Construct: sgRNA BRDN0001149032

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGGGTCCCACACAACTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHKG2 (5261)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77385

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 30756182 - PHKG2 NNNGGTCCCACACAACTCTG NGG 0 1.0 Tier I
2 NC_000010.11 10 58337595 - UBE2D1 NNNGGTTCCACACAACTCTA NGG 2 0.7617 Tier II
3 NC_000005.10 5 146993944 - PPP2R2B NNNGGTCACACACAGCTCTG NGG 2 0.4225 Tier II
4 NC_000004.12 4 7849724 + AFAP1 NNNTGCCCCACACAACTCTG NGG 2 0.3306 Tier II
5 NC_000016.10 16 85287356 - GSE1 NNNGGTCCCACAAAACCCTG NGG 2 0.2715 Tier II
6 NC_000017.11 17 60491296 - APPBP2 NNNGGTTCCACACATCTCTG NGG 2 0.1625 Tier II
7 NC_000017.11 17 73592984 - SDK2 NNNCGTCCCACACCACTCTG NGG 2 0.1134 Tier II
8 NC_000001.11 1 176862967 - ASTN1 NNNTGTCCCACACAACTCAG NGG 2 0.1039 Tier II
9 NC_000004.12 4 84644091 - CDS1 NNNGGTCCCACCAAACTCTG NGG 2 0.1012 Tier II
10 NC_000022.11 22 20750683 + PI4KA NNNGGTGCCACACCACTCTG NGG 2 0.1008 Tier II
11 NC_000019.10 19 49302316 + SLC6A16 NNNGGTCCCAGAAAACTCTG NGG 2 0.0962 Tier II
12 NC_000001.11 1 2099147 - PRKCZ NNNGGTCCCACACAGCTGTG NGG 2 0.0867 Tier II
13 NC_000012.12 12 11792375 + ETV6 NNNGGACCCACACAACTCTG NGA 2 0.0602 Tier II
14 NC_000005.10 5 150369333 - TCOF1 NNNGTTCCCACACATCTCTG NGG 2 0.06 Tier II
15 NC_000016.10 16 16111670 - ABCC1 NNNGGTCCCACAAAACGCTG NGG 2 0.0452 Tier II
16 NC_000023.11 X 78309585 + CYSLTR1 NNNGTTCCCACACAACTGTG NGG 2 0.04 Tier II
17 NC_000010.11 10 1556733 - ADARB2 NNNGCTCCCACACAACTCTG NTG 2 0.0306 Tier II
18 NC_000015.10 15 85453066 - AKAP13 NNNGGTCCCACACACCTCTG NCG 2 0.0292 Tier II
19 NC_000003.12 3 12949149 - IQSEC1 NNNGGTCCCACACAACTCTG NCT 2 0.0 Tier II
20 NC_000001.11 1 63723244 + LOC105378771 NNNGGTCCTACACAACACTG NGG 2 0.4667 Tier III
21 NC_000016.10 16 85287356 - LINC00311 NNNGGTCCCACAAAACCCTG NGG 2 0.2715 Tier III
22 NC_000002.12 2 223515448 + LOC105373907 NNNGCTCCCACACAACTCTG NAG 2 0.2037 Tier III
23 NC_000001.11 1 221305746 + LOC105372932 NNNGGTCCCACACCACCCTG NGG 2 0.1513 Tier III
24 NC_000004.12 4 174384085 + LOC105377547 NNNGGACCCACAGAACTCTG NGG 2 0.1182 Tier III
25 NC_000006.12 6 90749409 - LOC107986623 NNNGTTCCCACAGAACTCTG NGG 2 0.0409 Tier III
26 NC_000010.11 10 1556733 - ADARB2-AS1 NNNGCTCCCACACAACTCTG NTG 2 0.0306 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 129016375 - Zc3h6 NNNGGTCCCACACAAGTCTA NGG 2 0.1442 Tier I
2 NC_000067.6 1 84804501 - Trip12 NNNGGAACCACACAACTCTG NGG 2 0.65 Tier II
3 NC_000072.6 6 37895611 + Trim24 NNNGGAACCACACAACTCTG NGG 2 0.65 Tier II
4 NC_000075.6 9 35487461 + Cdon NNNGGACCCAAACAACTCTG NGG 2 0.65 Tier II
5 NC_000068.7 2 76280951 + Pde11a NNNAGTCACACACAACTCTG NGG 2 0.585 Tier II
6 NC_000078.6 12 91752968 + Ston2 NNNGGTGTCACACAACTCTG NGG 2 0.4118 Tier II
7 NC_000068.7 2 5435268 - Camk1d NNNGGGCCCACACAGCTCTG NGG 2 0.3714 Tier II
8 NC_000075.6 9 53303801 + Exph5 NNNTGTCCCTCACAACTCTG NGG 2 0.3209 Tier II
9 NC_000085.6 19 41184030 - Tll2 NNNTGTCTCACACAACTCTG NGG 2 0.3182 Tier II
10 NC_000071.6 5 8493909 + Rundc3b NNNGGGCCCACACAACACTG NGG 2 0.3048 Tier II
11 NC_000072.6 6 56718827 - Avl9 NNNGGTCCCACACACCTCTG NGG 1 0.2727 Tier II
12 NC_000069.6 3 121230881 + Tlcd4 NNNGGTCCCACACAACTCTG NAG 1 0.2593 Tier II
13 NC_000067.6 1 81128023 - Nyap2 NNNGGTCCCACCCAACTTTG NGG 2 0.1692 Tier II
14 NC_000068.7 2 112394548 - Katnbl1 NNNCTTCCCACACAACTCTG NGG 2 0.1588 Tier II
15 NC_000068.7 2 28534136 - Ralgds NNNGGTCCCACTCACCTCTG NGG 2 0.0909 Tier II
16 NC_000076.6 10 7806909 - Ppil4 NNNGGTCCCACAGAGCTCTG NGG 2 0.0886 Tier II
17 NC_000074.6 8 24012465 + Zmat4 NNNGTTCCCACACCACTCTG NGG 2 0.0643 Tier II
18 NC_000077.6 11 74843758 - Mnt NNNGGTCCCACAGGACTCTG NGG 2 0.0636 Tier II
19 NC_000077.6 11 98767551 - Thra NNNGGTCCCACCCATCTCTG NGG 2 0.0526 Tier II
20 NC_000067.6 1 64578664 - Creb1 NNNGGTCCCACACCTCTCTG NGG 2 0.0429 Tier II
21 NC_000069.6 3 122211068 - Gm46822 NNNGGTCCTACACAACTCTG NGG 1 0.875 Tier III
22 NC_000070.6 4 93443819 - Gm31828 NNNGGTCCCACACTGCTCTG NGG 2 0.3467 Tier III
23 NC_000077.6 11 114225180 + Gm11690 NNNGGTCCCACCCAACTCTT NGG 2 0.1842 Tier III
24 NC_000081.6 15 99260217 - 1700120C14Rik NNNGCTCCCACACAACTCTG NGC 2 0.0175 Tier III
25 NC_000078.6 12 43793143 - Gm33384 NNNGGTCCCACACAACACTG NGC 2 0.0119 Tier III
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Other clones with same target sequence:

(none)