Construct: sgRNA BRDN0001149033
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACCTACCTGAATAACACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ATM (472)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77531
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
108299870 |
+ |
ATM |
NNNCTACCTGAATAACACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
165823022 |
- |
BCHE |
NNNTTACCTAAATAACACAC |
NGG |
2 |
0.7467 |
Tier II |
| 3 |
NC_000006.12 |
6 |
142858357 |
+ |
HIVEP2 |
NNNCTACCTAATTAACACAC |
NGG |
2 |
0.3111 |
Tier II |
| 4 |
NC_000007.14 |
7 |
116706287 |
- |
MET |
NNNCTACCTAAATAAGACAC |
NGG |
2 |
0.1436 |
Tier II |
| 5 |
NC_000023.11 |
X |
132808154 |
+ |
HS6ST2 |
NNNCTATCTGAATAAGACAC |
NGG |
2 |
0.125 |
Tier II |
| 6 |
NC_000004.12 |
4 |
76766621 |
- |
SHROOM3 |
NNNCTACCAGAATAACTCAC |
NGG |
2 |
0.1238 |
Tier II |
| 7 |
NC_000014.9 |
14 |
90759783 |
- |
TTC7B |
NNNCTAGCTGAATAACACAC |
NAG |
2 |
0.122 |
Tier II |
| 8 |
NC_000010.11 |
10 |
28607732 |
- |
WAC |
NNNCTACCTCAATAAGACAC |
NGG |
2 |
0.0615 |
Tier II |
| 9 |
NC_000008.11 |
8 |
13226328 |
- |
DLC1 |
NNNCTACCTGAATATTACAC |
NGG |
2 |
0.0615 |
Tier II |
| 10 |
NC_000009.12 |
9 |
133806337 |
- |
VAV2 |
NNNCTACCTGAATCACACCC |
NGG |
2 |
0.0443 |
Tier II |
| 11 |
NC_000011.10 |
11 |
40547672 |
+ |
LRRC4C |
NNNCTACCTGAATAACACAA |
NGA |
2 |
0.0347 |
Tier II |
| 12 |
NC_000019.10 |
19 |
56351447 |
+ |
ZSCAN5A |
NNNCTACATGAATAACACAC |
NTG |
2 |
0.0253 |
Tier II |
| 13 |
NC_000008.11 |
8 |
107398155 |
+ |
ANGPT1 |
NNNCTACCTGAATAACCCAC |
NGT |
2 |
0.0028 |
Tier II |
| 14 |
NC_000009.12 |
9 |
80890590 |
+ |
LOC107987085 |
NNNCTACATAAATAACACAC |
NGG |
2 |
0.6067 |
Tier III |
| 15 |
NC_000004.12 |
4 |
76766621 |
- |
SHROOM3-AS1 |
NNNCTACCAGAATAACTCAC |
NGG |
2 |
0.1238 |
Tier III |
| 16 |
NC_000018.10 |
18 |
39214834 |
- |
MIR924HG |
NNNCTACCTGAATATCACAT |
NGG |
2 |
0.06 |
Tier III |
| 17 |
NC_000016.10 |
16 |
35096789 |
- |
VPS35P1 |
NNNCTACCTGAATAACACAC |
NTC |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
6294970 |
+ |
Tmem181a |
NNNCTACCTGAATTACACAC |
NGT |
2 |
0.0086 |
Tier I |
| 2 |
NC_000076.6 |
10 |
11300814 |
- |
Fbxo30 |
NNNTCACCTGAATAACACAC |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000080.6 |
14 |
23344640 |
- |
Kcnma1 |
NNNATACATGAATAACACAC |
NGG |
2 |
0.5474 |
Tier II |
| 4 |
NC_000075.6 |
9 |
42839648 |
- |
Grik4 |
NNNTTACCTGAATAACACTC |
NGG |
2 |
0.4308 |
Tier II |
| 5 |
NC_000069.6 |
3 |
41581496 |
- |
Jade1 |
NNNCTCCCTGGATAACACAC |
NGG |
2 |
0.2955 |
Tier II |
| 6 |
NC_000069.6 |
3 |
117588095 |
+ |
Plppr5 |
NNNCTACCTAAATAACACAC |
NAG |
2 |
0.242 |
Tier II |
| 7 |
NC_000072.6 |
6 |
110968918 |
- |
Grm7 |
NNNCTGCCTGTATAACACAC |
NGG |
2 |
0.2198 |
Tier II |
| 8 |
NC_000074.6 |
8 |
105741830 |
+ |
Gfod2 |
NNNCTACCTGAAGAACACAC |
NGA |
2 |
0.0181 |
Tier II |
| 9 |
NC_000067.6 |
1 |
184924362 |
+ |
Mark1 |
NNNCTACCTGAATAACAGAC |
NGA |
2 |
0.0093 |
Tier II |
| 10 |
NC_000076.6 |
10 |
25633960 |
- |
Gm29571 |
NNNCTACCTCAATAACACTC |
NGG |
2 |
0.2154 |
Tier III |
Other clones with same target sequence:
(none)
