Construct: sgRNA BRDN0001149039
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAAGGTACACAGCAAGTGG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- BRD3 (8019)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000009.12 | 9 | 134050577 | - | BRD3 | NNNAGGTACACAGCAAGTGG | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 33427797 | + | PHC2 | NNNAGGTACACAACAAATGG | NGG | 2 | 0.8615 | Tier II |
3 | NC_000006.12 | 6 | 162097465 | + | PRKN | NNNACATACACAGCAAGTGG | NGG | 2 | 0.7857 | Tier II |
4 | NC_000010.11 | 10 | 21798599 | - | DNAJC1 | NNNAGGTACAAAGCAAGTGA | NGG | 2 | 0.7031 | Tier II |
5 | NC_000011.10 | 11 | 68034324 | - | NDUFS8 | NNNAGCTACACAGCAAGTGG | NGG | 1 | 0.6818 | Tier II |
6 | NC_000007.14 | 7 | 140060706 | - | PARP12 | NNNAGGCACACAGCAAATGG | NGG | 2 | 0.6417 | Tier II |
7 | NC_000004.12 | 4 | 138200040 | - | SLC7A11 | NNNAGATAGACAGCAAGTGG | NGG | 2 | 0.619 | Tier II |
8 | NC_000010.11 | 10 | 80465260 | - | TSPAN14 | NNNAGGCACAAAGCAAGTGG | NGG | 2 | 0.5156 | Tier II |
9 | NC_000022.11 | 22 | 37810170 | + | GCAT | NNNAGGTACACAGTGAGTGG | NGG | 2 | 0.4767 | Tier II |
10 | NC_000003.12 | 3 | 14690483 | + | C3orf20 | NNNAGTCACACAGCAAGTGG | NGG | 2 | 0.4583 | Tier II |
11 | NC_000009.12 | 9 | 288388 | - | DOCK8 | NNNAGTCACACAGCAAGTGG | NGG | 2 | 0.4583 | Tier II |
12 | NC_000015.10 | 15 | 60542797 | - | RORA | NNNAGGTTCTCAGCAAGTGG | NGG | 2 | 0.3782 | Tier II |
13 | NC_000015.10 | 15 | 60543050 | - | RORA | NNNAGGTTCTCAGCAAGTGG | NGG | 2 | 0.3782 | Tier II |
14 | NC_000017.11 | 17 | 45128263 | + | PLCD3 | NNNTGGGACACAGCAAGTGG | NGG | 2 | 0.3743 | Tier II |
15 | NC_000017.11 | 17 | 80718084 | - | RPTOR | NNNAGGTACACAGCAAAGGG | NGG | 2 | 0.3111 | Tier II |
16 | NC_000021.9 | 21 | 45802309 | - | PCBP3 | NNNAGGAACACAGCAAGGGG | NGG | 2 | 0.2917 | Tier II |
17 | NC_000010.11 | 10 | 75094570 | - | DUSP13B | NNNATGTAAACAGCAAGTGG | NGG | 2 | 0.2571 | Tier II |
18 | NC_000001.11 | 1 | 33812228 | + | CSMD2 | NNNAGGGCCACAGCAAGTGG | NGG | 2 | 0.2521 | Tier II |
19 | NC_000018.10 | 18 | 8130818 | + | PTPRM | NNNATGTACACAGCAAGTAG | NGG | 2 | 0.2143 | Tier II |
20 | NC_000001.11 | 1 | 174365644 | - | RABGAP1L | NNNAGGAACACAGCAACTGG | NGG | 2 | 0.2059 | Tier II |
21 | NC_000003.12 | 3 | 54298261 | + | CACNA2D3 | NNNAGGTACAAAGCCAGTGG | NGG | 2 | 0.2045 | Tier II |
22 | NC_000005.10 | 5 | 64583920 | - | RGS7BP | NNNAGGTACACAGTAAGTGG | NAG | 2 | 0.1901 | Tier II |
23 | NC_000001.11 | 1 | 7927529 | + | TNFRSF9 | NNNAGGTCCACAGCAAGTGC | NGG | 2 | 0.1837 | Tier II |
24 | NC_000004.12 | 4 | 169164511 | + | SH3RF1 | NNNAAGTACACAGCAGGTGG | NGG | 2 | 0.1667 | Tier II |
25 | NC_000006.12 | 6 | 42103670 | - | C6orf132 | NNNAGGTACACAGCAGGTGT | NGG | 2 | 0.1346 | Tier II |
26 | NC_000011.10 | 11 | 113425399 | + | DRD2 | NNNAGGTAGACAGCAGGTGG | NGG | 2 | 0.119 | Tier II |
27 | NC_000004.12 | 4 | 152805833 | + | ARFIP1 | NNNAGGTTCACAGCTAGTGG | NGG | 2 | 0.0857 | Tier II |
28 | NC_000020.11 | 20 | 62152690 | + | SS18L1 | NNNAGGTACACACCAGGTGG | NGG | 2 | 0.081 | Tier II |
29 | NC_000016.10 | 16 | 14662466 | - | BFAR | NNNAGGTAAACAGCAAGTGG | NGA | 2 | 0.0595 | Tier II |
30 | NC_000013.11 | 13 | 51401761 | + | INTS6 | NNNAGGTACACAGTAAGTGG | NGA | 2 | 0.0509 | Tier II |
31 | NC_000009.12 | 9 | 120454134 | + | CDK5RAP2 | NNNAGGTACACAGCAAGTGA | NTG | 2 | 0.0365 | Tier II |
32 | NC_000019.10 | 19 | 54906112 | - | NCR1 | NNNAGATACACAGGAAGTGG | NGG | 2 | 0.0 | Tier II |
33 | NC_000016.10 | 16 | 73010065 | + | ZFHX3 | NNNAGGTACACAGCACCTGG | NGG | 2 | 0.0 | Tier II |
34 | NC_000005.10 | 5 | 133619169 | + | FSTL4 | NNNAGGTACACAGGAAGAGG | NGG | 2 | 0.0 | Tier II |
35 | NC_000001.11 | 1 | 24089259 | + | MYOM3 | NNNAGGTACCCAGGAAGTGG | NGG | 2 | 0.0 | Tier II |
36 | NC_000006.12 | 6 | 118684084 | + | CEP85L | NNNAGTTACACAGGAAGTGG | NGG | 2 | 0.0 | Tier II |
37 | NC_000015.10 | 15 | 60542797 | - | RORA-AS1 | NNNAGGTTCTCAGCAAGTGG | NGG | 2 | 0.3782 | Tier III |
38 | NC_000015.10 | 15 | 60543050 | - | RORA-AS1 | NNNAGGTTCTCAGCAAGTGG | NGG | 2 | 0.3782 | Tier III |
39 | NC_000005.10 | 5 | 1720439 | - | LOC105374614 | NNNAGGCTCACAGCAAGTGG | NGG | 2 | 0.2946 | Tier III |
40 | NC_000001.11 | 1 | 174365644 | - | LOC100302291 | NNNAGGAACACAGCAACTGG | NGG | 2 | 0.2059 | Tier III |
41 | NC_000001.11 | 1 | 212629236 | + | LINC02773 | NNNAGGTACAGAGCCAGTGG | NGG | 2 | 0.0682 | Tier III |
42 | NC_000017.11 | 17 | 37799982 | + | LOC105371757 | NNNAGGTGCACAGGAAGTGG | NGG | 2 | 0.0 | Tier III |
43 | NC_000006.12 | 6 | 118684084 | + | LOC124901388 | NNNAGTTACACAGGAAGTGG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000077.6 | 11 | 51254021 | + | BC049762 | NNNGGGTACACAGAAAGTGG | NGG | 2 | 0.2188 | Tier I |
2 | NC_000068.7 | 2 | 25353237 | + | Dpp7 | NNNAGGAACACAGCAAGTGG | NGG | 1 | 0.875 | Tier II |
3 | NC_000067.6 | 1 | 188539568 | + | Ush2a | NNNAAGTACACAGCAAGTGA | NGG | 2 | 0.8125 | Tier II |
4 | NC_000081.6 | 15 | 78617336 | - | Cyth4 | NNNAGGTACACAGTAAGTGA | NGG | 2 | 0.6875 | Tier II |
5 | NC_000068.7 | 2 | 62277735 | + | Slc4a10 | NNNAGGTACTCAGCAAGTAG | NGG | 2 | 0.6303 | Tier II |
6 | NC_000081.6 | 15 | 89148342 | - | Mapk11 | NNNAGGGACTCAGCAAGTGG | NGG | 2 | 0.519 | Tier II |
7 | NC_000076.6 | 10 | 41461010 | + | Zbtb24 | NNNAGGGAAACAGCAAGTGG | NGG | 2 | 0.5042 | Tier II |
8 | NC_000068.7 | 2 | 24955038 | - | Zmynd19 | NNNAGCTACACAGCAAGTTG | NGG | 2 | 0.4545 | Tier II |
9 | NC_000068.7 | 2 | 144532591 | + | Polr3f | NNNAGCTACACAGCAAGTTG | NGG | 2 | 0.4545 | Tier II |
10 | NC_000077.6 | 11 | 88200944 | - | Mrps23 | NNNAGGTACACAGTAAGTGC | NGG | 2 | 0.3143 | Tier II |
11 | NC_000068.7 | 2 | 144738487 | + | Dtd1 | NNNAGGTACACAGCAAGGGA | NGG | 2 | 0.3125 | Tier II |
12 | NC_000069.6 | 3 | 148886087 | + | Adgrl2 | NNNAGGTACATAGCAAGTAG | NGG | 2 | 0.2198 | Tier II |
13 | NC_000069.6 | 3 | 104060395 | - | Magi3 | NNNAGGTGCACAGCAAGTGG | NAG | 2 | 0.1901 | Tier II |
14 | NC_000067.6 | 1 | 175679802 | + | Opn3 | NNNAGCTACACAGCAAGTGG | NAG | 2 | 0.1768 | Tier II |
15 | NC_000079.6 | 13 | 109378998 | - | Pde4d | NNNAGGTACTCAGCTAGTGG | NGG | 2 | 0.1765 | Tier II |
16 | NC_000070.6 | 4 | 36177500 | - | Lingo2 | NNNTGGTACACAGCAAGTGG | NAG | 2 | 0.165 | Tier II |
17 | NC_000080.6 | 14 | 56533936 | - | Cenpj | NNNAGGTACACAGCAAGGGC | NGG | 2 | 0.1429 | Tier II |
18 | NC_000068.7 | 2 | 120125139 | - | Ehd4 | NNNAGGGACACAGCAACTGG | NGG | 2 | 0.1384 | Tier II |
19 | NC_000085.6 | 19 | 46451829 | - | Sufu | NNNAGGTACCCAGAAAGTGG | NGG | 2 | 0.1167 | Tier II |
20 | NC_000086.7 | X | 161950925 | - | Nhs | NNNAGGTACACAGCAAGTCG | NGA | 2 | 0.0311 | Tier II |
21 | NC_000073.6 | 7 | 81538203 | + | Fsd2 | NNNAGGTAAACAGCAAGTGG | NGC | 2 | 0.019 | Tier II |
22 | NC_000072.6 | 6 | 17537513 | - | Met | NNNAGGGACACAGCAAGTGG | NGC | 2 | 0.0131 | Tier II |
23 | NC_000075.6 | 9 | 22134452 | - | Acp5 | NNNAGGCACACAGGAAGTGG | NGG | 2 | 0.0 | Tier II |
24 | NC_000080.6 | 14 | 70406490 | - | Piwil2 | NNNAGGGACACAGCATGTGG | NGG | 2 | 0.0 | Tier II |
25 | NC_000072.6 | 6 | 38868245 | + | Hipk2 | NNNAGGTACACAGAATGTGG | NGG | 2 | 0.0 | Tier II |
26 | NC_000072.6 | 6 | 38868245 | + | Tbxas1 | NNNAGGTACACAGAATGTGG | NGG | 2 | 0.0 | Tier II |
27 | NC_000068.7 | 2 | 122242195 | + | Sord | NNNAGGTACACAGCACGTTG | NGG | 2 | 0.0 | Tier II |
28 | NC_000067.6 | 1 | 124660039 | - | Dpp10 | NNNAGGTACACAGCATGTGG | NGT | 2 | 0.0 | Tier II |
29 | NC_000070.6 | 4 | 126234755 | - | Map7d1 | NNNAGGTACACAGGAAGTGA | NGG | 2 | 0.0 | Tier II |
30 | NC_000075.6 | 9 | 23130775 | + | Gm36743 | NNNAAGTACACAGCAAATGG | NGG | 2 | 0.8089 | Tier III |
31 | NC_000069.6 | 3 | 97331219 | + | Gm40101 | NNNAGGTACTCAGCAAGTGC | NGG | 2 | 0.3782 | Tier III |
32 | NC_000073.6 | 7 | 84178726 | - | LOC117997403 | NNNAGCTACACAGCAAGTCG | NGG | 2 | 0.3056 | Tier III |
33 | NC_000069.6 | 3 | 107763123 | - | Gm40121 | NNNAGTTACACAGCAAGTGG | NTG | 2 | 0.026 | Tier III |
34 | NC_000081.6 | 15 | 24993663 | - | Gm2824 | NNNAGGTAAACAGCAAGTGG | NGC | 2 | 0.019 | Tier III |
35 | NC_000072.6 | 6 | 17537513 | - | Gm52893 | NNNAGGGACACAGCAAGTGG | NGC | 2 | 0.0131 | Tier III |
36 | NC_000078.6 | 12 | 113421245 | + | Ighm | NNNAGGTACACAGCAGGTGG | NTG | 2 | 0.0075 | Tier III |
37 | NC_000078.6 | 12 | 113421245 | + | Igh | NNNAGGTACACAGCAGGTGG | NTG | 2 | 0.0075 | Tier III |
38 | NC_000068.7 | 2 | 75183542 | - | 2600014E21Rik | NNNAGGTAGACAGGAAGTGG | NGG | 2 | 0.0 | Tier III |