Construct: sgRNA BRDN0001149027
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCTTACTATCCCATAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAG1 (5571)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77748
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
49005492 |
+ |
PRKAG1 |
NNNCTTACTATCCCATAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
29075287 |
- |
LINGO2 |
NNNCTTATTATTCCATAAAG |
NGG |
2 |
0.4712 |
Tier II |
| 3 |
NC_000003.12 |
3 |
37593409 |
+ |
ITGA9 |
NNNCTGACTATCCCATAAAT |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000005.10 |
5 |
96092659 |
+ |
CAST |
NNNCATACTATCTCATAAAG |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000008.11 |
8 |
27830938 |
+ |
PBK |
NNNATTACTATCACATAAAG |
NGG |
2 |
0.3239 |
Tier II |
| 6 |
NC_000009.12 |
9 |
132525010 |
- |
CFAP77 |
NNNATTACTATCACATAAAG |
NGG |
2 |
0.3239 |
Tier II |
| 7 |
NC_000003.12 |
3 |
171714557 |
- |
PLD1 |
NNNCTTACTATTCCATAATG |
NGG |
2 |
0.2899 |
Tier II |
| 8 |
NC_000007.14 |
7 |
40382429 |
- |
SUGCT |
NNNCTTCCTATCCAATAAAG |
NGG |
2 |
0.1531 |
Tier II |
| 9 |
NC_000014.9 |
14 |
80730837 |
- |
CEP128 |
NNNCTTTCTCTCCCATAAAG |
NGG |
2 |
0.1458 |
Tier II |
| 10 |
NC_000007.14 |
7 |
134147740 |
+ |
LRGUK |
NNNCTTACTATCCCTCAAAG |
NGG |
2 |
0.1333 |
Tier II |
| 11 |
NC_000012.12 |
12 |
49005492 |
+ |
DDN-AS1 |
NNNCTTACTATCCCATAAAG |
NGG |
0 |
1.0 |
Tier III |
| 12 |
NC_000001.11 |
1 |
56058781 |
+ |
LOC105378737 |
NNNCTTAGTTTCCCATAAAG |
NGG |
2 |
0.5672 |
Tier III |
| 13 |
NC_000001.11 |
1 |
56058781 |
+ |
LOC105378741 |
NNNCTTAGTTTCCCATAAAG |
NGG |
2 |
0.5672 |
Tier III |
| 14 |
NC_000013.11 |
13 |
66027262 |
- |
LOC105370245 |
NNNCTTACTATTCTATAAAG |
NGG |
2 |
0.3949 |
Tier III |
| 15 |
NC_000005.10 |
5 |
96092659 |
+ |
LOC101929710 |
NNNCATACTATCTCATAAAG |
NGG |
2 |
0.35 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
98815689 |
+ |
Prkag1 |
NNNCTTACTGTCCCACAAAG |
NGG |
2 |
0.3704 |
Tier I |
| 2 |
NC_000071.6 |
5 |
89166929 |
- |
Slc4a4 |
NNNCTAACCATCCCATAAAG |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000076.6 |
10 |
28791723 |
+ |
Themis |
NNNCCTACTACCCCATAAAG |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000077.6 |
11 |
95142696 |
- |
Dlx4 |
NNNCTCAGTATCCCATAAAG |
NGG |
2 |
0.5844 |
Tier II |
| 5 |
NC_000084.6 |
18 |
15659416 |
+ |
Chst9 |
NNNCTTAGTATCCTATAAAG |
NGG |
2 |
0.4714 |
Tier II |
| 6 |
NC_000075.6 |
9 |
60210715 |
+ |
Thsd4 |
NNNCTTACTAGCCCATAAAA |
NGG |
2 |
0.375 |
Tier II |
| 7 |
NC_000071.6 |
5 |
146428402 |
+ |
Wasf3 |
NNNCTTCCTCTCCCATAAAG |
NGG |
2 |
0.1458 |
Tier II |
| 8 |
NC_000078.6 |
12 |
111862668 |
- |
Ppp1r13b |
NNNCTTACTATCCTATGAAG |
NGG |
2 |
0.1294 |
Tier II |
| 9 |
NC_000081.6 |
15 |
11240950 |
- |
Adamts12 |
NNNCCTACTATCCCATAAAG |
NGA |
2 |
0.0694 |
Tier II |
| 10 |
NC_000076.6 |
10 |
59751094 |
+ |
Micu1 |
NNNTTTACTATCCCATAAAG |
NTG |
2 |
0.0312 |
Tier II |
| 11 |
NC_000068.7 |
2 |
162411648 |
+ |
Ptprt |
NNNCTTACTATCCCATGAAG |
NTG |
2 |
0.0069 |
Tier II |
| 12 |
NC_000072.6 |
6 |
31670652 |
- |
Gm13847 |
NNNCTTACCATCCCACAAAG |
NGG |
2 |
0.6154 |
Tier III |
| 13 |
NC_000072.6 |
6 |
31670652 |
- |
Gm13846 |
NNNCTTACCATCCCACAAAG |
NGG |
2 |
0.6154 |
Tier III |
| 14 |
NC_000076.6 |
10 |
84057354 |
+ |
Gm34184 |
NNNCTTACCATCCCATACAG |
NGG |
2 |
0.1758 |
Tier III |
| 15 |
NC_000067.6 |
1 |
161899370 |
+ |
Gm31925 |
NNNCTTCCTATCCCAGAAAG |
NGG |
2 |
0.1514 |
Tier III |
Other clones with same target sequence:
(none)
