Construct: sgRNA BRDN0001149089

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCACCATGAAAATATCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAA1 (5562)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76255

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 40775479 + PRKAA1 NNNACCATGAAAATATCAGA NGG 0 1.0 Tier I
2 NC_000006.12 6 39522196 + KIF6 NNNACAATGTAAATATCAGA NGG 2 0.8193 Tier II
3 NC_000006.12 6 70054786 + COL19A1 NNNACAATGTAAATATCAGA NGG 2 0.8193 Tier II
4 NC_000006.12 6 144341306 - UTRN NNNACAAAGAAAATATCAGA NGG 2 0.7429 Tier II
5 NC_000009.12 9 85622492 + AGTPBP1 NNNACCAGGAAAATATCAGA NGG 1 0.7333 Tier II
6 NC_000001.11 1 169822117 + FIRRM NNNACCAAGTAAATATCAGA NGG 2 0.7059 Tier II
7 NC_000003.12 3 32703754 - CNOT10 NNNAGTATGAAAATATCAGA NGG 2 0.5571 Tier II
8 NC_000007.14 7 108424712 + NRCAM NNNATCATGAAAATATCAGG NGG 2 0.4866 Tier II
9 NC_000003.12 3 107703933 - BBX NNNACCATGAAAATACCAAA NGG 2 0.4762 Tier II
10 NC_000023.11 X 11481154 - ARHGAP6 NNNACCATGAAAATATTAGA NGG 1 0.4667 Tier II
11 NC_000004.12 4 53210100 - SCFD2 NNNATCATGAAAATATCAAA NGG 2 0.4545 Tier II
12 NC_000006.12 6 45966888 - CLIC5 NNNACCATAAAAATATCAGT NGG 2 0.3857 Tier II
13 NC_000010.11 10 109876578 - XPNPEP1 NNNAGCATAAAAATATCAGA NGG 2 0.3857 Tier II
14 NC_000001.11 1 183987164 + COLGALT2 NNNTCCATGAAAATATCAGT NGG 2 0.3818 Tier II
15 NC_000004.12 4 158530429 - RXFP1 NNNACCATTAAAATATCAAA NGG 2 0.381 Tier II
16 NC_000006.12 6 143860289 - LTV1 NNNACCATTAAAATATCAGT NGG 2 0.32 Tier II
17 NC_000001.11 1 21720982 - USP48 NNNACCATGAAAATATAAGT NGG 2 0.28 Tier II
18 NC_000020.11 20 20306347 + CFAP61 NNNACCATGAAAATATCACT NGG 2 0.269 Tier II
19 NC_000002.12 2 133521109 + NCKAP5 NNNACAATGAAAATATCAGA NAG 2 0.2407 Tier II
20 NC_000022.11 22 45755242 - ATXN10 NNNACGATGAAAATATCGGA NGG 2 0.2 Tier II
21 NC_000010.11 10 82396149 - NRG3 NNNACCATGAAAATCTCAAA NGG 2 0.1948 Tier II
22 NC_000007.14 7 38573734 - AMPH NNNACCATGAAATAATCAGA NGG 2 0.1857 Tier II
23 NC_000012.12 12 100272439 - SCYL2 NNNACCATGAAAATTACAGA NGG 2 0.1818 Tier II
24 NC_000012.12 12 100272439 - DEPDC4 NNNACCATGAAAATTACAGA NGG 2 0.1818 Tier II
25 NC_000006.12 6 105322177 + PREP NNNACCATGAAAATACCAGA NAG 2 0.1728 Tier II
26 NC_000013.11 13 33834827 - RFC3 NNNACCATGAAAATACCAGA NAG 2 0.1728 Tier II
27 NC_000003.12 3 41849133 - ULK4 NNNACCATCAAAATCTCAGA NGG 2 0.1469 Tier II
28 NC_000011.10 11 18102876 - SAAL1 NNNACCATGATAATATTAGA NGG 2 0.1436 Tier II
29 NC_000005.10 5 5142110 + ADAMTS16 NNNACCATGAAAATTCCAGA NGG 2 0.1333 Tier II
30 NC_000013.11 13 72891930 + PIBF1 NNNACCTTGAAAACATCAGA NGG 2 0.125 Tier II
31 NC_000005.10 5 42707378 + GHR NNNACCATGAAAATATTAGA NAG 2 0.121 Tier II
32 NC_000010.11 10 96883544 - LCOR NNNACCATGCAACTATCAGA NGG 2 0.0702 Tier II
33 NC_000009.12 9 112383400 - HSDL2 NNNACCATAAAAATATCAGA NGA 2 0.0446 Tier II
34 NC_000007.14 7 81737553 + HGF NNNACCATGAAACTTTCAGA NGG 2 0.0421 Tier II
35 NC_000023.11 X 50611587 + SHROOM4 NNNACAATGAAAATATCAGA NGC 2 0.0206 Tier II
36 NC_000004.12 4 146506665 - SLC10A7 NNNACCATGAAAATATTAGA NTG 2 0.0182 Tier II
37 NC_000013.11 13 93857311 + GPC6 NNNACCATAAAAATATCAGA NGT 2 0.0104 Tier II
38 NC_000006.12 6 151305442 - AKAP12 NNNACCATGAAAAAATCAGA NGT 2 0.01 Tier II
39 NC_000001.11 1 3656928 - TP73 NNNACCATGAAAATATCAGT NGT 2 0.0097 Tier II
40 NC_000005.10 5 40775479 + LOC124900968 NNNACCATGAAAATATCAGA NGG 0 1.0 Tier III
41 NC_000021.9 21 28198007 + LINC01695 NNNACCATGAGAATAACAGA NGG 2 0.5909 Tier III
42 NC_000008.11 8 94025062 - LOC124901979 NNNAACAGGAAAATATCAGA NGG 2 0.419 Tier III
43 NC_000004.12 4 158530429 - LOC124900802 NNNACCATTAAAATATCAAA NGG 2 0.381 Tier III
44 NC_000008.11 8 33038038 + LOC105379362 NNNATCATGAAAATATCTGA NGG 2 0.3182 Tier III
45 NC_000008.11 8 117397290 + LOC105375716 NNNCCCATGAAGATATCAGA NGG 2 0.2549 Tier III
46 NC_000004.12 4 153968910 + LOC101927947 NNNACCCTGAAAATATCAGA NAG 2 0.1134 Tier III
47 NC_000005.10 5 164747065 + LINC03000 NNNACCATGATTATATCAGA NGG 2 0.1026 Tier III
48 NC_000003.12 3 191781817 - LOC105374276 NNNACCATGAAAATATCATA NGT 2 0.0108 Tier III
49 NC_000013.11 13 93857311 + GPC6-AS2 NNNACCATAAAAATATCAGA NGT 2 0.0104 Tier III
50 NC_000018.10 18 38483555 - LOC105372074 NNNACCATGATAATATCAGA NGT 2 0.005 Tier III
51 NC_000016.10 16 80344982 + DYNLRB2-AS1 NNNACCATGAAGAGATCAGA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 5164737 - Prkaa1 NNNACCATGAAAATATCAGA NGG 0 1.0 Tier I
2 NC_000069.6 3 32579279 - Gnb4 NNNACCATGAAAATATCTGA NGG 1 0.5 Tier II
3 NC_000083.6 17 42731123 - Adgrf2 NNNACCATAAAAATATCATA NGG 2 0.4286 Tier II
4 NC_000077.6 11 57986957 - Larp1 NNNACCATGAAAATACAAGA NGG 2 0.3111 Tier II
5 NC_000078.6 12 52970455 + Akap6 NNNTCCATGAAAATATAAGA NGG 2 0.297 Tier II
6 NC_000076.6 10 119789714 - Grip1 NNNACCTTGAGAATATCAGA NGG 2 0.2844 Tier II
7 NC_000085.6 19 45883687 + Armh3 NNNACCATGAAAATATTAGT NGG 2 0.28 Tier II
8 NC_000078.6 12 16934228 - Rock2 NNNACTATGAAAACATCAGA NGG 2 0.2653 Tier II
9 NC_000085.6 19 18825630 - Trpm6 NNNAACATGAAAATATCACA NGG 2 0.2562 Tier II
10 NC_000077.6 11 35154461 - Slit3 NNNACCAAGAAACTATCAGA NGG 2 0.1684 Tier II
11 NC_000071.6 5 19559221 - Magi2 NNNACCATTAAAATCTCAGA NGG 2 0.1455 Tier II
12 NC_000076.6 10 10802817 + Grm1 NNNACCATGAAAATATCCAA NGG 2 0.1361 Tier II
13 NC_000083.6 17 81701110 + Slc8a1 NNNACCATGAACATATCAGA NAG 2 0.0682 Tier II
14 NC_000085.6 19 42366011 - Crtac1 NNNACCATGAAACTCTCAGA NGG 2 0.0574 Tier II
15 NC_000080.6 14 66817503 + Dpysl2 NNNACCATAAAAATATCAGA NGA 2 0.0446 Tier II
16 NC_000076.6 10 18100288 + Reps1 NNNACCATGAAAAAATCAGA NGA 2 0.043 Tier II
17 NC_000068.7 2 90487815 + Ptprj NNNACCATGAAAGTATGAGA NGG 2 0.0384 Tier II
18 NC_000073.6 7 67573528 - Lrrc28 NNNACCAGGAAAATATCAGA NTG 2 0.0286 Tier II
19 NC_000084.6 18 78917480 + Setbp1 NNNACCATAAAAATATCAGA NTG 2 0.025 Tier II
20 NC_000068.7 2 31189408 - Gpr107 NNNACCATGAAAAAATCAGA NTG 2 0.0241 Tier II
21 NC_000075.6 9 13427385 - Maml2 NNNACAATGAAAATATCAGA NGT 2 0.015 Tier II
22 NC_000079.6 13 113999131 - Arl15 NNNACCATGAAATTATCAGA NGC 2 0.0067 Tier II
23 NC_000071.6 5 136853449 - Col26a1 NNNCCCATGAAAATATCAGA NGT 2 0.0057 Tier II
24 NC_000068.7 2 154241048 + Cdk5rap1 NNNACCAGGAAAAGATCAGA NGG 2 0.0 Tier II
25 NC_000076.6 10 127790547 - Rdh9 NNNACCATGAGAAGATCAGA NGG 2 0.0 Tier II
26 NC_000076.6 10 127813144 - Rdh16 NNNACCATGAGAAGATCAGA NGG 2 0.0 Tier II
27 NC_000086.7 X 155758471 - Gm15155 NNNACCATGAAAATAACAAA NGG 2 0.6494 Tier III
28 NC_000074.6 8 93619850 - Gm26843 NNNACCAGGAAAATATCAGG NGG 2 0.5608 Tier III
29 NC_000076.6 10 119789714 - Gm51815 NNNACCTTGAGAATATCAGA NGG 2 0.2844 Tier III
30 NC_000077.6 11 32311217 - Gm46275 NNNACCATGAAAACAGCAGA NGG 2 0.0989 Tier III
31 NC_000081.6 15 88426079 - Gm35831 NNNACCATGAAAATATCAGA NTG 1 0.039 Tier III
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Other clones with same target sequence:

(none)