Construct: sgRNA BRDN0001149058

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAGTAGCTCCAATTCCGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYT1 (4661)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76130

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 64213634 + MYT1 NNNGTAGCTCCAATTCCGAT NGG 0 1.0 Tier I
2 NC_000004.12 4 164110112 - MARCHF1 NNNGTATCTCCAATTCCAAT NGG 2 0.3956 Tier II
3 NC_000003.12 3 181098304 - SOX2-OT NNNGTAGCTCCCATTCCTAT NGG 2 0.1754 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 116200158 - Ccdc60 NNNGTAACTCCAATTCCAAT NGG 2 0.6923 Tier II
2 NC_000082.6 16 24553709 + Lpp NNNGTAGCTCCAATTCCAAA NGG 2 0.3894 Tier II
3 NC_000069.6 3 86075772 + Sh3d19 NNNGTAACTCCAATTCCGAT NGA 2 0.0694 Tier II
4 NC_000075.6 9 13694731 + Maml2 NNNGTAACTCCAAGTCCGAT NGG 2 0.0 Tier II
5 NC_000071.6 5 116200158 - Gm36552 NNNGTAACTCCAATTCCAAT NGG 2 0.6923 Tier III
6 NC_000068.7 2 48433827 + Gm13481 NNNGTAGCTCCTATTCCGAT NAG 2 0.0864 Tier III
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Other clones with same target sequence:

(none)