Construct: sgRNA BRDN0001149032
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGGGTCCCACACAACTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKG2 (5261)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77385
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000068.7 |
2 |
129016375 |
- |
Zc3h6 |
NNNGGTCCCACACAAGTCTA |
NGG |
2 |
0.1442 |
Tier I |
2 |
NC_000067.6 |
1 |
84804501 |
- |
Trip12 |
NNNGGAACCACACAACTCTG |
NGG |
2 |
0.65 |
Tier II |
3 |
NC_000072.6 |
6 |
37895611 |
+ |
Trim24 |
NNNGGAACCACACAACTCTG |
NGG |
2 |
0.65 |
Tier II |
4 |
NC_000075.6 |
9 |
35487461 |
+ |
Cdon |
NNNGGACCCAAACAACTCTG |
NGG |
2 |
0.65 |
Tier II |
5 |
NC_000068.7 |
2 |
76280951 |
+ |
Pde11a |
NNNAGTCACACACAACTCTG |
NGG |
2 |
0.585 |
Tier II |
6 |
NC_000078.6 |
12 |
91752968 |
+ |
Ston2 |
NNNGGTGTCACACAACTCTG |
NGG |
2 |
0.4118 |
Tier II |
7 |
NC_000068.7 |
2 |
5435268 |
- |
Camk1d |
NNNGGGCCCACACAGCTCTG |
NGG |
2 |
0.3714 |
Tier II |
8 |
NC_000075.6 |
9 |
53303801 |
+ |
Exph5 |
NNNTGTCCCTCACAACTCTG |
NGG |
2 |
0.3209 |
Tier II |
9 |
NC_000085.6 |
19 |
41184030 |
- |
Tll2 |
NNNTGTCTCACACAACTCTG |
NGG |
2 |
0.3182 |
Tier II |
10 |
NC_000071.6 |
5 |
8493909 |
+ |
Rundc3b |
NNNGGGCCCACACAACACTG |
NGG |
2 |
0.3048 |
Tier II |
11 |
NC_000072.6 |
6 |
56718827 |
- |
Avl9 |
NNNGGTCCCACACACCTCTG |
NGG |
1 |
0.2727 |
Tier II |
12 |
NC_000069.6 |
3 |
121230881 |
+ |
Tlcd4 |
NNNGGTCCCACACAACTCTG |
NAG |
1 |
0.2593 |
Tier II |
13 |
NC_000067.6 |
1 |
81128023 |
- |
Nyap2 |
NNNGGTCCCACCCAACTTTG |
NGG |
2 |
0.1692 |
Tier II |
14 |
NC_000068.7 |
2 |
112394548 |
- |
Katnbl1 |
NNNCTTCCCACACAACTCTG |
NGG |
2 |
0.1588 |
Tier II |
15 |
NC_000068.7 |
2 |
28534136 |
- |
Ralgds |
NNNGGTCCCACTCACCTCTG |
NGG |
2 |
0.0909 |
Tier II |
16 |
NC_000076.6 |
10 |
7806909 |
- |
Ppil4 |
NNNGGTCCCACAGAGCTCTG |
NGG |
2 |
0.0886 |
Tier II |
17 |
NC_000074.6 |
8 |
24012465 |
+ |
Zmat4 |
NNNGTTCCCACACCACTCTG |
NGG |
2 |
0.0643 |
Tier II |
18 |
NC_000077.6 |
11 |
74843758 |
- |
Mnt |
NNNGGTCCCACAGGACTCTG |
NGG |
2 |
0.0636 |
Tier II |
19 |
NC_000077.6 |
11 |
98767551 |
- |
Thra |
NNNGGTCCCACCCATCTCTG |
NGG |
2 |
0.0526 |
Tier II |
20 |
NC_000067.6 |
1 |
64578664 |
- |
Creb1 |
NNNGGTCCCACACCTCTCTG |
NGG |
2 |
0.0429 |
Tier II |
21 |
NC_000069.6 |
3 |
122211068 |
- |
Gm46822 |
NNNGGTCCTACACAACTCTG |
NGG |
1 |
0.875 |
Tier III |
22 |
NC_000070.6 |
4 |
93443819 |
- |
Gm31828 |
NNNGGTCCCACACTGCTCTG |
NGG |
2 |
0.3467 |
Tier III |
23 |
NC_000077.6 |
11 |
114225180 |
+ |
Gm11690 |
NNNGGTCCCACCCAACTCTT |
NGG |
2 |
0.1842 |
Tier III |
24 |
NC_000081.6 |
15 |
99260217 |
- |
1700120C14Rik |
NNNGCTCCCACACAACTCTG |
NGC |
2 |
0.0175 |
Tier III |
25 |
NC_000078.6 |
12 |
43793143 |
- |
Gm33384 |
NNNGGTCCCACACAACACTG |
NGC |
2 |
0.0119 |
Tier III |
Other clones with same target sequence:
(none)