Construct: sgRNA BRDN0001149050
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGCTACGGATGATTTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA4 (8986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77921
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
64361518 |
- |
RPS6KA4 |
NNNGCTACGGATGATTTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
9767052 |
+ |
TNKS |
NNNGCTAAGGATGATTTCTG |
NGG |
2 |
0.4333 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6837788 |
+ |
Rps6ka4 |
NNNGCTTCGGATGATTTCGG |
NAG |
2 |
0.1134 |
Tier I |
| 2 |
NC_000072.6 |
6 |
112921420 |
+ |
Srgap3 |
NNNGCTATGGATGATTTCGC |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000081.6 |
15 |
98061688 |
- |
Senp1 |
NNNGCTACTGATGATTTCTG |
NGG |
2 |
0.3556 |
Tier II |
Other clones with same target sequence:
(none)
