Construct: sgRNA BRDN0001149060
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTAAATGCTAAGAAGCGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK2 (8445)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77538
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
67657480 |
+ |
DYRK2 |
NNNAATGCTAAGAAGCGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
54207533 |
+ |
RPS9 |
NNNAATGCCAAGAAGGGCCA |
NGG |
2 |
0.142 |
Tier I |
| 3 |
NC_000013.11 |
13 |
74013328 |
+ |
KLF12 |
NNNAATGCTAAAAAGAGCCA |
NGG |
2 |
0.9333 |
Tier II |
| 4 |
NC_000010.11 |
10 |
71401300 |
- |
CDH23 |
NNNAATGGTAAGAAGCACCA |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000007.14 |
7 |
43948936 |
- |
UBE2D4 |
NNNACTGCTGAGAAGCGCCA |
NGG |
2 |
0.2778 |
Tier II |
| 6 |
NC_000005.10 |
5 |
133580548 |
+ |
FSTL4 |
NNNCATGCTAAGAAGTGCCA |
NGG |
2 |
0.1086 |
Tier II |
| 7 |
NC_000011.10 |
11 |
14877859 |
- |
PDE3B |
NNNAATGCTAAGAAGGGTCA |
NGG |
2 |
0.0989 |
Tier II |
| 8 |
NC_000011.10 |
11 |
14877859 |
- |
CYP2R1 |
NNNAATGCTAAGAAGGGTCA |
NGG |
2 |
0.0989 |
Tier II |
| 9 |
NC_000016.10 |
16 |
56904610 |
- |
SLC12A3 |
NNNCATGCTAAGAATCGCCA |
NGG |
2 |
0.0504 |
Tier II |
| 10 |
NC_000007.14 |
7 |
5693510 |
+ |
RNF216 |
NNNAATGCTAAGAAGCTCCA |
NGA |
2 |
0.0174 |
Tier II |
| 11 |
NC_000007.14 |
7 |
43948936 |
- |
POLR2J4 |
NNNACTGCTGAGAAGCGCCA |
NGG |
2 |
0.2778 |
Tier III |
| 12 |
NC_000011.10 |
11 |
61589809 |
- |
LOC101927495 |
NNNAATGCTGAGAAGTGCCA |
NGG |
2 |
0.1709 |
Tier III |
| 13 |
NC_000022.11 |
22 |
41064594 |
+ |
RPS9P2 |
NNNAATGCCAAGAAGGGCCA |
NGG |
2 |
0.142 |
Tier III |
| 14 |
NC_000005.10 |
5 |
133580548 |
+ |
LOC124901066 |
NNNCATGCTAAGAAGTGCCA |
NGG |
2 |
0.1086 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
118860786 |
- |
Dyrk2 |
NNNAATGCAAAGAAACGCCA |
NGG |
2 |
0.8739 |
Tier I |
| 2 |
NC_000068.7 |
2 |
5039490 |
- |
Optn |
NNNAAAGCTAAGAAGCGTCA |
NGG |
2 |
0.5571 |
Tier II |
| 3 |
NC_000075.6 |
9 |
60845452 |
+ |
Uaca |
NNNAATTCTAAGAAGCACCA |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000076.6 |
10 |
99014326 |
- |
Atp2b1 |
NNNAATGCTAAGATGCGCCT |
NGG |
2 |
0.32 |
Tier II |
| 5 |
NC_000069.6 |
3 |
135293721 |
+ |
Bdh2 |
NNNAATGCTAAGAAATGCCA |
NGG |
2 |
0.2896 |
Tier II |
| 6 |
NC_000074.6 |
8 |
104191691 |
- |
Bean1 |
NNNAATGATAAGAAGCCCCA |
NGG |
2 |
0.1529 |
Tier II |
| 7 |
NC_000078.6 |
12 |
36015432 |
+ |
Tspan13 |
NNNAATGCTAAGAAAGGCCA |
NGG |
2 |
0.1448 |
Tier II |
| 8 |
NC_000081.6 |
15 |
89585221 |
+ |
Rabl2 |
NNNAATGCTATGAAGTGCCA |
NGG |
2 |
0.0947 |
Tier II |
| 9 |
NC_000068.7 |
2 |
21674424 |
+ |
Gpr158 |
NNNATTGCTAAGAAGCTCCA |
NGG |
2 |
0.0909 |
Tier II |
| 10 |
NC_000068.7 |
2 |
14719496 |
- |
Cacnb2 |
NNNAATGCTCAGAAGCGCCA |
NGC |
2 |
0.0074 |
Tier II |
| 11 |
NC_000077.6 |
11 |
43137938 |
- |
Atp10b |
NNNAATGCTAAGAAGTGCCA |
NGT |
2 |
0.005 |
Tier II |
| 12 |
NC_000075.6 |
9 |
62414493 |
+ |
Gm40523 |
NNNACTGCTAGGAAGCGCCA |
NGG |
2 |
0.325 |
Tier III |
| 13 |
NC_000069.6 |
3 |
135293721 |
+ |
Gm46826 |
NNNAATGCTAAGAAATGCCA |
NGG |
2 |
0.2896 |
Tier III |
| 14 |
NC_000085.6 |
19 |
61256298 |
+ |
Gm18999 |
NNNAATGCTAAGCAGAGCCA |
NGG |
2 |
0.2105 |
Tier III |
| 15 |
NC_000081.6 |
15 |
39053329 |
- |
Gm7194 |
NNNAATGCTAAGAAGCGCCA |
NAA |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
