Construct: sgRNA BRDN0001149056

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCCAATGGAACCCACCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALDH18A1 (5832)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77994

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 95627523 - ALDH18A1 NNNCAATGGAACCCACCCAA NGG 0 1.0 Tier I
2 NC_000019.10 19 57218816 - ZNF264 NNNCAAAAGAACCCACCCAA NGG 2 0.875 Tier II
3 NC_000005.10 5 167012677 + TENM2 NNNCAAAGGAACCCAACCAA NGG 2 0.875 Tier II
4 NC_000009.12 9 18073532 - ADAMTSL1 NNNCAATGGACCCCACCCAA NGG 1 0.4 Tier II
5 NC_000011.10 11 118350187 - CD3G NNNCCATGGAGCCCACCCAA NGG 2 0.325 Tier II
6 NC_000010.11 10 77376377 + KCNMA1 NNNCAATGGAACACACCAAA NGG 2 0.2071 Tier II
7 NC_000007.14 7 39465360 - POU6F2 NNNCAATGGTACCCACCCCA NGG 2 0.1826 Tier II
8 NC_000014.9 14 47569548 + MDGA2 NNNCAATGGAAACCAGCCAA NGG 2 0.1099 Tier II
9 NC_000002.12 2 198018450 + PLCL1 NNNCAATGGATCCCACCCCA NGG 2 0.0637 Tier II
10 NC_000007.14 7 121293449 - CPED1 NNNCAATGGATCCCACCCCA NGG 2 0.0637 Tier II
11 NC_000006.12 6 42400091 + TRERF1 NNNCAATGGAACCCACCCCA NAG 2 0.0536 Tier II
12 NC_000005.10 5 41390282 - PLCXD3 NNNCAATGGAAACCACCCAA NTG 2 0.0278 Tier II
13 NC_000013.11 13 87679179 + SLITRK5 NNNCAATGCAACCCACCCAA NTG 2 0.021 Tier II
14 NC_000019.10 19 14627676 - ADGRE3 NNNCAATGGAACCCAGCCAA NCG 2 0.0165 Tier II
15 NC_000007.14 7 40819169 - SUGCT NNNCAATGGAACCAACCCAA NTG 2 0.0136 Tier II
16 NC_000012.12 12 127943799 + LINC00507 NNNCAATGGTAACCACCCAA NGG 2 0.6303 Tier III
17 NC_000012.12 12 127943799 + LINC00508 NNNCAATGGTAACCACCCAA NGG 2 0.6303 Tier III
18 NC_000010.11 10 121026382 - LOC105378521 NNNCTATGGAACCTACCCAA NGG 2 0.2667 Tier III
19 NC_000010.11 10 77376377 + KCNMA1-AS3 NNNCAATGGAACACACCAAA NGG 2 0.2071 Tier III
20 NC_000007.14 7 39465360 - LOC105375238 NNNCAATGGTACCCACCCCA NGG 2 0.1826 Tier III
21 NC_000013.11 13 91130276 + LINC00379 NNNCAATGGAACCCAGCCGA NGG 2 0.0577 Tier III
22 NC_000013.11 13 91130276 + LOC105370312 NNNCAATGGAACCCAGCCGA NGG 2 0.0577 Tier III
23 NC_000005.10 5 41390282 - LOC105374740 NNNCAATGGAAACCACCCAA NTG 2 0.0278 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 40569114 - Aldh18a1 NNNCAACGGGACCCACCCAA NGG 2 0.3819 Tier I
2 NC_000072.6 6 48457332 + Sspo NNNCAATGGTACCCACCCAA NGC 2 0.0196 Tier I
3 NC_000073.6 7 141701966 - Muc2 NNNCAAAGGAACCCACCCAT NGG 2 0.525 Tier II
4 NC_000077.6 11 113815705 - Sdk2 NNNTAATGGGACCCACCCAA NGG 2 0.4444 Tier II
5 NC_000075.6 9 96167265 - Gk5 NNNCAATGGAAACAACCCAA NGG 2 0.25 Tier II
6 NC_000077.6 11 102275363 - Asb16 NNNCAATGAAACCAACCCAA NGG 2 0.225 Tier II
7 NC_000069.6 3 94478058 + Riiad1 NNNCAATGGACCCCACCCTA NGG 2 0.2154 Tier II
8 NC_000084.6 18 13810677 - Zfp521 NNNCAATTGAACCCACGCAA NGG 2 0.0368 Tier II
9 NC_000080.6 14 103292398 + Mycbp2 NNNCAATGGAACCCACCCAA NTA 2 0.0 Tier II
10 NC_000079.6 13 84866329 - Gm34196 NNNCAATGGAATCCAACCAA NGG 2 0.5385 Tier III
11 NC_000081.6 15 88301456 + 4930445N06Rik NNNCACAGGAACCCACCCAA NGG 2 0.3977 Tier III
12 NC_000068.7 2 75168411 + 2600014E21Rik NNNCCATGGAACCCACCCAG NGG 2 0.3824 Tier III
13 NC_000078.6 12 8765935 - Gm4755 NNNCCCTGGAACCCACCCAA NGG 2 0.2273 Tier III
14 NC_000079.6 13 110010618 - Gm32881 NNNTAATGGAACCCACCCAC NGG 2 0.1818 Tier III
15 NC_000083.6 17 13191924 + Gm36684 NNNCAATGAAACCCACCCCA NGG 2 0.133 Tier III
16 NC_000070.6 4 63750856 + Gm42294 NNNCAATGGAAGCCACCGAA NGG 2 0.0593 Tier III
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Other clones with same target sequence:

(none)