Construct: sgRNA BRDN0001149070

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGGAGGTATTAGACCATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C2A (5286)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76417

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 17168877 - PIK3C2A NNNGGAGGTATTAGACCATG NGG 0 1.0 Tier I
2 NC_000008.11 8 123121480 + TBC1D31 NNNGGAGGTATTGGACCATG NGG 1 0.6522 Tier II
3 NC_000012.12 12 45281649 - ANO6 NNNGGAGGTAATGGACCATG NGG 2 0.4891 Tier II
4 NC_000001.11 1 47972462 + TRABD2B NNNGGAGGTAATAGGCCATG NGG 2 0.4875 Tier II
5 NC_000012.12 12 12690537 - GPR19 NNNGGAGGCAGTAGACCATG NGG 2 0.3692 Tier II
6 NC_000011.10 11 94436271 + MRE11 NNNTGAGGTATCAGACCATG NGG 2 0.3445 Tier II
7 NC_000008.11 8 14497738 - SGCZ NNNGGAGGTATTAGATCATG NGG 1 0.3077 Tier II
8 NC_000010.11 10 116453571 + PNLIPRP3 NNNGGAGGTATTAGATCATG NGG 1 0.3077 Tier II
9 NC_000012.12 12 129162584 + TMEM132D NNNGGAGGTATTAGATCATG NGG 1 0.3077 Tier II
10 NC_000015.10 15 92093444 - SLCO3A1 NNNGGAGGTATTAGATCATG NGG 1 0.3077 Tier II
11 NC_000017.11 17 39325963 + FBXL20 NNNAGAGGTATTAGATCATG NGG 2 0.2769 Tier II
12 NC_000010.11 10 73585579 - USP54 NNNGAAGGTATTAGATCATG NGG 2 0.2667 Tier II
13 NC_000003.12 3 32896735 - TRIM71 NNNGGAGGTCTAAGACCATG NGG 2 0.2667 Tier II
14 NC_000009.12 9 137983822 + CACNA1B NNNGGAGGTATTAGACCAGG NGG 1 0.25 Tier II
15 NC_000003.12 3 121385460 - STXBP5L NNNGGACGTATTAGATCATG NGG 2 0.2115 Tier II
16 NC_000001.11 1 74156095 + LRRIQ3 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
17 NC_000001.11 1 147292840 - CHD1L NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
18 NC_000003.12 3 185753818 + IGF2BP2 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
19 NC_000007.14 7 131118057 + MKLN1 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
20 NC_000008.11 8 120699350 + SNTB1 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
21 NC_000010.11 10 127914028 + PTPRE NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
22 NC_000015.10 15 20840168 - POTEB2 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
23 NC_000015.10 15 21413806 - POTEB3 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
24 NC_000015.10 15 21851121 - POTEB NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
25 NC_000018.10 18 14516574 - POTEC NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
26 NC_000021.9 21 13636762 + POTED NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
27 NT_167208.1 112766 - LOC100288966 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
28 NT_187382.1 15 141000 - LOC102723502 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier II
29 NC_000023.11 X 15485408 - PIR NNNGGAGGTATTAGGTCATG NGG 2 0.2 Tier II
30 NC_000007.14 7 36665023 - AOAH NNNGGAGGTATTAGATAATG NGG 2 0.1436 Tier II
31 NC_000008.11 8 113052844 + CSMD3 NNNAGAGGTATTAGAGCATG NGG 2 0.1385 Tier II
32 NC_000012.12 12 120073778 + BICDL1 NNNGGAGGTATTATACTATG NGG 2 0.1244 Tier II
33 NC_000014.9 14 104707113 + INF2 NNNTGAGGTCTTAGACCATG NGG 2 0.1212 Tier II
34 NC_000006.12 6 11377868 + NEDD9 NNNGGAGGTATTGGAGCATG NGG 2 0.1003 Tier II
35 NC_000004.12 4 142274396 - INPP4B NNNGGAGTTATTAGAGCATG NGG 2 0.0962 Tier II
36 NC_000002.12 2 144276781 - GTDC1 NNNGGAGGTATTAGGCCATG NGA 2 0.0451 Tier II
37 NC_000002.12 2 226905470 + RHBDD1 NNNGGAGGTAGTAGACGATG NGG 2 0.0235 Tier II
38 NC_000006.12 6 153391793 + LOC105378066 NNNAGAGGTATTAGATCATG NGG 2 0.2769 Tier III
39 NC_000002.12 2 230090511 + LOC107985995 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
40 NC_000003.12 3 185753818 + LOC124906313 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
41 NC_000006.12 6 112738525 - LOC105377949 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
42 NC_000006.12 6 112738525 - LOC107986634 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
43 NC_000012.12 12 94519251 - LOC102724960 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
44 NC_000013.11 13 63259563 - LINC00376 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
45 NC_000018.10 18 51426666 + LINC01630 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
46 NC_000018.10 18 52057440 + LOC105372121 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
47 NC_000021.9 21 32926674 - LOC105377136 NNNGGAGGTATTGGATCATG NGG 2 0.2007 Tier III
48 NC_000018.10 18 14742478 - LOC105372007 NNNGGAGGTATTAGGTCATG NGG 2 0.2 Tier III
49 NC_000023.11 X 15485408 - PIR-FIGF NNNGGAGGTATTAGGTCATG NGG 2 0.2 Tier III
50 NT_187383.1 16 1803170 - LOC107987386 NNNGGAGGTATTAGGTCATG NGG 2 0.2 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 68982532 - Adcy2 NNNGGAGGTAGAAGACCATG NGG 2 0.32 Tier I
2 NC_000076.6 10 116330168 + Ptprb NNNGGAAGTATTAGACCATA NGG 2 0.9375 Tier II
3 NC_000070.6 4 43095053 - Unc13b NNNGGAGGTATTGGAACATG NGG 2 0.6522 Tier II
4 NC_000075.6 9 79765947 - Cox7a2 NNNGGAGGGATCAGACCATG NGG 2 0.5865 Tier II
5 NC_000073.6 7 139872042 - Adgra1 NNNGGAGGTATTACAACATG NGG 2 0.4286 Tier II
6 NC_000078.6 12 91671525 - Ston2 NNNGGAGGTATTACAACATG NGG 2 0.4286 Tier II
7 NC_000077.6 11 51374157 - Col23a1 NNNGGAGGTGTTAGGCCATG NGG 2 0.3611 Tier II
8 NC_000084.6 18 74008857 + Mapk4 NNNGGAGGTATAAGACCGTG NGG 2 0.32 Tier II
9 NC_000069.6 3 103875694 + Ptpn22 NNNGGAGGGATTAGACTATG NGG 2 0.2889 Tier II
10 NC_000071.6 5 96801678 + Anxa3 NNNAGAGGTATTAGATCATG NGG 2 0.2769 Tier II
11 NC_000083.6 17 11877759 + Prkn NNNGGAAGTATTAGACCATG NAG 2 0.2593 Tier II
12 NC_000079.6 13 106926572 + Ipo11 NNNGTAGGTATTAGACCATC NGG 2 0.1286 Tier II
13 NC_000084.6 18 3298512 + Crem NNNGGAGGTATTAGAACATG NGC 2 0.0222 Tier II
14 NC_000082.6 16 5343906 - Gm41414 NNNGGAGGGATGAGACCATG NGG 2 0.3095 Tier III
15 NC_000083.6 17 11877759 + D17Ertd648e NNNGGAAGTATTAGACCATG NAG 2 0.2593 Tier III
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Other clones with same target sequence:

(none)