CLIC2 NNNCTGAATAATATATAGCA NAG 1 0.2593 Tier II
9 NC_000005.10 5 131432027 + RAPGEF6 NNNATGAATAATTTATAGCA NGG 2 0.2526 Tier II
10 NC_000014.9 14 33173441 + NPAS3 NNNCTGAATTATATATAGCA NAG 2 0.2288 Tier II
11 NC_000015.10 15 34985972 + ZNF770 NNNCTGCATACTATATAGCA NGG 2 0.175 Tier II
12 NC_000001.11 1 55174478 + USP24 NNNCTGAATAATATAAGGCA NGG 2 0.1604 Tier II
13 NC_000011.10 11 75658029 - MAP6 NNNCAGAATATTATATAGCA NGG 2 0.1538 Tier II
14 NC_000004.12 4 182458195 + TENM3 NNNCTGATTAATATCTAGCA NGG 2 0.1169 Tier II
15 NC_000012.12 12 110143259 - IFT81 NNNCTGTATAATATATAGCA NAG 2 0.1134 Tier II
16 NC_000001.11 1 155922705 - KHDC4 NNNCTTAATAATATATAGGA NGG 2 0.0833 Tier II
17 NC_000011.10 11 19941456 + NAV2 NNNCTGAATAATAAATAGCA NGA 2 0.043 Tier II
18 NC_000002.12 2 119712990 - TMEM177 NNNCTAAATAATATATAGCA NTG 2 0.039 Tier II
19 NC_000018.10 18 6076279 + L3MBTL4 NNNCTGAATAATATGTAGCA NTG 2 0.0253 Tier II
20 NC_000006.12 6 4743548 - CDYL NNNCTGAATAATATAAAGCA NGC 2 0.0202 Tier II
21 NC_000007.14 7 146651797 - CNTNAP2 NNNCAGAATAATATATAGCA NTG 2 0.0195 Tier II
22 NC_000011.10 11 59799225 + STX3 NNNCTGAATAAAATATAGCA NGC 2 0.0178 Tier II
23 NC_000001.11 1 111629378 + RAP1A NNNCTGAATAATATAAAGCA NGT 2 0.0147 Tier II
24 NC_000001.11 1 99922814 - AGL NNNCTGAATATTATATAGCA NTG 2 0.012 Tier II
25 NC_000004.12 4 56474040 - SRP72 NNNCTGAATAATAAATAGCA NGT 2 0.01 Tier II
26 NC_000006.12 6 116651797 + ZUP1 NNNCTGAATAATATTTAGCA NTG 2 0.0078 Tier II
27 NC_000005.10 5 16131080 + MARCHF11 NNNCTGAATAATAGATAGAA NGG 2 0.0 Tier II
28 NC_000016.10 16 77315239 + ADAMTS18 NNNCTGAATAATAGATTGCA NGG 2 0.0 Tier II
29 NC_000011.10 11 89327946 + NOX4 NNNCTGAATAATAGGTAGCA NGG 2 0.0 Tier II
30 NC_000015.10 15 76621518 + SCAPER NNNCTGAATAATATATAGCA NAA 2 0.0 Tier II
31 NC_000017.11 17 29204274 + TWF1P1 NNNCTGAATAATATATAGCA NGG 0 1.0 Tier III
32 NC_000024.10 Y 19457132 + BCORP1 NNNCTGAATTATATAAAGCA NGG 2 0.8021 Tier III
33 NC_000016.10 16 51509113 + LOC102723323 NNNCTGAATTATATATAGAA NGG 2 0.3782 Tier III
34 NC_000023.11 X 155291176 + TWF1P2 NNNCTGAATAATATATAGCA NAG 1 0.2593 Tier III
35 NC_000004.12 4 15183767 + C1QTNF7-AS1 NNNCTGATTAATATATAGCT NGG 2 0.2571 Tier III
36 NC_000001.11 1 13690477 + LOC105376756 NNNCTGAATAATCTATAGCA NGG 1 0.2105 Tier III
37 NC_000003.12 3 109805674 + LOC124906267 NNNCTGAATAATAAATAGCA NAG 2 0.1605 Tier III
38 NC_000004.12 4 13806856 - LOC101929048 NNNCTGAATAATATAGAGAA NGG 2 0.1484 Tier III
39 NC_000004.12 4 13806856 - LINC01182 NNNCTGAATAATATAGAGAA NGG 2 0.1484 Tier III
40 NC_000006.12 6 77108636 + LOC101928570 NNNCTGAATAATTTAGAGCA NGG 2 0.1038 Tier III
41 NC_000004.12 4 136821839 + LINC02511 NNNCTGAATAATATATAGAA NTG 2 0.0167 Tier III
42 NC_000001.11 1 166291179 + LOC112268276 NNNCTGAATAACATATAGCA NGT 2 0.0153 Tier III
43 NC_000001.11 1 99922814 - LOC124904230 NNNCTGAATATTATATAGCA NTG 2 0.012 Tier III
44 NC_000005.10 5 16131080 + MARCHF11-AS1 NNNCTGAATAATAGATAGAA NGG 2 0.0 Tier III
45 NC_000011.10 11 49378301 - NOX4P1 NNNCTGAATAATAGGTAGCA NGG 2 0.0 Tier III
46 NC_000013.11 13 73565043 + LINC00392 NNNCTGAATTATAGATAGCA NGG 2 0.0 Tier III
47 NC_000013.11 13 73565043 + LINC00393 NNNCTGAATTATAGATAGCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 94586439 + Twf1 NNNCTGAATAATACATAGCA NGG 1 0.2857 Tier I
2 NC_000078.6 12 118265902 + Sp4 NNNATGAATAATATAAAGCA NGG 2 0.7656 Tier II
3 NC_000077.6 11 61961518 + Specc1 NNNCTGAAAAATATATATCA NGG 2 0.619 Tier II
4 NC_000069.6 3 152740067 + Pigk NNNCTGAGTAATATATAGCT NGG 2 0.44 Tier II
5 NC_000075.6 9 61379139 - Tle3 NNNCTGGATAATAAATAGCA NGG 2 0.437 Tier II
6 NC_000068.7 2 16593524 + Plxdc2 NNNCTGAAGAATATATAACA NGG 2 0.4286 Tier II
7 NC_000074.6 8 108922932 + Zfhx3 NNNCTGAAGAATATATAACA NGG 2 0.4286 Tier II
8 NC_000073.6 7 90794475 - Dlg2 NNNCTGAATTATATATAGTA NGG 2 0.4072 Tier II
9 NC_000071.6 5 30018826 - Il6 NNNCTGAATACTATATAGCA NGG 1 0.4 Tier II
10 NC_000080.6 14 30805834 + Sfmbt1 NNNCTGTATTATATATAGCA NGG 2 0.386 Tier II
11 NC_000082.6 16 22246209 - Tra2b NNNCTGTATTATATATAGCA NGG 2 0.386 Tier II
12 NC_000067.6 1 25818804 - Adgrb3 NNNATGTATAATATATAGCA NGG 2 0.3684 Tier II
13 NC_000071.6 5 65127113 + Klhl5 NNNATGTATAATATATAGCA NGG 2 0.3684 Tier II
14 NC_000075.6 9 29500351 + Ntm NNNCTGAAAATTATATAGCA NGG 2 0.2857 Tier II
15 NC_000067.6 1 154848894 + Cacna1e NNNCTGAGTAATACATAGCA NGG 2 0.2095 Tier II
16 NC_000074.6 8 95853001 + Slc38a7 NNNCTGAATAACCTATAGCA NGG 2 0.1994 Tier II
17 NC_000082.6 16 97070209 + Dscam NNNCTGAATAAACTATAGCA NGG 2 0.1684 Tier II
18 NC_000070.6 4 84944166 + Cntln NNNTTGAATAATATATGGCA NGG 2 0.1412 Tier II
19 NC_000081.6 15 76494082 - Hsf1 NNNCTGCATAATATATAGCA NAG 2 0.1134 Tier II
20 NC_000076.6 10 107623686 + Ptprq NNNCTGAAGAATATATAGGA NGG 2 0.0774 Tier II
21 NC_000071.6 5 120744402 + Oas2 NNNCAGAATAATATATAGGA NGG 2 0.0625 Tier II
22 NC_000080.6 14 117404566 + Gpc6 NNNCTGCATAATATATTGCA NGG 2 0.0583 Tier II
23 NC_000080.6 14 21632698 + Kat6b NNNCTGAATAATATATTGCA NAG 2 0.0346 Tier II
24 NC_000082.6 16 70458001 - Gbe1 NNNCTGAATAATATGTAGCA NTG 2 0.0253 Tier II
25 NC_000072.6 6 113105784 - Setd5 NNNCTGAATAATATATAGAA NTG 2 0.0167 Tier II
26 NC_000069.6 3 90061624 - 4933434E20Rik NNNCTGAAGAATATATAGCA NGC 2 0.0138 Tier II
27 NC_000070.6 4 104583988 - Dab1 NNNCTGAATAACAGATAGCA NGG 2 0.0 Tier II
28 NC_000071.6 5 30018826 - A230098N10Rik NNNCTGAATACTATATAGCA NGG 1 0.4 Tier III
29 NC_000069.6 3 150514591 - Gm6439 NNNCTGTATTATATATAGCA NGG 2 0.386 Tier III
30 NC_000086.7 X 56154885 - Gm14639 NNNCTGTATTATATATAGCA NGG 2 0.386 Tier III
31 NC_000069.6 3 139143767 + Gm4862 NNNCTGAATAATATATAGTG NGG 2 0.3529 Tier III
32 NC_000071.6 5 148795495 + Gm36405 NNNCTGAATAAAATATAGAA NGG 2 0.3429 Tier III
33 NC_000073.6 7 104821405 - Gm4887 NNNCTGAATAATACATAGCA NGG 1 0.2857 Tier III
34 NC_000078.6 12 97350264 - Gm40552 NNNCTGAATAATATATACCT NGG 2 0.2857 Tier III
35 NC_000076.6 10 107623686 + Gm36177 NNNCTGAAGAATATATAGGA NGG 2 0.0774 Tier III
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Other clones with same target sequence:

(none)