Construct: sgRNA BRDN0001149045
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATGTCATCTACATACTGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NOL9 (79707)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76980
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
6532656 |
+ |
NOL9 |
NNNGTCATCTACATACTGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
3723408 |
- |
TP73 |
NNNGTCATCCACATACTGCG |
NGA |
2 |
0.037 |
Tier I |
| 3 |
NC_000019.10 |
19 |
34622710 |
+ |
SCGB2B2 |
NNNGTCATCTACATACTGCA |
NAG |
2 |
0.2431 |
Tier II |
| 4 |
NC_000004.12 |
4 |
93394522 |
- |
GRID2 |
NNNGTCATCTACATACTGGC |
NGG |
2 |
0.0536 |
Tier II |
| 5 |
NC_000019.10 |
19 |
34622710 |
+ |
SCGB1B2P |
NNNGTCATCTACATACTGCA |
NAG |
2 |
0.2431 |
Tier III |
| 6 |
NC_000019.10 |
19 |
34622710 |
+ |
ZNF807P |
NNNGTCATCTACATACTGCA |
NAG |
2 |
0.2431 |
Tier III |
| 7 |
NC_000023.11 |
X |
129164835 |
+ |
LOC124905213 |
NNNGTCATCTACATACTTGG |
NGG |
2 |
0.0833 |
Tier III |
| 8 |
NC_000002.12 |
2 |
41497674 |
- |
LOC105374506 |
NNNGTCATCTACATACTGCA |
NGA |
2 |
0.0651 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
36769089 |
- |
Dlc1 |
NNNGTCATCTATATACTGCT |
NGG |
2 |
0.3769 |
Tier II |
| 2 |
NC_000073.6 |
7 |
82577636 |
+ |
Adamtsl3 |
NNNGTCAGCTACATACTGTG |
NGG |
2 |
0.3385 |
Tier II |
| 3 |
NC_000079.6 |
13 |
8433021 |
+ |
Adarb2 |
NNNGTCATCTACATTCTGGG |
NGG |
2 |
0.025 |
Tier II |
Other clones with same target sequence:
(none)
