Construct: sgRNA BRDN0001149038
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAACATGGTATAGTACACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TBCK (93627)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75566
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
106262139 |
- |
TBCK |
NNNACATGGTATAGTACACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
5698231 |
+ |
SOX11 |
NNNACATAGTATAATACACA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000004.12 |
4 |
121820323 |
- |
CCNA2 |
NNNACATGATATAGTACACT |
NGG |
2 |
0.3857 |
Tier II |
| 4 |
NC_000008.11 |
8 |
13311292 |
- |
DLC1 |
NNNGAATGGTATAGTACACA |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000003.12 |
3 |
9137210 |
- |
SRGAP3 |
NNNACTTGGTATAGTACATA |
NGG |
2 |
0.3297 |
Tier II |
| 6 |
NC_000003.12 |
3 |
116401148 |
- |
LSAMP |
NNNACATGGTAAAGTACAGA |
NGG |
2 |
0.1 |
Tier II |
| 7 |
NC_000012.12 |
12 |
39349872 |
+ |
KIF21A |
NNNATATGGTATAGTACACA |
NGA |
2 |
0.0442 |
Tier II |
| 8 |
NC_000008.11 |
8 |
91966065 |
- |
RUNX1T1 |
NNNTCATGGTATAGTAGACA |
NGG |
2 |
0.0374 |
Tier II |
| 9 |
NC_000011.10 |
11 |
79019651 |
- |
TENM4 |
NNNACATGGTATAGAACACA |
NTG |
2 |
0.0226 |
Tier II |
| 10 |
NC_000001.11 |
1 |
200771031 |
- |
CAMSAP2 |
NNNACATGGTATAATCCACA |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
56435795 |
+ |
Ptprs |
NNNCCATGGTATAGTAGACA |
NGG |
2 |
0.0208 |
Tier I |
| 2 |
NC_000067.6 |
1 |
16502701 |
+ |
Stau2 |
NNNACATAGTACAGTACACA |
NGG |
2 |
0.9474 |
Tier II |
| 3 |
NC_000068.7 |
2 |
143608197 |
+ |
Pcsk2 |
NNNACATGGAATGGTACACA |
NGG |
2 |
0.559 |
Tier II |
| 4 |
NC_000082.6 |
16 |
8732332 |
- |
Usp7 |
NNNGCAAGGTATAGTACACA |
NGG |
2 |
0.5469 |
Tier II |
| 5 |
NC_000073.6 |
7 |
122172944 |
- |
Ern2 |
NNNACATGGCACAGTACACA |
NGG |
2 |
0.5053 |
Tier II |
| 6 |
NC_000068.7 |
2 |
33624090 |
- |
Lmx1b |
NNNACAAGGTCTAGTACACA |
NGG |
2 |
0.35 |
Tier II |
| 7 |
NC_000069.6 |
3 |
59154408 |
+ |
Med12l |
NNNACATGGTATAGAACATA |
NGG |
2 |
0.2672 |
Tier II |
| 8 |
NC_000083.6 |
17 |
33603502 |
- |
Myo1f |
NNNACATGGTACAGCACACA |
NGG |
2 |
0.2584 |
Tier II |
| 9 |
NC_000070.6 |
4 |
73729913 |
+ |
Rasef |
NNNACATGGTATACAACACA |
NGG |
2 |
0.2481 |
Tier II |
| 10 |
NC_000071.6 |
5 |
16325233 |
- |
Cacna2d1 |
NNNACATGGTAGACTACACA |
NGG |
2 |
0.2143 |
Tier II |
| 11 |
NC_000069.6 |
3 |
25594019 |
+ |
Nlgn1 |
NNNAGATGGTATAGTACACA |
NAG |
2 |
0.1556 |
Tier II |
| 12 |
NC_000075.6 |
9 |
67332170 |
+ |
Tln2 |
NNNACATGGCATAGTACACA |
NAG |
2 |
0.1383 |
Tier II |
| 13 |
NC_000070.6 |
4 |
77962081 |
- |
Ptprd |
NNNACCTGGTATTGTACACA |
NGG |
2 |
0.1364 |
Tier II |
| 14 |
NC_000073.6 |
7 |
97415051 |
- |
Thrsp |
NNNACATGGTATATTATACA |
NGG |
2 |
0.1244 |
Tier II |
| 15 |
NC_000082.6 |
16 |
50228355 |
- |
Bbx |
NNNACATGGTATAGTACAAA |
NAG |
2 |
0.1111 |
Tier II |
| 16 |
NC_000067.6 |
1 |
132735937 |
+ |
Nfasc |
NNNAAATGGTATAGTACACA |
NTG |
2 |
0.0223 |
Tier II |
| 17 |
NC_000080.6 |
14 |
7878573 |
+ |
Flnb |
NNNACATGGTATAATACACA |
NGT |
2 |
0.0121 |
Tier II |
| 18 |
NC_000074.6 |
8 |
106999160 |
- |
Sntb2 |
NNNACATGGTATAGTAGCCA |
NGG |
2 |
0.0112 |
Tier II |
| 19 |
NC_000074.6 |
8 |
126341790 |
+ |
Slc35f3 |
NNNACATGATATAGTACACA |
NGT |
2 |
0.0104 |
Tier II |
| 20 |
NC_000072.6 |
6 |
85290505 |
- |
Sfxn5 |
NNNACATGGTATAGTACAGA |
NGA |
2 |
0.0087 |
Tier II |
| 21 |
NC_000080.6 |
14 |
13264293 |
- |
Gm5087 |
NNNGCATGGTATAGTACAAA |
NGG |
2 |
0.2679 |
Tier III |
Other clones with same target sequence:
(none)
