Construct: sgRNA BRDN0001149054
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGCGATATTACTGCCACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3C2B (5287)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76712
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
204446095 |
- |
PIK3C2B |
NNNGCGATATTACTGCCACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
145870154 |
+ |
ZNF827 |
NNNGCGATATTGCTGCCATT |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000001.11 |
1 |
204683595 |
+ |
LRRN2 |
NNNGAGATCTTACTGCCACT |
NGG |
2 |
0.3265 |
Tier II |
| 4 |
NC_000002.12 |
2 |
3052373 |
+ |
LINC01250 |
NNNGCCATCTTACTGCCACT |
NGG |
2 |
0.3896 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
135710391 |
- |
Mki67 |
NNNGCAAGATTACTGCCACT |
NGG |
2 |
0.7333 |
Tier II |
| 2 |
NC_000074.6 |
8 |
91274081 |
+ |
Rpgrip1l |
NNNGTGAGATTACTGCCACT |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000072.6 |
6 |
36479171 |
- |
Chrm2 |
NNNGCGATGTTACTGCCACT |
NGA |
2 |
0.0463 |
Tier II |
| 4 |
NC_000081.6 |
15 |
89589977 |
- |
Rabl2 |
NNNGCGATATTACTGCCTCT |
NTG |
2 |
0.0195 |
Tier II |
| 5 |
NC_000074.6 |
8 |
91274081 |
+ |
Gm36163 |
NNNGTGAGATTACTGCCACT |
NGG |
2 |
0.4667 |
Tier III |
Other clones with same target sequence:
(none)
