Construct: sgRNA BRDN0001149049
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTCAGTGGAAAGCGACTGG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- TNK2 (10188)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000003.12 | 3 | 195885015 | - | TNK2 | NNNCAGTGGAAAGCGACTGG | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 14805401 | - | KAZN | NNNCAATGGAAAACGACTGG | NGG | 2 | 0.9231 | Tier II |
3 | NC_000005.10 | 5 | 134548170 | + | JADE2 | NNNCAGTGGAGAGCGACTGG | NGG | 1 | 0.65 | Tier II |
4 | NC_000012.12 | 12 | 20475941 | + | PDE3A | NNNCAGAGGAAAGTGACTGG | NGG | 2 | 0.6417 | Tier II |
5 | NC_000014.9 | 14 | 80634922 | - | CEP128 | NNNCAGTGGGAAGCAACTGG | NGG | 2 | 0.5229 | Tier II |
6 | NC_000015.10 | 15 | 51307116 | + | CYP19A1 | NNNCAATGGAAAGCCACTGG | NGG | 2 | 0.2727 | Tier II |
7 | NC_000016.10 | 16 | 84898293 | - | CRISPLD2 | NNNCAATGGAAAGCCACTGG | NGG | 2 | 0.2727 | Tier II |
8 | NC_000008.11 | 8 | 98521587 | - | STK3 | NNNCAGTGGAAAGCCACTGA | NGG | 2 | 0.2557 | Tier II |
9 | NC_000010.11 | 10 | 13134263 | - | OPTN | NNNCACTGGAAAGAGACTGG | NGG | 2 | 0.2386 | Tier II |
10 | NC_000011.10 | 11 | 121537365 | + | SORL1 | NNNCAGTGGAGAGAGACTGG | NGG | 2 | 0.2275 | Tier II |
11 | NC_000001.11 | 1 | 210953701 | - | KCNH1 | NNNCACTGGAAAGCCACTGG | NGG | 2 | 0.186 | Tier II |
12 | NC_000004.12 | 4 | 147844926 | - | ARHGAP10 | NNNCAGTGGAAAGCCACAGG | NGG | 2 | 0.1818 | Tier II |
13 | NC_000001.11 | 1 | 116631379 | + | IGSF3 | NNNCAGTGGAGAGCCACTGG | NGG | 2 | 0.1773 | Tier II |
14 | NC_000005.10 | 5 | 64534379 | + | RGS7BP | NNNCAGTGAAAAGCCACTGG | NGG | 2 | 0.1753 | Tier II |
15 | NC_000003.12 | 3 | 170518536 | - | SLC7A14 | NNNCAGTGGAAAGAGAATGG | NGG | 2 | 0.1633 | Tier II |
16 | NC_000009.12 | 9 | 38041690 | + | SHB | NNNCAGTGGGAAGCCACTGG | NGG | 2 | 0.1515 | Tier II |
17 | NC_000009.12 | 9 | 98150254 | - | CORO2A | NNNCAGTGGGAAGCCACTGG | NGG | 2 | 0.1515 | Tier II |
18 | NC_000017.11 | 17 | 27878635 | + | LYRM9 | NNNCAGTGGAAAGAGACTGC | NGG | 2 | 0.15 | Tier II |
19 | NC_000014.9 | 14 | 24327348 | + | ADCY4 | NNNCAGTGGAAAGCGGCAGG | NGG | 2 | 0.1282 | Tier II |
20 | NC_000018.10 | 18 | 46581048 | + | LOXHD1 | NNNCAGTGGAAAGCCATTGG | NGG | 2 | 0.1273 | Tier II |
21 | NC_000020.11 | 20 | 14094864 | - | MACROD2 | NNNCAGTGGCAAGAGACTGG | NGG | 2 | 0.1167 | Tier II |
22 | NC_000012.12 | 12 | 5708350 | - | ANO2 | NNNCAGTGGAAAGTTACTGG | NGG | 2 | 0.1048 | Tier II |
23 | NC_000018.10 | 18 | 7751277 | - | PTPRM | NNNCAGTGGAAAGCGACTGG | NGA | 1 | 0.0694 | Tier II |
24 | NC_000002.12 | 2 | 44621754 | + | CAMKMT | NNNCAGTGGAAAGCTATTGG | NGG | 2 | 0.0667 | Tier II |
25 | NC_000008.11 | 8 | 3600635 | + | CSMD1 | NNNCAGTGGAAAACGACTGG | NGA | 2 | 0.0641 | Tier II |
26 | NC_000006.12 | 6 | 55487802 | - | HMGCLL1 | NNNCAGTGGACAGCTACTGG | NGG | 2 | 0.0571 | Tier II |
27 | NC_000007.14 | 7 | 75993877 | - | TMEM120A | NNNCAGTGGAAAGCTACGGG | NGG | 2 | 0.0476 | Tier II |
28 | NC_000012.12 | 12 | 44172133 | + | TMEM117 | NNNCAGTGGAAAGCCACTGG | NGA | 2 | 0.0189 | Tier II |
29 | NC_000006.12 | 6 | 63332643 | - | LGSN | NNNCAGTGGAAAGCGACTAG | NGC | 2 | 0.0159 | Tier II |
30 | NC_000015.10 | 15 | 51307116 | + | LOC124903493 | NNNCAATGGAAAGCCACTGG | NGG | 2 | 0.2727 | Tier III |
31 | NC_000006.12 | 6 | 112266241 | + | LAMA4-AS1 | NNNCAGTGGAGAGAGACTGG | NGG | 2 | 0.2275 | Tier III |
32 | NC_000002.12 | 2 | 47313144 | - | EPCAM-DT | NNNCAGTGGAAAGCCACAGG | NGG | 2 | 0.1818 | Tier III |
33 | NC_000022.11 | 22 | 21243917 | + | GGT2P | NNNCAGTGGAGAGCCACTGG | NGG | 2 | 0.1773 | Tier III |
34 | NC_000003.12 | 3 | 170518536 | - | SLC7A14-AS1 | NNNCAGTGGAAAGAGAATGG | NGG | 2 | 0.1633 | Tier III |
35 | NC_000009.12 | 9 | 127587995 | - | LOC124902277 | NNNCAGTGGAAAGCCATTGG | NGG | 2 | 0.1273 | Tier III |
36 | NC_000012.12 | 12 | 52232887 | - | TMT1AP1 | NNNCAGTGGCAAGAGACTGG | NGG | 2 | 0.1167 | Tier III |
37 | NC_000003.12 | 3 | 635600 | + | LINC01266 | NNNCAGTGGAAAGAGACTGG | NGA | 2 | 0.0243 | Tier III |
38 | NC_000017.11 | 17 | 49566345 | + | NGFR-AS1 | NNNAAGTGGAAAGGGACTGG | NGG | 2 | 0.0 | Tier III |
39 | NC_000017.11 | 17 | 72408136 | - | LINC00673 | NNNAAGTGGAAAGGGACTGG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000082.6 | 16 | 32668240 | + | Tnk2 | NNNCAGTGGAAAGCGGTTGG | NGG | 2 | 0.0897 | Tier I |
2 | NC_000071.6 | 5 | 63807750 | + | Nwd2 | NNNCAGTGGAAAGCTACGGG | NGG | 2 | 0.0476 | Tier I |
3 | NC_000071.6 | 5 | 149204529 | - | Uspl1 | NNNCAGTGGTAAGCAACTGG | NGG | 2 | 0.8304 | Tier II |
4 | NC_000085.6 | 19 | 42776784 | + | Hps1 | NNNAACTGGAAAGCGACTGG | NGG | 2 | 0.5742 | Tier II |
5 | NC_000076.6 | 10 | 60113347 | + | Spock2 | NNNCAGTGGAAAGCGACAGT | NGG | 2 | 0.4667 | Tier II |
6 | NC_000075.6 | 9 | 45851905 | + | Bace1 | NNNCAGTGGAAAGTGACTGC | NGG | 2 | 0.3143 | Tier II |
7 | NC_000070.6 | 4 | 113128966 | + | Skint6 | NNNTAGTGGAAAGCGACTGG | NAG | 2 | 0.2074 | Tier II |
8 | NC_000072.6 | 6 | 30563336 | + | Cpa2 | NNNCAGTGGAAAGCCACAGG | NGG | 2 | 0.1818 | Tier II |
9 | NC_000067.6 | 1 | 58859887 | + | Gm28802 | NNNGAGTGGAAAGCGACTGG | NAG | 2 | 0.1296 | Tier II |
10 | NC_000069.6 | 3 | 105981570 | - | Ovgp1 | NNNGAGTGGAAAGCGACTGG | NAG | 2 | 0.1296 | Tier II |
11 | NC_000070.6 | 4 | 102774910 | + | Sgip1 | NNNCAGTGGAAAGCCACTGG | NAG | 2 | 0.0707 | Tier II |
12 | NC_000075.6 | 9 | 119536354 | + | Scn5a | NNNCAGTGGAAAGCTACTGG | NGA | 2 | 0.0099 | Tier II |
13 | NC_000079.6 | 13 | 24504555 | - | Ripor2 | NNNCAGTGGAAAGCGCCTTG | NGG | 2 | 0.0 | Tier II |
14 | NC_000067.6 | 1 | 46544593 | - | Dnah7c | NNNCAGTGGAAAGCGTCTGG | NAG | 2 | 0.0 | Tier II |
15 | NC_000084.6 | 18 | 35847933 | - | Cxxc5 | NNNCAGTGGAGAGGGACTGG | NGG | 2 | 0.0 | Tier II |
16 | NC_000076.6 | 10 | 60113347 | + | Gm51801 | NNNCAGTGGAAAGCGACAGT | NGG | 2 | 0.4667 | Tier III |
17 | NC_000068.7 | 2 | 173648334 | - | Ppp4r1l-ps | NNNCAGTGGACAGAGACTGG | NGG | 2 | 0.14 | Tier III |