Construct: sgRNA BRDN0001149036
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGACTTCAACTGCTACGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR2B (93)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75486
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
38477490 |
+ |
ACVR2B |
NNNACTTCAACTGCTACGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
146881568 |
+ |
CNTNAP2 |
NNNACTTCAAATGCTACTAT |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000002.12 |
2 |
229249752 |
+ |
PID1 |
NNNACTTCAACTGCTATGAC |
NGG |
2 |
0.0424 |
Tier II |
| 4 |
NC_000014.9 |
14 |
67007586 |
+ |
GPHN |
NNNACTTCAACTGCTACCAT |
NTG |
2 |
0.0186 |
Tier II |
| 5 |
NC_000011.10 |
11 |
30287027 |
+ |
ARL14EP-DT |
NNNACTTCAACTGCTACTAT |
NGG |
1 |
0.6667 |
Tier III |
| 6 |
NC_000002.12 |
2 |
13126496 |
+ |
LOC105373436 |
NNNACTTCAACTGTTAGGAT |
NGG |
2 |
0.0431 |
Tier III |
| 7 |
NC_000002.12 |
2 |
13126496 |
+ |
LOC105373484 |
NNNACTTCAACTGTTAGGAT |
NGG |
2 |
0.0431 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
119427658 |
+ |
Acvr2b |
NNNACTTCAATTGCTACGAC |
NGG |
2 |
0.028 |
Tier I |
| 2 |
NC_000082.6 |
16 |
94992997 |
+ |
Kcnj6 |
NNNACTTCAACTGCCACGAA |
NGG |
2 |
0.1534 |
Tier II |
| 3 |
NC_000073.6 |
7 |
64630888 |
- |
Apba2 |
NNNACTTCAACTGCTGCCAT |
NGG |
2 |
0.0916 |
Tier II |
| 4 |
NC_000085.6 |
19 |
11778970 |
+ |
Stx3 |
NNNACTTCAGCTGCTAGGAT |
NGG |
2 |
0.0327 |
Tier II |
Other clones with same target sequence:
(none)
