Construct: sgRNA BRDN0001149039

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAAGGTACACAGCAAGTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD3 (8019)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76093

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 134050577 - BRD3 NNNAGGTACACAGCAAGTGG NGG 0 1.0 Tier I
2 NC_000001.11 1 33427797 + PHC2 NNNAGGTACACAACAAATGG NGG 2 0.8615 Tier II
3 NC_000006.12 6 162097465 + PRKN NNNACATACACAGCAAGTGG NGG 2 0.7857 Tier II
4 NC_000010.11 10 21798599 - DNAJC1 NNNAGGTACAAAGCAAGTGA NGG 2 0.7031 Tier II
5 NC_000011.10 11 68034324 - NDUFS8 NNNAGCTACACAGCAAGTGG NGG 1 0.6818 Tier II
6 NC_000007.14 7 140060706 - PARP12 NNNAGGCACACAGCAAATGG NGG 2 0.6417 Tier II
7 NC_000004.12 4 138200040 - SLC7A11 NNNAGATAGACAGCAAGTGG NGG 2 0.619 Tier II
8 NC_000010.11 10 80465260 - TSPAN14 NNNAGGCACAAAGCAAGTGG NGG 2 0.5156 Tier II
9 NC_000022.11 22 37810170 + GCAT NNNAGGTACACAGTGAGTGG NGG 2 0.4767 Tier II
10 NC_000003.12 3 14690483 + C3orf20 NNNAGTCACACAGCAAGTGG NGG 2 0.4583 Tier II
11 NC_000009.12 9 288388 - DOCK8 NNNAGTCACACAGCAAGTGG NGG 2 0.4583 Tier II
12 NC_000015.10 15 60542797 - RORA NNNAGGTTCTCAGCAAGTGG NGG 2 0.3782 Tier II
13 NC_000015.10 15 60543050 - RORA NNNAGGTTCTCAGCAAGTGG NGG 2 0.3782 Tier II
14 NC_000017.11 17 45128263 + PLCD3 NNNTGGGACACAGCAAGTGG NGG 2 0.3743 Tier II
15 NC_000017.11 17 80718084 - RPTOR NNNAGGTACACAGCAAAGGG NGG 2 0.3111 Tier II
16 NC_000021.9 21 45802309 - PCBP3 NNNAGGAACACAGCAAGGGG NGG 2 0.2917 Tier II
17 NC_000010.11 10 75094570 - DUSP13B NNNATGTAAACAGCAAGTGG NGG 2 0.2571 Tier II
18 NC_000001.11 1 33812228 + CSMD2 NNNAGGGCCACAGCAAGTGG NGG 2 0.2521 Tier II
19 NC_000018.10 18 8130818 + PTPRM NNNATGTACACAGCAAGTAG NGG 2 0.2143 Tier II
20 NC_000001.11 1 174365644 - RABGAP1L NNNAGGAACACAGCAACTGG NGG 2 0.2059 Tier II
21 NC_000003.12 3 54298261 + CACNA2D3 NNNAGGTACAAAGCCAGTGG NGG 2 0.2045 Tier II
22 NC_000005.10 5 64583920 - RGS7BP NNNAGGTACACAGTAAGTGG NAG 2 0.1901 Tier II
23 NC_000001.11 1 7927529 + TNFRSF9 NNNAGGTCCACAGCAAGTGC NGG 2 0.1837 Tier II
24 NC_000004.12 4 169164511 + SH3RF1 NNNAAGTACACAGCAGGTGG NGG 2 0.1667 Tier II
25 NC_000006.12 6 42103670 - C6orf132 NNNAGGTACACAGCAGGTGT NGG 2 0.1346 Tier II
26 NC_000011.10 11 113425399 + DRD2 NNNAGGTAGACAGCAGGTGG NGG 2 0.119 Tier II
27 NC_000004.12 4 152805833 + ARFIP1 NNNAGGTTCACAGCTAGTGG NGG 2 0.0857 Tier II
28 NC_000020.11 20 62152690 + SS18L1 NNNAGGTACACACCAGGTGG NGG 2 0.081 Tier II
29 NC_000016.10 16 14662466 - BFAR NNNAGGTAAACAGCAAGTGG NGA 2 0.0595 Tier II
30 NC_000013.11 13 51401761 + INTS6 NNNAGGTACACAGTAAGTGG NGA 2 0.0509 Tier II
31 NC_000009.12 9 120454134 + CDK5RAP2 NNNAGGTACACAGCAAGTGA NTG 2 0.0365 Tier II
32 NC_000019.10 19 54906112 - NCR1 NNNAGATACACAGGAAGTGG NGG 2 0.0 Tier II
33 NC_000016.10 16 73010065 + ZFHX3 NNNAGGTACACAGCACCTGG NGG 2 0.0 Tier II
34 NC_000005.10 5 133619169 + FSTL4 NNNAGGTACACAGGAAGAGG NGG 2 0.0 Tier II
35 NC_000001.11 1 24089259 + MYOM3 NNNAGGTACCCAGGAAGTGG NGG 2 0.0 Tier II
36 NC_000006.12 6 118684084 + CEP85L NNNAGTTACACAGGAAGTGG NGG 2 0.0 Tier II
37 NC_000015.10 15 60542797 - RORA-AS1 NNNAGGTTCTCAGCAAGTGG NGG 2 0.3782 Tier III
38 NC_000015.10 15 60543050 - RORA-AS1 NNNAGGTTCTCAGCAAGTGG NGG 2 0.3782 Tier III
39 NC_000005.10 5 1720439 - LOC105374614 NNNAGGCTCACAGCAAGTGG NGG 2 0.2946 Tier III
40 NC_000001.11 1 174365644 - LOC100302291 NNNAGGAACACAGCAACTGG NGG 2 0.2059 Tier III
41 NC_000001.11 1 212629236 + LINC02773 NNNAGGTACAGAGCCAGTGG NGG 2 0.0682 Tier III
42 NC_000017.11 17 37799982 + LOC105371757 NNNAGGTGCACAGGAAGTGG NGG 2 0.0 Tier III
43 NC_000006.12 6 118684084 + LOC124901388 NNNAGTTACACAGGAAGTGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 51254021 + BC049762 NNNGGGTACACAGAAAGTGG NGG 2 0.2188 Tier I
2 NC_000068.7 2 25353237 + Dpp7 NNNAGGAACACAGCAAGTGG NGG 1 0.875 Tier II
3 NC_000067.6 1 188539568 + Ush2a NNNAAGTACACAGCAAGTGA NGG 2 0.8125 Tier II
4 NC_000081.6 15 78617336 - Cyth4 NNNAGGTACACAGTAAGTGA NGG 2 0.6875 Tier II
5 NC_000068.7 2 62277735 + Slc4a10 NNNAGGTACTCAGCAAGTAG NGG 2 0.6303 Tier II
6 NC_000081.6 15 89148342 - Mapk11 NNNAGGGACTCAGCAAGTGG NGG 2 0.519 Tier II
7 NC_000076.6 10 41461010 + Zbtb24 NNNAGGGAAACAGCAAGTGG NGG 2 0.5042 Tier II
8 NC_000068.7 2 24955038 - Zmynd19 NNNAGCTACACAGCAAGTTG NGG 2 0.4545 Tier II
9 NC_000068.7 2 144532591 + Polr3f NNNAGCTACACAGCAAGTTG NGG 2 0.4545 Tier II
10 NC_000077.6 11 88200944 - Mrps23 NNNAGGTACACAGTAAGTGC NGG 2 0.3143 Tier II
11 NC_000068.7 2 144738487 + Dtd1 NNNAGGTACACAGCAAGGGA NGG 2 0.3125 Tier II
12 NC_000069.6 3 148886087 + Adgrl2 NNNAGGTACATAGCAAGTAG NGG 2 0.2198 Tier II
13 NC_000069.6 3 104060395 - Magi3 NNNAGGTGCACAGCAAGTGG NAG 2 0.1901 Tier II
14 NC_000067.6 1 175679802 + Opn3 NNNAGCTACACAGCAAGTGG NAG 2 0.1768 Tier II
15 NC_000079.6 13 109378998 - Pde4d NNNAGGTACTCAGCTAGTGG NGG 2 0.1765 Tier II
16 NC_000070.6 4 36177500 - Lingo2 NNNTGGTACACAGCAAGTGG NAG 2 0.165 Tier II
17 NC_000080.6 14 56533936 - Cenpj NNNAGGTACACAGCAAGGGC NGG 2 0.1429 Tier II
18 NC_000068.7 2 120125139 - Ehd4 NNNAGGGACACAGCAACTGG NGG 2 0.1384 Tier II
19 NC_000085.6 19 46451829 - Sufu NNNAGGTACCCAGAAAGTGG NGG 2 0.1167 Tier II
20 NC_000086.7 X 161950925 - Nhs NNNAGGTACACAGCAAGTCG NGA 2 0.0311 Tier II
21 NC_000073.6 7 81538203 + Fsd2 NNNAGGTAAACAGCAAGTGG NGC 2 0.019 Tier II
22 NC_000072.6 6 17537513 - Met NNNAGGGACACAGCAAGTGG NGC 2 0.0131 Tier II
23 NC_000075.6 9 22134452 - Acp5 NNNAGGCACACAGGAAGTGG NGG 2 0.0 Tier II
24 NC_000080.6 14 70406490 - Piwil2 NNNAGGGACACAGCATGTGG NGG 2 0.0 Tier II
25 NC_000072.6 6 38868245 + Hipk2 NNNAGGTACACAGAATGTGG NGG 2 0.0 Tier II
26 NC_000072.6 6 38868245 + Tbxas1 NNNAGGTACACAGAATGTGG NGG 2 0.0 Tier II
27 NC_000068.7 2 122242195 + Sord NNNAGGTACACAGCACGTTG NGG 2 0.0 Tier II
28 NC_000067.6 1 124660039 - Dpp10 NNNAGGTACACAGCATGTGG NGT 2 0.0 Tier II
29 NC_000070.6 4 126234755 - Map7d1 NNNAGGTACACAGGAAGTGA NGG 2 0.0 Tier II
30 NC_000075.6 9 23130775 + Gm36743 NNNAAGTACACAGCAAATGG NGG 2 0.8089 Tier III
31 NC_000069.6 3 97331219 + Gm40101 NNNAGGTACTCAGCAAGTGC NGG 2 0.3782 Tier III
32 NC_000073.6 7 84178726 - LOC117997403 NNNAGCTACACAGCAAGTCG NGG 2 0.3056 Tier III
33 NC_000069.6 3 107763123 - Gm40121 NNNAGTTACACAGCAAGTGG NTG 2 0.026 Tier III
34 NC_000081.6 15 24993663 - Gm2824 NNNAGGTAAACAGCAAGTGG NGC 2 0.019 Tier III
35 NC_000072.6 6 17537513 - Gm52893 NNNAGGGACACAGCAAGTGG NGC 2 0.0131 Tier III
36 NC_000078.6 12 113421245 + Ighm NNNAGGTACACAGCAGGTGG NTG 2 0.0075 Tier III
37 NC_000078.6 12 113421245 + Igh NNNAGGTACACAGCAGGTGG NTG 2 0.0075 Tier III
38 NC_000068.7 2 75183542 - 2600014E21Rik NNNAGGTAGACAGGAAGTGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)