Construct: sgRNA BRDN0001149079
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATTTGAGATGGCATTCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA5 (2044)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76678
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
65490693 |
+ |
EPHA5 |
NNNTTTGAGATGGCATTCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
23643940 |
+ |
KLHL29 |
NNNCTTGAGATGGCATTTCG |
NGG |
2 |
0.416 |
Tier II |
| 3 |
NC_000015.10 |
15 |
31433678 |
- |
KLF13 |
NNNTTTGAGATGACATTCCC |
NGG |
2 |
0.3956 |
Tier II |
| 4 |
NC_000015.10 |
15 |
25430527 |
- |
UBE3A |
NNNTTTGACATGGCATTCCT |
NGG |
2 |
0.3769 |
Tier II |
| 5 |
NC_000003.12 |
3 |
61862410 |
+ |
PTPRG |
NNNTTTGAGATGGAATTCCG |
NTG |
2 |
0.0136 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
84233899 |
+ |
Epha5 |
NNNTTTGAGATGGCATTCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
103205896 |
+ |
Prima1 |
NNNTTTGAGATGGCAATCCT |
NGG |
2 |
0.6364 |
Tier II |
| 3 |
NC_000079.6 |
13 |
42985237 |
- |
Phactr1 |
NNNGTTGAGATGGCATTCCA |
NGG |
2 |
0.5859 |
Tier II |
| 4 |
NC_000072.6 |
6 |
65920549 |
+ |
Prdm5 |
NNNTTTGAGGTGGCATTCCA |
NGG |
2 |
0.5208 |
Tier II |
| 5 |
NC_000073.6 |
7 |
135525735 |
+ |
Clrn3 |
NNNCTGGAGATGGCATTCCG |
NGG |
2 |
0.3697 |
Tier II |
| 6 |
NC_000071.6 |
5 |
131541873 |
+ |
Auts2 |
NNNTTTGAGATGGTATTCAG |
NGG |
2 |
0.3143 |
Tier II |
| 7 |
NC_000067.6 |
1 |
156321172 |
+ |
Nphs2 |
NNNTTTGAGATGCCATTCCT |
NGG |
2 |
0.2947 |
Tier II |
| 8 |
NC_000072.6 |
6 |
148946139 |
- |
Sinhcaf |
NNNTTGGAGATGGCATTGCG |
NGG |
2 |
0.0762 |
Tier II |
| 9 |
NC_000085.6 |
19 |
44131722 |
- |
Cwf19l1 |
NNNTTTGAGATGGCATTCCT |
NTG |
2 |
0.0273 |
Tier II |
| 10 |
NC_000072.6 |
6 |
148946139 |
- |
3010003L21Rik |
NNNTTGGAGATGGCATTGCG |
NGG |
2 |
0.0762 |
Tier III |
Other clones with same target sequence:
(none)
