Construct: sgRNA BRDN0001149058
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGTAGCTCCAATTCCGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MYT1 (4661)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76130
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
64213634 |
+ |
MYT1 |
NNNGTAGCTCCAATTCCGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
164110112 |
- |
MARCHF1 |
NNNGTATCTCCAATTCCAAT |
NGG |
2 |
0.3956 |
Tier II |
| 3 |
NC_000003.12 |
3 |
181098304 |
- |
SOX2-OT |
NNNGTAGCTCCCATTCCTAT |
NGG |
2 |
0.1754 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
116200158 |
- |
Ccdc60 |
NNNGTAACTCCAATTCCAAT |
NGG |
2 |
0.6923 |
Tier II |
| 2 |
NC_000082.6 |
16 |
24553709 |
+ |
Lpp |
NNNGTAGCTCCAATTCCAAA |
NGG |
2 |
0.3894 |
Tier II |
| 3 |
NC_000069.6 |
3 |
86075772 |
+ |
Sh3d19 |
NNNGTAACTCCAATTCCGAT |
NGA |
2 |
0.0694 |
Tier II |
| 4 |
NC_000075.6 |
9 |
13694731 |
+ |
Maml2 |
NNNGTAACTCCAAGTCCGAT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000071.6 |
5 |
116200158 |
- |
Gm36552 |
NNNGTAACTCCAATTCCAAT |
NGG |
2 |
0.6923 |
Tier III |
| 6 |
NC_000068.7 |
2 |
48433827 |
+ |
Gm13481 |
NNNGTAGCTCCTATTCCGAT |
NAG |
2 |
0.0864 |
Tier III |
Other clones with same target sequence:
(none)
