Construct: sgRNA BRDN0001149031
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGCCTATGACATAGCAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TESK2 (10420)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76433
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
45355361 |
- |
TESK2 |
NNNGCCTATGACATAGCAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
70143910 |
- |
TYSND1 |
NNNCCCTATGACATAGCAGT |
NGT |
2 |
0.0085 |
Tier I |
| 3 |
NC_000003.12 |
3 |
51141358 |
+ |
DOCK3 |
NNNCCCTATTACATAGCAGT |
NGG |
2 |
0.4301 |
Tier II |
| 4 |
NC_000015.10 |
15 |
101306922 |
+ |
PCSK6 |
NNNGCCTATGGCATGGCAGT |
NGG |
2 |
0.4225 |
Tier II |
| 5 |
NC_000002.12 |
2 |
144512528 |
+ |
ZEB2 |
NNNACCTATGACATAGCAGT |
NGA |
2 |
0.0625 |
Tier II |
| 6 |
NC_000016.10 |
16 |
25951102 |
- |
HS3ST4 |
NNNGCCTATGACCTAGCAGC |
NGG |
2 |
0.0191 |
Tier II |
| 7 |
NC_000008.11 |
8 |
125154094 |
- |
NSMCE2 |
NNNGTCTATGACAGAGCAGT |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
116792177 |
+ |
Tesk2 |
NNNGCCTATGACATAGCAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
49180660 |
- |
Btnl9 |
NNNGCCTATGACATTGCAGA |
NGG |
2 |
0.1125 |
Tier I |
| 3 |
NC_000083.6 |
17 |
86000327 |
- |
Srbd1 |
NNNTCCTATGACATAGCAGT |
NAG |
2 |
0.0943 |
Tier I |
| 4 |
NC_000072.6 |
6 |
99278492 |
- |
Foxp1 |
NNNGCCAATGACATAACAGT |
NGG |
2 |
0.875 |
Tier II |
| 5 |
NC_000072.6 |
6 |
138404810 |
+ |
Lmo3 |
NNNGCCTATGACATAGTACT |
NGG |
2 |
0.2092 |
Tier II |
| 6 |
NC_000070.6 |
4 |
154147299 |
- |
Wrap73 |
NNNGGCTATGACCTAGCAGT |
NGG |
2 |
0.1263 |
Tier II |
| 7 |
NC_000085.6 |
19 |
42395674 |
- |
Crtac1 |
NNNGCCTATGTCATAGCGGT |
NGG |
2 |
0.1231 |
Tier II |
| 8 |
NC_000080.6 |
14 |
69963951 |
- |
Pebp4 |
NNNGCCTCTGACACAGCAGT |
NGG |
2 |
0.1224 |
Tier II |
| 9 |
NC_000075.6 |
9 |
37244363 |
+ |
Slc37a2 |
NNNGCCTATGCCATAGCAGC |
NGG |
2 |
0.0364 |
Tier II |
| 10 |
NC_000074.6 |
8 |
118292798 |
- |
Cdh13 |
NNNGCCTATGACTTAGCAGT |
NGA |
2 |
0.0208 |
Tier II |
| 11 |
NC_000076.6 |
10 |
41567218 |
+ |
Ccdc162 |
NNNGCCTATGACCTAGCAGC |
NGG |
2 |
0.0191 |
Tier II |
| 12 |
NC_000078.6 |
12 |
41279353 |
+ |
Immp2l |
NNNGCCTATGCCATAGCAGT |
NTG |
2 |
0.0156 |
Tier II |
| 13 |
NC_000074.6 |
8 |
70727247 |
+ |
Pde4c |
NNNGCATATGACAGAGCAGT |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000069.6 |
3 |
84013797 |
- |
Tmem131l |
NNNGCCTATGACAGAGCAGT |
NAG |
2 |
0.0 |
Tier II |
| 15 |
NC_000085.6 |
19 |
42395674 |
- |
Gm41848 |
NNNGCCTATGTCATAGCGGT |
NGG |
2 |
0.1231 |
Tier III |
| 16 |
NC_000072.6 |
6 |
22617301 |
- |
Gm38774 |
NNNTCCTATGACACAGCAGT |
NGG |
2 |
0.1039 |
Tier III |
| 17 |
NC_000074.6 |
8 |
118292798 |
- |
Gm51555 |
NNNGCCTATGACTTAGCAGT |
NGA |
2 |
0.0208 |
Tier III |
Other clones with same target sequence:
(none)
