Construct: sgRNA BRDN0001149049

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTCAGTGGAAAGCGACTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNK2 (10188)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75584

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 195885015 - TNK2 NNNCAGTGGAAAGCGACTGG NGG 0 1.0 Tier I
2 NC_000001.11 1 14805401 - KAZN NNNCAATGGAAAACGACTGG NGG 2 0.9231 Tier II
3 NC_000005.10 5 134548170 + JADE2 NNNCAGTGGAGAGCGACTGG NGG 1 0.65 Tier II
4 NC_000012.12 12 20475941 + PDE3A NNNCAGAGGAAAGTGACTGG NGG 2 0.6417 Tier II
5 NC_000014.9 14 80634922 - CEP128 NNNCAGTGGGAAGCAACTGG NGG 2 0.5229 Tier II
6 NC_000015.10 15 51307116 + CYP19A1 NNNCAATGGAAAGCCACTGG NGG 2 0.2727 Tier II
7 NC_000016.10 16 84898293 - CRISPLD2 NNNCAATGGAAAGCCACTGG NGG 2 0.2727 Tier II
8 NC_000008.11 8 98521587 - STK3 NNNCAGTGGAAAGCCACTGA NGG 2 0.2557 Tier II
9 NC_000010.11 10 13134263 - OPTN NNNCACTGGAAAGAGACTGG NGG 2 0.2386 Tier II
10 NC_000011.10 11 121537365 + SORL1 NNNCAGTGGAGAGAGACTGG NGG 2 0.2275 Tier II
11 NC_000001.11 1 210953701 - KCNH1 NNNCACTGGAAAGCCACTGG NGG 2 0.186 Tier II
12 NC_000004.12 4 147844926 - ARHGAP10 NNNCAGTGGAAAGCCACAGG NGG 2 0.1818 Tier II
13 NC_000001.11 1 116631379 + IGSF3 NNNCAGTGGAGAGCCACTGG NGG 2 0.1773 Tier II
14 NC_000005.10 5 64534379 + RGS7BP NNNCAGTGAAAAGCCACTGG NGG 2 0.1753 Tier II
15 NC_000003.12 3 170518536 - SLC7A14 NNNCAGTGGAAAGAGAATGG NGG 2 0.1633 Tier II
16 NC_000009.12 9 38041690 + SHB NNNCAGTGGGAAGCCACTGG NGG 2 0.1515 Tier II
17 NC_000009.12 9 98150254 - CORO2A NNNCAGTGGGAAGCCACTGG NGG 2 0.1515 Tier II
18 NC_000017.11 17 27878635 + LYRM9 NNNCAGTGGAAAGAGACTGC NGG 2 0.15 Tier II
19 NC_000014.9 14 24327348 + ADCY4 NNNCAGTGGAAAGCGGCAGG NGG 2 0.1282 Tier II
20 NC_000018.10 18 46581048 + LOXHD1 NNNCAGTGGAAAGCCATTGG NGG 2 0.1273 Tier II
21 NC_000020.11 20 14094864 - MACROD2 NNNCAGTGGCAAGAGACTGG NGG 2 0.1167 Tier II
22 NC_000012.12 12 5708350 - ANO2 NNNCAGTGGAAAGTTACTGG NGG 2 0.1048 Tier II
23 NC_000018.10 18 7751277 - PTPRM NNNCAGTGGAAAGCGACTGG NGA 1 0.0694 Tier II
24 NC_000002.12 2 44621754 + CAMKMT NNNCAGTGGAAAGCTATTGG NGG 2 0.0667 Tier II
25 NC_000008.11 8 3600635 + CSMD1 NNNCAGTGGAAAACGACTGG NGA 2 0.0641 Tier II
26 NC_000006.12 6 55487802 - HMGCLL1 NNNCAGTGGACAGCTACTGG NGG 2 0.0571 Tier II
27 NC_000007.14 7 75993877 - TMEM120A NNNCAGTGGAAAGCTACGGG NGG 2 0.0476 Tier II
28 NC_000012.12 12 44172133 + TMEM117 NNNCAGTGGAAAGCCACTGG NGA 2 0.0189 Tier II
29 NC_000006.12 6 63332643 - LGSN NNNCAGTGGAAAGCGACTAG NGC 2 0.0159 Tier II
30 NC_000015.10 15 51307116 + LOC124903493 NNNCAATGGAAAGCCACTGG NGG 2 0.2727 Tier III
31 NC_000006.12 6 112266241 + LAMA4-AS1 NNNCAGTGGAGAGAGACTGG NGG 2 0.2275 Tier III
32 NC_000002.12 2 47313144 - EPCAM-DT NNNCAGTGGAAAGCCACAGG NGG 2 0.1818 Tier III
33 NC_000022.11 22 21243917 + GGT2P NNNCAGTGGAGAGCCACTGG NGG 2 0.1773 Tier III
34 NC_000003.12 3 170518536 - SLC7A14-AS1 NNNCAGTGGAAAGAGAATGG NGG 2 0.1633 Tier III
35 NC_000009.12 9 127587995 - LOC124902277 NNNCAGTGGAAAGCCATTGG NGG 2 0.1273 Tier III
36 NC_000012.12 12 52232887 - TMT1AP1 NNNCAGTGGCAAGAGACTGG NGG 2 0.1167 Tier III
37 NC_000003.12 3 635600 + LINC01266 NNNCAGTGGAAAGAGACTGG NGA 2 0.0243 Tier III
38 NC_000017.11 17 49566345 + NGFR-AS1 NNNAAGTGGAAAGGGACTGG NGG 2 0.0 Tier III
39 NC_000017.11 17 72408136 - LINC00673 NNNAAGTGGAAAGGGACTGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 32668240 + Tnk2 NNNCAGTGGAAAGCGGTTGG NGG 2 0.0897 Tier I
2 NC_000071.6 5 63807750 + Nwd2 NNNCAGTGGAAAGCTACGGG NGG 2 0.0476 Tier I
3 NC_000071.6 5 149204529 - Uspl1 NNNCAGTGGTAAGCAACTGG NGG 2 0.8304 Tier II
4 NC_000085.6 19 42776784 + Hps1 NNNAACTGGAAAGCGACTGG NGG 2 0.5742 Tier II
5 NC_000076.6 10 60113347 + Spock2 NNNCAGTGGAAAGCGACAGT NGG 2 0.4667 Tier II
6 NC_000075.6 9 45851905 + Bace1 NNNCAGTGGAAAGTGACTGC NGG 2 0.3143 Tier II
7 NC_000070.6 4 113128966 + Skint6 NNNTAGTGGAAAGCGACTGG NAG 2 0.2074 Tier II
8 NC_000072.6 6 30563336 + Cpa2 NNNCAGTGGAAAGCCACAGG NGG 2 0.1818 Tier II
9 NC_000067.6 1 58859887 + Gm28802 NNNGAGTGGAAAGCGACTGG NAG 2 0.1296 Tier II
10 NC_000069.6 3 105981570 - Ovgp1 NNNGAGTGGAAAGCGACTGG NAG 2 0.1296 Tier II
11 NC_000070.6 4 102774910 + Sgip1 NNNCAGTGGAAAGCCACTGG NAG 2 0.0707 Tier II
12 NC_000075.6 9 119536354 + Scn5a NNNCAGTGGAAAGCTACTGG NGA 2 0.0099 Tier II
13 NC_000079.6 13 24504555 - Ripor2 NNNCAGTGGAAAGCGCCTTG NGG 2 0.0 Tier II
14 NC_000067.6 1 46544593 - Dnah7c NNNCAGTGGAAAGCGTCTGG NAG 2 0.0 Tier II
15 NC_000084.6 18 35847933 - Cxxc5 NNNCAGTGGAGAGGGACTGG NGG 2 0.0 Tier II
16 NC_000076.6 10 60113347 + Gm51801 NNNCAGTGGAAAGCGACAGT NGG 2 0.4667 Tier III
17 NC_000068.7 2 173648334 - Ppp4r1l-ps NNNCAGTGGACAGAGACTGG NGG 2 0.14 Tier III
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Other clones with same target sequence:

(none)