Construct: sgRNA BRDN0001149086
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAGAGTGGTTCGCACACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKM (5213)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77076
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
48140735 |
+ |
PFKM |
NNNGAGTGGTTCGCACACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
70408720 |
+ |
EIF4EBP2 |
NNNGAGTGGTTCGCACAAAA |
NGG |
2 |
0.5048 |
Tier II |
| 3 |
NC_000012.12 |
12 |
120151016 |
+ |
GCN1 |
NNNGACTGGGTCGCACACAG |
NGG |
2 |
0.3409 |
Tier II |
| 4 |
NC_000003.12 |
3 |
109148079 |
+ |
C3orf85 |
NNNGAGTGGTTAGCATACAG |
NGG |
2 |
0.2198 |
Tier II |
| 5 |
NC_000022.11 |
22 |
43413632 |
- |
MPPED1 |
NNNGCGTGGTTCCCACACAG |
NGG |
2 |
0.2105 |
Tier II |
| 6 |
NC_000002.12 |
2 |
232319258 |
+ |
DIS3L2 |
NNNGTGTGGTTGGCACACAG |
NGG |
2 |
0.1616 |
Tier II |
| 7 |
NC_000002.12 |
2 |
11783046 |
+ |
LPIN1 |
NNNTAGTGGTTGGCACACAG |
NGG |
2 |
0.1616 |
Tier II |
| 8 |
NC_000005.10 |
5 |
149038898 |
- |
SH3TC2 |
NNNGAGTGGTTCTCACACAC |
NGG |
2 |
0.1286 |
Tier II |
| 9 |
NC_000017.11 |
17 |
45139644 |
- |
ACBD4 |
NNNGAGTGTTTCGCACCCAG |
NGG |
2 |
0.0941 |
Tier II |
| 10 |
NC_000019.10 |
19 |
18103068 |
- |
MAST3 |
NNNGAGTGGTTCCCACCCAG |
NGG |
2 |
0.0743 |
Tier II |
| 11 |
NC_000019.10 |
19 |
44710573 |
- |
CEACAM16 |
NNNGAGTGGTTCCCACTCAG |
NGG |
2 |
0.0561 |
Tier II |
| 12 |
NC_000001.11 |
1 |
59701463 |
- |
FGGY |
NNNGAGTGGTTGGCACACAG |
NTG |
2 |
0.0173 |
Tier II |
| 13 |
NC_000003.12 |
3 |
173843177 |
+ |
NLGN1 |
NNNGAGTGGTTGGCACACAG |
NGC |
2 |
0.0099 |
Tier II |
| 14 |
NC_000004.12 |
4 |
49016322 |
+ |
CWH43 |
NNNGAGTGGTTCGCATACAG |
NGC |
2 |
0.0068 |
Tier II |
| 15 |
NC_000013.11 |
13 |
37998486 |
+ |
LINC02334 |
NNNGAGTGGTTTGCAAACAG |
NGG |
2 |
0.5385 |
Tier III |
| 16 |
NC_000005.10 |
5 |
72687281 |
- |
TNPO1-DT |
NNNGAGTGGTTCTCAAACAG |
NGG |
2 |
0.3 |
Tier III |
| 17 |
NC_000023.11 |
X |
96442853 |
- |
LOC107985714 |
NNNGAGTGGTTGGCACACAG |
NAG |
2 |
0.1152 |
Tier III |
| 18 |
NC_000002.12 |
2 |
23180805 |
- |
LOC107985792 |
NNNTAGTGGTTCTCACACAG |
NGG |
2 |
0.1091 |
Tier III |
| 19 |
NC_000019.10 |
19 |
44710573 |
- |
CEACAM16-AS1 |
NNNGAGTGGTTCCCACTCAG |
NGG |
2 |
0.0561 |
Tier III |
| 20 |
NC_000006.12 |
6 |
1038393 |
- |
LINC01622 |
NNNGAGTGGTTTGCACACAG |
NGA |
2 |
0.0374 |
Tier III |
| 21 |
NC_000004.12 |
4 |
49016322 |
+ |
TPI1P4 |
NNNGAGTGGTTCGCATACAG |
NGC |
2 |
0.0068 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
131506764 |
+ |
Srgap2 |
NNNGAGTGGTGAGCACACAG |
NGG |
2 |
0.2857 |
Tier II |
| 2 |
NC_000075.6 |
9 |
90195473 |
- |
Adamts7 |
NNNGGGTGGTTCGAACACAG |
NGG |
2 |
0.252 |
Tier II |
| 3 |
NC_000067.6 |
1 |
140612794 |
+ |
Kcnt2 |
NNNCAGTGGTTGGCACACAG |
NGG |
2 |
0.2353 |
Tier II |
| 4 |
NC_000071.6 |
5 |
27745155 |
+ |
Paxip1 |
NNNGAGTGGTTAGCCCACAG |
NGG |
2 |
0.1948 |
Tier II |
| 5 |
NC_000070.6 |
4 |
25252693 |
+ |
Ufl1 |
NNNGAGTGGCTCTCACACAG |
NGG |
2 |
0.16 |
Tier II |
| 6 |
NC_000078.6 |
12 |
86100730 |
+ |
Ift43 |
NNNGAGTGGGTCTCACACAG |
NGG |
2 |
0.15 |
Tier II |
| 7 |
NC_000071.6 |
5 |
27745072 |
+ |
Paxip1 |
NNNGAGTGGTTAGCTCACAG |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000068.7 |
2 |
58482878 |
- |
Acvr1 |
NNNGAGTGGTTCTCACTCAG |
NGG |
2 |
0.04 |
Tier II |
| 9 |
NC_000073.6 |
7 |
48976430 |
- |
Nav2 |
NNNGAGTGGTTCGCCCTCAG |
NGG |
2 |
0.0364 |
Tier II |
| 10 |
NC_000071.6 |
5 |
92868791 |
- |
Shroom3 |
NNNGAGTGGTTCGCAGCCAG |
NGG |
2 |
0.0271 |
Tier II |
| 11 |
NC_000082.6 |
16 |
37085729 |
+ |
Polq |
NNNGAGTGGTTCCCACACAG |
NTG |
2 |
0.0164 |
Tier II |
| 12 |
NC_000075.6 |
9 |
36689730 |
- |
1700027I24Rik |
NNNGAGTGTTTTGCACACAG |
NGG |
2 |
0.2872 |
Tier III |
| 13 |
NC_000067.6 |
1 |
140612794 |
+ |
4930590L20Rik |
NNNCAGTGGTTGGCACACAG |
NGG |
2 |
0.2353 |
Tier III |
| 14 |
NC_000070.6 |
4 |
25252693 |
+ |
LOC115489977 |
NNNGAGTGGCTCTCACACAG |
NGG |
2 |
0.16 |
Tier III |
| 15 |
NC_000075.6 |
9 |
46469082 |
- |
Gm31432 |
NNNGAGTGGTTCTCACACAG |
NAG |
2 |
0.0778 |
Tier III |
| 16 |
NC_000078.6 |
12 |
43828157 |
+ |
Gm33384 |
NNNGAGTGGTTCCCACACAG |
NCG |
2 |
0.0451 |
Tier III |
Other clones with same target sequence:
(none)
