Construct: sgRNA BRDN0001149037
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCACCTCTCACGAACACGT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- FLT4 (2324)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000005.10 | 5 | 180630567 | + | FLT4 | NNNACCTCTCACGAACACGT | NGG | 0 | 1.0 | Tier I |
| 2 | NC_000001.11 | 1 | 24092959 | + | MYOM3 | NNNACCTCTCACAAGCACGT | NGG | 2 | 0.6 | Tier I |
| 3 | NC_000001.11 | 1 | 32108400 | - | KPNA6 | NNNACCTCCCAGGAACACGT | NGG | 2 | 0.4103 | Tier II |
| 4 | NC_000001.11 | 1 | 6662458 | - | DNAJC11 | NNNACCTCTCACCAACACTT | NGG | 2 | 0.2807 | Tier II |
| 5 | NC_000016.10 | 16 | 15426418 | - | MPV17L-BMERB1 | NNNACCTCTCACTAACACTT | NGG | 2 | 0.2 | Tier II |
| 6 | NC_000022.11 | 22 | 48734748 | - | TAFA5 | NNNACCTCTCAGGAACACCT | NGG | 2 | 0.1992 | Tier II |
| 7 | NC_000001.11 | 1 | 219153901 | + | LYPLAL1-DT | NNNACCTCTCCCGAACACGT | NGC | 2 | 0.0089 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
No results found.