Construct: sgRNA BRDN0001149078

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAAAACTCCTATTATCCACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AAK1 (22848)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77937

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 69542537 - AAK1 NNNAACTCCTATTATCCACC NGG 0 1.0 Tier I
2 NC_000008.11 8 97962193 - MATN2 NNNAACACCTACTATCCACC NGG 2 0.8289 Tier II
3 NC_000011.10 11 114610576 - NXPE4 NNNAATACCTATTATCCACC NGG 2 0.8125 Tier II
4 NC_000011.10 11 114610576 - NXPE2 NNNAATACCTATTATCCACC NGG 2 0.8125 Tier II
5 NC_000011.10 11 114604065 - NXPE4 NNNAATTCTTATTATCCACC NGG 2 0.8125 Tier II
6 NC_000011.10 11 114604065 - NXPE2 NNNAATTCTTATTATCCACC NGG 2 0.8125 Tier II
7 NC_000011.10 11 114604485 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
8 NC_000011.10 11 114604485 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
9 NC_000011.10 11 114605489 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
10 NC_000011.10 11 114605489 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
11 NC_000011.10 11 114605613 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
12 NC_000011.10 11 114605613 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
13 NC_000011.10 11 114605864 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
14 NC_000011.10 11 114605864 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
15 NC_000011.10 11 114605948 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
16 NC_000011.10 11 114605948 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
17 NC_000011.10 11 114606616 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
18 NC_000011.10 11 114606616 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
19 NC_000011.10 11 114607747 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
20 NC_000011.10 11 114607747 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
21 NC_000011.10 11 114608834 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
22 NC_000011.10 11 114608834 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
23 NC_000011.10 11 114608999 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
24 NC_000011.10 11 114608999 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
25 NC_000011.10 11 114610282 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
26 NC_000011.10 11 114610282 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
27 NC_000011.10 11 114610450 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
28 NC_000011.10 11 114610450 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
29 NC_000011.10 11 114610534 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
30 NC_000011.10 11 114610534 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
31 NC_000011.10 11 114610618 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
32 NC_000011.10 11 114610618 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
33 NC_000011.10 11 114611080 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
34 NC_000011.10 11 114611080 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
35 NC_000011.10 11 114611364 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
36 NC_000011.10 11 114611364 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
37 NC_000011.10 11 114611784 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
38 NC_000011.10 11 114611784 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
39 NC_000011.10 11 114612414 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
40 NC_000011.10 11 114612414 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
41 NC_000011.10 11 114612787 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
42 NC_000011.10 11 114612787 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
43 NC_000011.10 11 114613421 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
44 NC_000011.10 11 114613421 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
45 NC_000011.10 11 114613755 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
46 NC_000011.10 11 114613755 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
47 NC_000011.10 11 114614709 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
48 NC_000011.10 11 114614709 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
49 NC_000011.10 11 114615542 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
50 NC_000011.10 11 114615542 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
51 NC_000011.10 11 114615626 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
52 NC_000011.10 11 114615626 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
53 NC_000011.10 11 114616925 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
54 NC_000011.10 11 114616925 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
55 NC_000011.10 11 114619943 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
56 NC_000011.10 11 114619943 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
57 NC_000011.10 11 114620488 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
58 NC_000011.10 11 114620488 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
59 NC_000011.10 11 114620785 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
60 NC_000011.10 11 114620785 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
61 NC_000011.10 11 114621204 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
62 NC_000011.10 11 114621204 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
63 NC_000011.10 11 114622207 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
64 NC_000011.10 11 114622207 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
65 NC_000011.10 11 114623000 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
66 NC_000011.10 11 114623000 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
67 NC_000011.10 11 114623042 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
68 NC_000011.10 11 114623042 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
69 NC_000011.10 11 114623126 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
70 NC_000011.10 11 114623126 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
71 NC_000011.10 11 114623838 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
72 NC_000011.10 11 114623838 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
73 NC_000011.10 11 114624006 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
74 NC_000011.10 11 114624006 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
75 NC_000011.10 11 114624300 - NXPE4 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
76 NC_000011.10 11 114624300 - NXPE2 NNNAACACTTATTATCCACC NGG 2 0.7656 Tier II
77 NC_000001.11 1 16975169 - MFAP2 NNNAACACATATTATCCACC NGG 2 0.75 Tier II
78 NC_000011.10 11 114607246 - NXPE4 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
79 NC_000011.10 11 114607246 - NXPE2 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
80 NC_000011.10 11 114608205 - NXPE4 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
81 NC_000011.10 11 114608205 - NXPE2 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
82 NC_000011.10 11 114611658 - NXPE4 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
83 NC_000011.10 11 114611658 - NXPE2 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
84 NC_000011.10 11 114621246 - NXPE4 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
85 NC_000011.10 11 114621246 - NXPE2 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
86 NC_000011.10 11 114622709 - NXPE4 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
87 NC_000011.10 11 114622709 - NXPE2 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
88 NC_000011.10 11 114624928 - NXPE4 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
89 NC_000011.10 11 114624928 - NXPE2 NNNAACACGTATTATCCACC NGG 2 0.5417 Tier II
90 NC_000017.11 17 1288143 + TRARG1 NNNAACCCCTTTTATCCACC NGG 2 0.2115 Tier II
91 NC_000009.12 9 83395012 + FRMD3 NNNAACACCTATTATGCACC NGG 2 0.1346 Tier II
92 NC_000012.12 12 11825419 - ETV6 NNNGACTCCTATTATCCACG NGG 2 0.0368 Tier II
93 NC_000002.12 2 84817300 + DNAH6 NNNAACTCCCATTATCCACC NTG 2 0.0208 Tier II
94 NC_000012.12 12 117522658 - KSR2 NNNAACTCCTATTATCCACT NGA 2 0.0208 Tier II
95 NC_000018.10 18 47823443 + SMAD2 NNNAACTCCTGTTATCCACC NGC 2 0.0144 Tier II
96 NC_000015.10 15 56587273 + LOC105370832 NNNCACTTCTATTATCCACC NGG 2 0.3088 Tier III
97 NC_000004.12 4 184275555 - LOC105377582 NNNAACTCCTATTTTCCTCC NGG 2 0.2667 Tier III
98 NC_000023.11 X 132983788 - LOC124905219 NNNAATTCCTATTATGCACC NGG 2 0.1429 Tier III
99 NC_000002.12 2 16385823 + LOC107985855 NNNAACTCCTATTATGCTCC NGG 2 0.0769 Tier III
100 NC_000018.10 18 44492918 - LINC01478 NNNAACCCCTATTATCCACC NGT 2 0.0111 Tier III
101 NC_000010.11 10 108288814 + LOC105378476 NNNAACTCCTATTATCCACC NTT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 86937071 + Aak1 NNNAACTCCCATTATCCACC NGG 1 0.5333 Tier I
2 NC_000076.6 10 39370389 - Fyn NNNAATTCCTATTTTCCACC NGG 2 0.4952 Tier II
3 NC_000075.6 9 14323357 + Sesn3 NNNAACTCTTATTATCAACC NGG 2 0.4083 Tier II
4 NC_000073.6 7 105570923 + Apbb1 NNNAACTCCTAGAATCCACC NGG 2 0.3462 Tier II
5 NC_000084.6 18 64262244 - St8sia3 NNNAACTCCTAGTATCCACA NGG 2 0.25 Tier II
6 NC_000068.7 2 158943628 - Gm38630 NNNCACTCCTACTATCCACC NGG 2 0.3344 Tier III
7 NC_000084.6 18 64262244 - St8sia3os NNNAACTCCTAGTATCCACA NGG 2 0.25 Tier III
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Other clones with same target sequence:

(none)