Construct: sgRNA BRDN0001149042
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGAAACACCAATTTCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK1A (1859)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76756
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
37490273 |
+ |
DYRK1A |
NNNGAAACACCAATTTCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
168682372 |
- |
SLIT3 |
NNNGAAACACCAGTTTCCAA |
NGG |
2 |
0.4658 |
Tier II |
| 3 |
NC_000009.12 |
9 |
78242833 |
+ |
CEP78 |
NNNAAACCACCAATTTCCGA |
NGG |
2 |
0.3938 |
Tier II |
| 4 |
NC_000011.10 |
11 |
11620043 |
+ |
GALNT18 |
NNNGAAACACCAAATTCAGA |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000004.12 |
4 |
30818674 |
+ |
PCDH7 |
NNNGGAACACCAATTTCCCA |
NGG |
2 |
0.3228 |
Tier II |
| 6 |
NC_000022.11 |
22 |
23072477 |
- |
GNAZ |
NNNGTGACACCAATTTCCGA |
NGG |
2 |
0.2597 |
Tier II |
| 7 |
NC_000022.11 |
22 |
23072477 |
- |
RSPH14 |
NNNGTGACACCAATTTCCGA |
NGG |
2 |
0.2597 |
Tier II |
| 8 |
NC_000001.11 |
1 |
58099040 |
- |
DAB1 |
NNNGAAACACCCATTTCTGA |
NGG |
2 |
0.1692 |
Tier II |
| 9 |
NC_000016.10 |
16 |
72366289 |
- |
LINC01572 |
NNNGAAACACCAATATCAGA |
NGG |
2 |
0.3117 |
Tier III |
| 10 |
NC_000015.10 |
15 |
100863938 |
+ |
GCAWKR |
NNNTAAACACCAGTTTCCGA |
NGG |
2 |
0.2372 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
94671401 |
+ |
Dyrk1a |
NNNGAAACACCAACTTCCGA |
NGG |
1 |
0.2857 |
Tier I |
| 2 |
NC_000080.6 |
14 |
121906729 |
- |
Ubac2 |
NNNTAAACACCAATTTCCAA |
NGG |
2 |
0.2597 |
Tier II |
| 3 |
NC_000068.7 |
2 |
42105377 |
- |
Lrp1b |
NNNGAAACACCAATTTCCAA |
NGC |
2 |
0.0159 |
Tier II |
| 4 |
NC_000070.6 |
4 |
113782452 |
+ |
Skint5 |
NNNGAAACATCAAGTTCCGA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
