Construct: sgRNA BRDN0001149034

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAATCCTTCTGGTACACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PANK4 (55229)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76968

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 2520827 + PANK4 NNNATCCTTCTGGTACACGA NGG 0 1.0 Tier I
2 NC_000012.12 12 106072819 + NUAK1 NNNATCCTTCTGGTACAGGT NGG 2 0.08 Tier I
3 NC_000006.12 6 161941533 + PRKN NNNATTCTTCTGGTACACAA NGG 2 0.6633 Tier II
4 NC_000007.14 7 100058959 - ZSCAN21 NNNATCCTTCTGGTAAATGA NGG 2 0.6429 Tier II
5 NC_000005.10 5 96924130 + ERAP1 NNNATCCTGCTAGTACACGA NGG 2 0.5778 Tier II
6 NC_000017.11 17 79940169 - TBC1D16 NNNGTCCTCCTGGTACACGA NGG 2 0.5769 Tier II
7 NC_000011.10 11 119009618 - CENATAC NNNATCCTCCTGGAACACGA NGG 2 0.5714 Tier II
8 NC_000005.10 5 31466092 - DROSHA NNNATACTTCTGGTACAAGA NGG 2 0.5 Tier II
9 NC_000001.11 1 93728501 - BCAR3 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
10 NC_000001.11 1 227754035 - SNAP47 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
11 NC_000002.12 2 98172165 - VWA3B NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
12 NC_000002.12 2 154191944 - GALNT13 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
13 NC_000002.12 2 233766121 - UGT1A10 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
14 NC_000002.12 2 233766121 - UGT1A8 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
15 NC_000002.12 2 233766121 - UGT1A7 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
16 NC_000002.12 2 233766121 - UGT1A6 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
17 NC_000002.12 2 233766121 - UGT1A5 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
18 NC_000002.12 2 233766121 - UGT1A9 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
19 NC_000002.12 2 233766121 - UGT1A4 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
20 NC_000002.12 2 233766121 - UGT1A1 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
21 NC_000002.12 2 233766121 - UGT1A3 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
22 NC_000004.12 4 56617753 - SPMAP2L NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
23 NC_000004.12 4 143351218 - GAB1 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
24 NC_000006.12 6 3396895 + SLC22A23 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
25 NC_000006.12 6 64999965 + EYS NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
26 NC_000006.12 6 107079359 + BEND3 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
27 NC_000006.12 6 111309230 - REV3L NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
28 NC_000006.12 6 162023336 - PRKN NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
29 NC_000007.14 7 15443136 + AGMO NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
30 NC_000008.11 8 41499407 - GOLGA7 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
31 NC_000009.12 9 34797381 - PHF24 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
32 NC_000010.11 10 67551480 + CTNNA3 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
33 NC_000011.10 11 86413004 - CCDC81 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
34 NC_000012.12 12 104828390 + SLC41A2 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
35 NC_000020.11 20 8005970 + TMX4 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
36 NC_000023.11 X 74496705 - SLC16A2 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
37 NC_000023.11 X 81229447 - SH3BGRL NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
38 NC_000023.11 X 86349096 - DACH2 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier II
39 NC_000007.14 7 129407567 + AHCYL2 NNNATCCTTCTGGTACACTA NGA 2 0.0463 Tier II
40 NC_000017.11 17 57001565 - SCPEP1 NNNATCCTTCTGGTTCACGA NGC 2 0.0044 Tier II
41 NC_000019.10 19 12000089 + ZNF433-AS1 NNNATTCTTCTGGTACACTA NGG 2 0.619 Tier III
42 NC_000001.11 1 23838377 + LOC105376861 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
43 NC_000002.12 2 233766121 - UGT1A NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
44 NC_000003.12 3 7959011 - LOC101927394 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
45 NC_000003.12 3 59385527 + CFAP20DC-DT NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
46 NC_000003.12 3 118595692 - LOC105374060 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
47 NC_000004.12 4 88454670 + LOC102723458 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
48 NC_000005.10 5 38797655 - OSMR-DT NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
49 NC_000005.10 5 72802568 + TNPO1-DT NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
50 NC_000005.10 5 169521988 + LOC105377714 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
51 NC_000005.10 5 169521988 + LOC105377715 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
52 NC_000008.11 8 126820799 - LOC105375751 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
53 NC_000008.11 8 126820799 - LOC105375753 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
54 NC_000010.11 10 65733707 + LINC01515 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
55 NC_000011.10 11 102664344 + MMP20-AS1 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
56 NC_000012.12 12 131484334 + LOC101929974 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
57 NC_000013.11 13 79882615 - LINC00382 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
58 NC_000017.11 17 44985909 + LOC112268183 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
59 NC_000018.10 18 39707881 - MIR924HG NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
60 NC_000021.9 21 15523954 + LOC105369302 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
61 NC_000022.11 22 19296658 - LOC105372859 NNNATTCTTCTGGTACACCA NGG 2 0.4163 Tier III
62 NC_000012.12 12 85456845 + LINC02820 NNNATGCTTCTGGTACACTA NGG 2 0.3333 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 22588368 + Dgkg NNNATCCTTCAGGTACACTA NGG 2 0.5 Tier I
2 NC_000083.6 17 36965470 - Znrd1as NNNATCCTACTGGTACAGGA NGG 2 0.1238 Tier I
3 NC_000071.6 5 121566820 + Aldh2 NNNAACCTTCTGGTACAAGA NGG 2 0.2692 Tier II
4 NC_000070.6 4 57657071 + Pakap NNNCTCCTTCAGGTACACGA NGG 2 0.2647 Tier II
5 NC_000081.6 15 77819683 - Myh9 NNNATCCTTCTGGTACCCAA NGG 2 0.1261 Tier II
6 NC_000072.6 6 140065833 - Gm45959 NNNATACTTCTGGTACATGA NGG 2 0.5969 Tier III
7 NC_000082.6 16 22588368 + Gm41433 NNNATCCTTCAGGTACACTA NGG 2 0.5 Tier III
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Other clones with same target sequence:

(none)